F13a1 (NM_001166391) Mouse Tagged ORF Clone

CAT#: MG220954

F13a1 (tGFP-tagged) - Mouse coagulation factor XIII A1 subunit (F13a1) transcript variant 2, (10ug)

Product Images

Circular map for MG220954

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: F13a1
• Synonyms: 1200014I03Rik; AI462306; F13a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220954 representing NM_001166391
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGATACTCCAGCAAGCACCTTTGGGGGGAGGCGAGCAGTCCCGCCCAATAACTCCAATGCTGCAG
  AAGTGGACCTCCCAACTGAGGAGCTACAAGGCCTGGTGCCAAGGGGTGTCAACCTGAAAGATTACCTGAA
  TGTCACAGCTGTTCACCTGTTCAAGGAGAGATGGGACAGTAACAAGATTGATCACCACACAGACAAATAT
  GACAACAATAAGTTGATTGTCCGCAGAGGGCAGACCTTCTACATCCAGATTGACTTCAACCGTCCCTATG
  ACCCCAGGAAGGATCTCTTCAGAGTGGAATATGTCATTGGTCGCTACCCTCAGGAGAACAAGGGCACCTA
  CATCCCTGTGCCTGTAGTGAAAGAGCTGCAAAGCGGAAAGTGGGGAGCCAAGGTTATCATGAATGAGGAC
  CGGTCGGTGCGACTTTCCGTTCAGTCTTCTCCGGAATGCATTGTGGGGAAATTCCGCATGTATGTTGCAG
  TCTGGACTCCCTATGGCATCCTGCGTACTCGAAGAGACCCAGAAACAGACACATATATTCTCTTCAACCC
  TTGGTGTGAAGAGGACGCTGTGTATCTGGATGATGAGAAGGAAAGAGAAGAGTACGTCCTGAATGACATT
  GGAGTGATATTTTATGGGGACTTCAAAGACATCAAGAGCAGAAGCTGGAGCTATGGCCAGTTCGAAGACG
  GCATCCTGGATACTTGCTTGTATGTGATGGACAAAGCTGAGATGGACCTTTCTGGCAGAGGCAACCCCAT
  CAAAGTCAGCCGAGTTGGATCAGCAATGGTGAATGCCAAGGATGATGAAGGTGTTCTTGTTGGATCATGG
  GACAATGTCTATGCCTACGGCATCCCTCCATCAGCCTGGACAGGAAGTGTTGACATTCTACTAGAATACA
  GAAGCTCGGAAACACCAGTCCGATATGGCCAGTGTTGGGTTTTTGCTGGTGTCTTTAACACATTTTTAAG
  ATGCCTTGGAATCCCTGCACGAGTCATTACCAATTACTTCTCGGCCCACGACAATGATGCCAATCTGCAA
  ATGGACATCTTCCTGGAAGAAGATGGGAATGTGAGCTCCAAACTCACCAAGGATTCGGTGTGGAACTACC
  ACTGCTGGAACGAAGCGTGGATGACAAGGCCTGATCTTCCTGTTGGATTTGGAGGCTGGCAAGCTGTGGA
  CAGCACACCCCAGGAAAACAGTGATGGCATGTACCGCTGTGGCCCTGCCTCTGTCCAAGCCGTTAAGCAT
  GGCCATGTCTGCTTCCAGTTTGATGCCCCGTTTGTTTTTGCAGAGGTCAACAGTGATCTTGTTTACATCA
  CAGCTAAGCAAGATGGCACTCACGTGGTGGAAGCCGTGGATGCCACCCATATCGGGAAGCTAATTGTGAC
  CAAGCAAATTGGAGGAGATGGCATGCAGGATATCACGGATACTTACAAATTTCAAGAAGGCCAAGAAGAA
  GAGAGACTAGCCCTTGAAACTGCTCTGATGTATGGAGCCAAGAAGACCCTCAACACTGAAGGTGTTGTCA
  AATCAAGGTCTGATGTGACCATGAACTTTGATGTGGAAAATGCTGTGCTGGGAAAGGACTTCAAAGTGAC
  TATCACCTTCCAGAACAATAGCTCCAATCTGTACACCATCCTGGCCTATCTTTCTGGCAACATCACCTTC
  TACACTGGGGTATCCAAGAAAGAGTTCAAGAAGGAGTCCTTTGAAGAGACGCTGGATCCCTTTTCCTCCA
  AGAAAAAGGAGGTGCTGGTCAGAGCGGGCGAGTACATGAGCCACCTTCTAGAACAGGGCTTCTTGCACTT
  CTTCGTCACGGCACGCATCAACGAGTCCAGGGATGTCCTGGCCAAACAAAAGTCCATAATACTGACTATC
  CCCAAGATCACCATCAAGGTCCGAGGTGCTGCCATGGTTGGCTCTGATATGGTTGTGACTGTTGAGTTCA
  CTAATCCTTTAAAAGAAACACTACAAAATGTCTGGATTCATTTGGATGGTCCTGGAGTGATGAGACCCAA
  GAGAAAGGTGTTCCGTGAAATCCGGCCCAACACCACGGTGCAGTGGGAAGAAGTCTGTCGGCCTTGGGTC
  TCTGGCCATCGGAAGCTGATTGCCAGCATGACCAGTGACTCCCTGAGACATGTGTATGGAGAGCTGGATC
  TTCAGATTCAAAGACGACCTACTATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220954 representing NM_001166391
  Red=Cloning site Green=Tags(s)
  
  MSDTPASTFGGRRAVPPNNSNAAEVDLPTEELQGLVPRGVNLKDYLNVTAVHLFKERWDSNKIDHHTDKY
  DNNKLIVRRGQTFYIQIDFNRPYDPRKDLFRVEYVIGRYPQENKGTYIPVPVVKELQSGKWGAKVIMNED
  RSVRLSVQSSPECIVGKFRMYVAVWTPYGILRTRRDPETDTYILFNPWCEEDAVYLDDEKEREEYVLNDI
  GVIFYGDFKDIKSRSWSYGQFEDGILDTCLYVMDKAEMDLSGRGNPIKVSRVGSAMVNAKDDEGVLVGSW
  DNVYAYGIPPSAWTGSVDILLEYRSSETPVRYGQCWVFAGVFNTFLRCLGIPARVITNYFSAHDNDANLQ
  MDIFLEEDGNVSSKLTKDSVWNYHCWNEAWMTRPDLPVGFGGWQAVDSTPQENSDGMYRCGPASVQAVKH
  GHVCFQFDAPFVFAEVNSDLVYITAKQDGTHVVEAVDATHIGKLIVTKQIGGDGMQDITDTYKFQEGQEE
  ERLALETALMYGAKKTLNTEGVVKSRSDVTMNFDVENAVLGKDFKVTITFQNNSSNLYTILAYLSGNITF
  YTGVSKKEFKKESFEETLDPFSSKKKEVLVRAGEYMSHLLEQGFLHFFVTARINESRDVLAKQKSIILTI
  PKITIKVRGAAMVGSDMVVTVEFTNPLKETLQNVWIHLDGPGVMRPKRKVFREIRPNTTVQWEEVCRPWV
  SGHRKLIASMTSDSLRHVYGELDLQIQRRPTM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166391
• ORF Size: 2196 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166391.1, NP_001159863.1
• RefSeq Size: 3854 bp
• RefSeq ORF: 2199 bp
• Locus ID: 74145
• UniProt ID: Q8BH61
• Cytogenetics: 13 A3.3
• Gene Summary: This gene encodes subunit A of the coagulation factor XIII that catalyzes the final step of the blood coagulation pathway. The encoded protein associates with subunit B to form a heterotetrameric proenzyme that undergoes thrombin-mediated proteolysis to generate active factor XIIIa. The transglutaminase activity of factor XIIIa is required for the calcium-dependent crosslinking of fibrin, leading to the formation of a clot. Mice lacking the encoded protein display impaired reproduction and reduced survival due to bleeding episodes, hematothorax, hematoperitoneum and subcutaneous hemorrhage. Additionally, mice lacking the encoded protein exhibit impaired wound healing and inadequate healing of myocardial infarction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]