Dnaaf5 (NM_001081265) Mouse Tagged ORF Clone

CAT#: MG221395

Heatr2 (tGFP-tagged) - Mouse HEAT repeat containing 2 (Heatr2), (10ug)

Product Images

Circular map for MG221395

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dnaaf5
• Synonyms: BC053401; C76907; Heatr2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221395 representing NM_001081265
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCGGCGGAGGCGGAGGTGGCCGCGGCCCCGGGCTTAACCGAGGCGGCTGAAGCCGCGGAAC
  TGACCCGCGCGCTAAGCCGCCTGCTACCGGGGCTGGAGACGGAAAGTAAGCTGGGGCGGCGGCGCGCTCT
  GGAGGCTCTGGAGCAGGTACTGGAAGAGGCGGTGCGGCCCGGCGCGGATTCCGCGGCCTTCCAGGGCCCC
  TGGGCCCGTCTGCTGCTGCCGCGCCTGCTGCGGCTTCTAAGCGATCCGGCCGAGGGCTGCCGCGCGCTGG
  CCGCTCACCTGCTCGACCTTGGCCTGCGCCGCGCCGCGCGGCCCCGCGACGCTCTGCCGCGTCTGCTGCC
  GGCGCTCAGCGCACGCCTGGCCCGACCTGAACTCGCGCGGCCGCCGCCGGAGCCCTGCGAGGAGCTGCGT
  CTCGCCCTCGTGCAGCTGCTCCACCTCGCCGTAGACCTGGGCGGCGCCGCGCTCGCGCCCCACCTGGACG
  ACGCCGTGCGCGCCTTGCGGGCCGCGCTGCTCGACCCCTTCGCTGCGGTGCGCCGGGAGGGCTGCGAGTG
  CGCAGCCGCTCTGGCCCGTGCCACTCCGGAGCACTTCCACATGCAATCGGAGTCCTTGATAGGCCCCCTG
  ATGCAGACCATCTCCCACCAGCACTGGAAGGTCCGGGTAGCCGTTATCGAAGCCACGGGCACTGTGATCC
  AGTTTGGCAGTGGGAACTCGGTGGATGATGTCCTTTCCCACTTTGCTCAAAGACTCTTCGATGACGTCCC
  TCAGGTCCGGCAGGCAGTGACCTCGGTGGTCGGGGGATGGCTGCTGAATCTCCGGGACCGCTACTCCTTC
  CTACACAAGCTCACCCCACTGCTGCTCAGCAGCTTCAGTGATGAAATGCCTGAGATCCGGCAGACAGCCA
  CCAGCCTATGGGAGAAGGTCGGGCTGCAGTGGCAGCAGGAGAACGAGGCAGATCTAAAGGACAAGCTGGA
  CTTCGCTTCACCTCCTCCTCCCAACTACCCTGAGCACGAGAGCCGCCCTGGCCTGGGCTGCCGAGAGCTG
  GTCTTCAGGAACCTCTCCAAGGTGCTTCCTGCCATCTGTCATGATATCACCGACTGGGTGGTGGGGACGC
  GGGTGAAGGCAGCACAGCTGCTGCCGGTGCTGCTGCTGCACGCAGAGGACCACATCACCCAGCACCTGGA
  GATCGTCCTCAGAACCCTGCATCAGGCCTGCACCGATGAAGAGAAGGCTGTGGTCGGCAGTTGCATAAGA
  GCCGCAGAGCTCATTGGCACCTTTGTCAGCCCAGAAGTATTTCTGAAACTGATCTTAGCCATGCTGAAGA
  AAGCACCCTCTGCCTCTGGCCTCTTGATCCTCGCTTCTGTCATCCGGGGCTGCCCCCGGAATGCCCTCCA
  GCCACACGTCACAGTCATAGCCACAGAGCTGGCCCAGGAGCACATCTGCCAGGGGTCTGAGAATAATCTC
  TACCTGGAGCATCTGCTGCTGTGTGTGCAGGCCCTGCTGTCTGTATGTCAGGAAGACTGTCGAGCTGCCA
  GCCTGCAGTTCCTGGAGGTGCTGGTGACAATAATGGCAGTCTCAGATGCCGTGGGTCTTGAGAAGAAGGC
  CCAGAAGACCATGGACACACTGGCTGAGGTGGAGGATATCCCCAGCAGTCAGGACCTCTACCGCAAGCAT
  GTGGGTGCACTCCTGGAGCGGCTGACAGCCTCGCATGGCGAGTGGGCAGTGCACTCTGTGCAGTTGCTGA
  AGTTCACTGTGCTCCTCACCCAGGCAGGCCCGGCTGTGGGAGAAGCGCTGCAGCATGTGATCCCCACCCT
  CAGGGCCTGTCTCCAGCCCTCCACAGACCCACACATGCGCCTGAAGCTCTTCTCTATCCTGTCCATGATG
  CTGCTGAGGCCTAAGGATACTGTCGATTCTCAGGGACAGTTCCGTGGCTATTTGGATATGGTAATAAATG
  ACATCCTAGCCCCCAACCTGCAGTGGCATGCAGGGAGGACAGCTGCAGCCATCCGGACGGCTGCTATATC
  CTGCCTGTGGGCACTCATCAGCAGTGACATCTTATCGGCCAAGCAGGTGCAAGAGGCACAAGAGACCCTG
  ATGCCACAAGTGCTGGCTACGCTGGAGGACGACTCCCAGACCACACGCCTGATGTCCTGCCGCATCATCA
  ACATGTTCCTCAAGAATTCAGGGGACACCATGGAGCCAGAGAAATTCCTCAAGGTCTATCCTGAGCTCTT
  GAAGCGCCTAGATGATGTCTCCAATGATGTGAGGATGGCAGCTGCTTCTGCCTTGCTCACCTGGCTCAAG
  TGCATCGAGAGCTTGGATGGGAAGTCCGCCTATCAGAGCAGCGTTCAGTTCCTGTACCGGGAGCTGCTGG
  TCCACCTGGATGACCCAGAGAGTGCCATTCAAGACACCGTCCTAGAAGTCCTCAAGGAGGGCAGCGTCCT
  GTTCCCAGATGTGCTGGTGCGGGAGACGGAGGCTGTCGTCCACAAACACCGCTCAGCTACCTACTGCGAG
  CAGCTGCTGCAGCACATGCAGACCATGGCCGCTGCTCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221395 representing NM_001081265
  Red=Cloning site Green=Tags(s)
  
  MAAPAEAEVAAAPGLTEAAEAAELTRALSRLLPGLETESKLGRRRALEALEQVLEEAVRPGADSAAFQGP
  WARLLLPRLLRLLSDPAEGCRALAAHLLDLGLRRAARPRDALPRLLPALSARLARPELARPPPEPCEELR
  LALVQLLHLAVDLGGAALAPHLDDAVRALRAALLDPFAAVRREGCECAAALARATPEHFHMQSESLIGPL
  MQTISHQHWKVRVAVIEATGTVIQFGSGNSVDDVLSHFAQRLFDDVPQVRQAVTSVVGGWLLNLRDRYSF
  LHKLTPLLLSSFSDEMPEIRQTATSLWEKVGLQWQQENEADLKDKLDFASPPPPNYPEHESRPGLGCREL
  VFRNLSKVLPAICHDITDWVVGTRVKAAQLLPVLLLHAEDHITQHLEIVLRTLHQACTDEEKAVVGSCIR
  AAELIGTFVSPEVFLKLILAMLKKAPSASGLLILASVIRGCPRNALQPHVTVIATELAQEHICQGSENNL
  YLEHLLLCVQALLSVCQEDCRAASLQFLEVLVTIMAVSDAVGLEKKAQKTMDTLAEVEDIPSSQDLYRKH
  VGALLERLTASHGEWAVHSVQLLKFTVLLTQAGPAVGEALQHVIPTLRACLQPSTDPHMRLKLFSILSMM
  LLRPKDTVDSQGQFRGYLDMVINDILAPNLQWHAGRTAAAIRTAAISCLWALISSDILSAKQVQEAQETL
  MPQVLATLEDDSQTTRLMSCRIINMFLKNSGDTMEPEKFLKVYPELLKRLDDVSNDVRMAAASALLTWLK
  CIESLDGKSAYQSSVQFLYRELLVHLDDPESAIQDTVLEVLKEGSVLFPDVLVRETEAVVHKHRSATYCE
  QLLQHMQTMAAAR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081265
• ORF Size: 2559 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081265.1, NP_001074734.1
• RefSeq Size: 3445 bp
• RefSeq ORF: 2562 bp
• Locus ID: 433956
• UniProt ID: B9EJR8
• Cytogenetics: 5 G2
• Gene Summary: Cytoplasmic protein involved in the delivery of the dynein machinery to the motile cilium. It is required for the assembly of the axonemal dynein inner and outer arms, two structures attached to the peripheral outer doublet A microtubule of the axoneme, that play a crucial role in cilium motility.[UniProtKB/Swiss-Prot Function]