Aldh1a3 (NM_053080) Mouse Tagged ORF Clone

CAT#: MG222097

Aldh1a3 (tGFP-tagged) - Mouse aldehyde dehydrogenase family 1 subfamily A3 (Aldh1a3), (10ug)

Product Images

Circular map for MG222097

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aldh1a3
• Synonyms: ALDH6; RALDH3; V1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222097 representing NM_053080
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACCACCAACGGGGCTGTGGAAAACGGACAGCCGGATGGGAAACCGCCTGCCTTGCCGCGCCCCA
  TCCGCAACTTGGAGGTCAAGTTCACCAAGATATTTATCAACAACGACTGGCACGAATCCAAGAGTGGAAG
  AAAGTTTGCCACATATAACCCTTCAACACTAGAGAAAATATGTGAGGTGGAAGAAGGAGATAAGCCCGAT
  GTGGACAAGGCTGTGGAGGCCGCTCAAGCTGCCTTCCAGCGGGGATCCCCGTGGCGCCGGCTGGATGCAC
  TGAGCAGAGGCCAGTTGCTGCATCAGCTGGCTGACCTTGTAGAAAGGGACCGAGCGATCCTGGCTACTCT
  GGAGACCATGGACACCGGCAAGCCATTCCTTCATGCCTTTTTCGTCGACCTGGAAGGCTGTATTAAGACC
  TTCAGATATTTTGCCGGGTGGGCAGACAAAATCCAGGGCAGGACCATCCCCACAGATGACAACGTTGTGT
  GCTTCACCAGGCATGAGCCCATCGGGGTGTGTGGGGCCATTACACCATGGAACTTCCCCCTGCTGATGCT
  GGCCTGGAAACTGGCTCCTGCCCTGTGCTGTGGGAACACCGTGGTCCTGAAGCCAGCTGAGCAGACCCCT
  CTCACGGCTCTGTACCTAGCCTCTCTCATCAAAGAGGTCGGGTTCCCTCCGGGTGTGGTGAACATTGTAC
  CAGGCTTTGGGCCCACTGTGGGAGCAGCAATTTCCTCCCATCCGCAGATCAACAAGATAGCCTTCACCGG
  CTCCACAGAGGTTGGAAAGCTGGTCAGAGAAGCCGCCTCCCGGAGCAACCTGAAGAGGGTCACACTGGAG
  CTAGGAGGCAAGAACCCGTGCATCGTGTGTGCAGATGCTGACTTGGACTTGGCCGTCGAGTGTGCTCACC
  AGGGAGTGTTCTTCAACCAAGGCCAGTGCTGTACAGCGGCCTCCAGGGTGTTCGTGGAAGAGCAGGTCTA
  CGGGGAGTTTGTGAGGAGGAGTGTGGAGTTCGCCAAGAAGAGGCCAGTTGGAGACCCCTTCGATGCCAAA
  ACGGAGCAGGGGCCTCAGATCGACCAAAAGCAGTTTGACAAAATCCTCGAGCTGATTGAGAGTGGGAAGA
  AGGAAGGGGCCAAGCTAGAATGTGGGGGGTCAGCCATGGAGGACAGAGGGCTGTTCATCAAACCCACGGT
  CTTCTCAGATGTTACGGACAACATGAGGATTGCCAAAGAGGAGATTTTCGGACCAGTGCAGCCGATCCTG
  AAGTTCAAAAACCTGGAGGAGGTGATCAAGAGAGCGAATAGCACCGACTATGGACTCACAGCAGCAGTGT
  TCACCAAAAACCTGGACAAAGCACTGAAGCTGGCTGCTGCGCTCGAGTCGGGGACAGTCTGGATCAACTG
  CTACAATGCATTTTATGCACAGGCTCCATTTGGTGGCTTCAAAATGTCTGGGAATGGCAGAGAACTAGGA
  GAATATGCTCTGGCTGAATATACAGAAGTGAAAACTGTCACCATCAAACTCGAGGAGAAGAACCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222097 representing NM_053080
  Red=Cloning site Green=Tags(s)
  
  MATTNGAVENGQPDGKPPALPRPIRNLEVKFTKIFINNDWHESKSGRKFATYNPSTLEKICEVEEGDKPD
  VDKAVEAAQAAFQRGSPWRRLDALSRGQLLHQLADLVERDRAILATLETMDTGKPFLHAFFVDLEGCIKT
  FRYFAGWADKIQGRTIPTDDNVVCFTRHEPIGVCGAITPWNFPLLMLAWKLAPALCCGNTVVLKPAEQTP
  LTALYLASLIKEVGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVREAASRSNLKRVTLE
  LGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASRVFVEEQVYGEFVRRSVEFAKKRPVGDPFDAK
  TEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAMEDRGLFIKPTVFSDVTDNMRIAKEEIFGPVQPIL
  KFKNLEEVIKRANSTDYGLTAAVFTKNLDKALKLAAALESGTVWINCYNAFYAQAPFGGFKMSGNGRELG
  EYALAEYTEVKTVTIKLEEKNP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053080
• ORF Size: 1536 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053080.3, NP_444310.3
• RefSeq Size: 3423 bp
• RefSeq ORF: 1539 bp
• Locus ID: 56847
• UniProt ID: Q9JHW9
• Cytogenetics: 7 C
• Gene Summary: NAD-dependent aldehyde dehydrogenase that catalyzes the formation of retinoic acid (PubMed:11044606, PubMed:11013254, PubMed:14623956). Has high activity with all-trans retinal, and has much lower in vitro activity with acetaldehyde (By similarity). Required for the biosynthesis of normal levels of retinoic acid in the embryonic ocular and nasal regions; retinoic acid is required for normal embryonic development of the eye and the nasal region (PubMed:14623956).[UniProtKB/Swiss-Prot Function]