Cd33 (NM_001111058) Mouse Tagged ORF Clone

CAT#: MG219965

Cd33 (tGFP-tagged) - Mouse CD33 antigen (Cd33) transcript variant 1, (10ug)

Product Images

Circular map for MG219965

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cd33
• Synonyms: gp67; Siglec-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219965 representing NM_001111058
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGCCACTGCCGCTGTTCTTGCTGTGTGCAGGCTCCCTGGCTCAGGATTTAGAATTCCAGCTGG
  TGGCGCCCGAGTCAGTGACAGTCGAGGAGGGCCTATGTGTCCATGTGCCCTGCAGTGTTTTCTACCCCTC
  CATTAAGCTCACTTTAGGACCTGTGACCGGCTCCTGGCTCCGGAAAGGGGTCAGTCTCCATGAAGACTCT
  CCAGTGGCCACAAGTGACCCCAGACAACTAGTGCAGAAGGCAACACAGGGCAGATTCCAACTCCTTGGGG
  ACCCACAGAAACATGACTGTTCCCTGTTCATCAGAGATGCACAGAAAAATGACACAGGAATGTACTTCTT
  CAGAGTGGTCAGAGAACCTTTTGTGAGATATTCTTACAAAAAAAGCCAGCTGTCACTGCATGTGACCTCT
  CTATCACGGACTCCTGACATTATAATCCCGGGGACCCTGGAGGCTGGCTATCCTAGCAATCTCACCTGCT
  CTGTGCCCTGGGCTTGTGAGCAGGGGACACCCCCTACTTTCTCCTGGATGTCAACTGCCCTCACCTCCTT
  GAGTTCCCGAACCACAGACTCCTCCGTGCTGACGTTCACACCTCAGCCTCAGGACCATGGTACCAAACTC
  ACCTGCTTGGTGACCTTCTCTGGAGCAGGTGTCACTGTGGAAAGGACCATCCAGCTCAATGTTACCCGGA
  AATCAGGCCAGATGAGAGAGCTGGTCCTGGTGGCTGTGGGGGAGGCAACGGTCAAGCTCCTGATTCTTGG
  GCTCTGTCTCGTGTTTCTCATTGTGATGTTCTGCAGAAGGAAGACAACAAAGCTGTCAGTGCACATGGGC
  TGTGAAAATCCTATCAAGAGGCAGGAAGCGATCACATCCTATAATCACTGCCTATCTCCAACTGCATCTG
  ATGCTGTGACTCCAGGATGTTCCATACACAGGCTCATCAGCAGGACTCCAAGGTGCACAGCAATCCTGAG
  AATCCAAGACCCTTACAGAAGGACTCACCTCAGGAACAGAGCAGTGTCCACACTAAGATTTCCTTGGATT
  TCATGGGAGGGAAGCCTCAGGAGTACTCAGAGATCTAAATGCACCAAGCTTTGCTCTCCTGTGAAGAACC
  TGTGTCCTCTATGGCTTCCTGTGGATAATTCCTGCATCCCTCTGATCCCAGAGTGGGTTATGCTGCTGTG
  TGTGAGTCTTACACTCAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219965 representing NM_001111058
  Red=Cloning site Green=Tags(s)
  
  MLWPLPLFLLCAGSLAQDLEFQLVAPESVTVEEGLCVHVPCSVFYPSIKLTLGPVTGSWLRKGVSLHEDS
  PVATSDPRQLVQKATQGRFQLLGDPQKHDCSLFIRDAQKNDTGMYFFRVVREPFVRYSYKKSQLSLHVTS
  LSRTPDIIIPGTLEAGYPSNLTCSVPWACEQGTPPTFSWMSTALTSLSSRTTDSSVLTFTPQPQDHGTKL
  TCLVTFSGAGVTVERTIQLNVTRKSGQMRELVLVAVGEATVKLLILGLCLVFLIVMFCRRKTTKLSVHMG
  CENPIKRQEAITSYNHCLSPTASDAVTPGCSIHRLISRTPRCTAILRIQDPYRRTHLRNRAVSTLRFPWI
  SWEGSLRSTQRSKCTKLCSPVKNLCPLWLPVDNSCIPLIPEWVMLLCVSLTLS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001111058
• ORF Size: 1209 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001111058.1, NP_001104528.1
• RefSeq Size: 2571 bp
• RefSeq ORF: 1212 bp
• Locus ID: 12489
• UniProt ID: Q63994
• Cytogenetics: 7 28.25 cM
• Gene Summary: Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state (By similarity). Preferentially binds sialic acid to the short O-linked glycans of certain mucins (PubMed:12773563).[UniProtKB/Swiss-Prot Function]