A730008H23Rik (NM_172505) Mouse Tagged ORF Clone

CAT#: MG219847

A730008H23Rik (tGFP-tagged) - Mouse RIKEN cDNA A730008H23 gene (A730008H23Rik), (10ug)

Product Images

Circular map for MG219847

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: A730008H23Rik
• Synonyms: Fleg1; mFleg1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219847 representing NM_172505
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCTATGGGTCGGCAGGACCGCAGGCTGCAGCAGCAGCTTAATGAAAGCAGGAGCCGCTTCCAGA
  CGTTAATGAAGCGGCTGATAGCGAAGTACAACCAGCCCTTTGAGGACGACCCATTGGTGGAGATGACGAC
  GTTGACCTACGAGACGCCCCAAGGGTTGAGAGTTTGGGGAGGAAAGCTGATGAAGAAGGAAGACAAGGAA
  TACACTCAGAACGCAGAGAAGAGAGGTGGAAAAGACACAGTCATGACTTGGGTTCCTTCCGTGACCTCAT
  CTGTCACACCAGCCTCTGGATGCCAGGATGCTATCTCTGCAAAGAGCTCTGGTGGTCCTGGAGTATCTGC
  TTTGTCCTCTAGAGGCCAGGGTCCCTCATACCCTTGCCCAGCTGACATGGCCATTGTGGCTAGAAGTGAT
  GGCCTCTCCTTACTTGGGACCAGCAGCAACAGCGTAAGCAGCCAGTCCTTTGAGGTTGATGACCTTTGCA
  ATGTGACAATCAGCAATTTGTATGAAGGCATGATGCATTCCATGAGTAGGCTGCTGCGCTCAAAGCTATC
  CTGCATCATCTCCACCAAGACCTACATCAACCAGAGCTGGAAGCTCCGGAGGAGACCCTCACGCAAGCAG
  GGGCTGCACAAGAACAGGACACACTGCCCCCGGAGCAAGCCCTCCCAGAGGAGTGCCAGGAAGGGGCCTG
  CATCCTGCTCAGAGCACGGGAAAGAGGCCGGAATATTAAGAGACTACGGGAATTTACTGCACGTTGCTCC
  CCACAAGACAGGCTTAGAACTGAAAAGCGTTTCTCTTGAAGGAAGCAAACGCCAAGTCCATAAATTCAGT
  CCAGCTTGGAAGGAGCTCCAGATGATGCCGCAGAAGGACTTGGACTTAAACCGTGAGCGAGAAAATAGGG
  GGATGACATTACAGTGGCTAATTTCACCCGTCAAGGTGGTTCCCAGACCAAGGATGCTTCCAAGTCAAGT
  AGAGAAGTGGTATAGAGAGATTAAAATCAAATTTGACAAGCTTCATCAGGAATATTGCCTGTCTTCTGGG
  AAACAGCCTCGCCTGACTGACCCCACAGAGTCTTGGGCTGTCGATGTGTACAGAAGTGGTTCTAAGAGCC
  CAGGCAGCCGTCAGGATGTAGAAACCTGCAGGCCCAGTTCACCTTTCGGCAGAGAAAAGACAGAAAGGCC
  AGGTGAAGCTTTAGAAGACCTGAGGGGAAATGGAAAGTCTGTCAAAACAAAGAGCTGTCTTCTAAGGAGC
  TGTCCCTCTCCAGAGGGCAGCCCTTCACGGAGCCCCAGCCATTCTCAGCTGAGCTCTGGCCTTCAAGAGC
  ACAATTCTGAACCTACTGGTAAGGCAGTGTGGCCCAGTACAGCTATTTCAGCACCATGCATAGGCTCTCC
  AGGCTGTGGGAAGGATAACTATGATGAGCTTAAAAAAGAATTCAACAGACTTTATCAGAAGTATTGTCTG
  TCACCGCAGCGGGCGAAGGTGACTTCGTGTGGCAGAGTATCGCCAATGAAAGCTGCTGCAGCTCTTCCCT
  GTCAATCAGAGCACTTGAAGAGACTAAATCCAGACTCCCCACAACAGAGTTCCCAGAAACGGTCAATCTC
  TCCTGGCTGTCACAGAAGAGTCCTGCAGGATTCAACTCCACAGACTGCTAGTACACTTGTGAGAGAGCCT
  TGGCTTCCAACAAAGAGGCGCAAGTTGTCATACCCGGTAGCTTGTGCTCACCAGGCCAAATCTCACAATA
  CCTCAGGAGCATCAGGCTGGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219847 representing NM_172505
  Red=Cloning site Green=Tags(s)
  
  MESMGRQDRRLQQQLNESRSRFQTLMKRLIAKYNQPFEDDPLVEMTTLTYETPQGLRVWGGKLMKKEDKE
  YTQNAEKRGGKDTVMTWVPSVTSSVTPASGCQDAISAKSSGGPGVSALSSRGQGPSYPCPADMAIVARSD
  GLSLLGTSSNSVSSQSFEVDDLCNVTISNLYEGMMHSMSRLLRSKLSCIISTKTYINQSWKLRRRPSRKQ
  GLHKNRTHCPRSKPSQRSARKGPASCSEHGKEAGILRDYGNLLHVAPHKTGLELKSVSLEGSKRQVHKFS
  PAWKELQMMPQKDLDLNRERENRGMTLQWLISPVKVVPRPRMLPSQVEKWYREIKIKFDKLHQEYCLSSG
  KQPRLTDPTESWAVDVYRSGSKSPGSRQDVETCRPSSPFGREKTERPGEALEDLRGNGKSVKTKSCLLRS
  CPSPEGSPSRSPSHSQLSSGLQEHNSEPTGKAVWPSTAISAPCIGSPGCGKDNYDELKKEFNRLYQKYCL
  SPQRAKVTSCGRVSPMKAAAALPCQSEHLKRLNPDSPQQSSQKRSISPGCHRRVLQDSTPQTASTLVREP
  WLPTKRRKLSYPVACAHQAKSHNTSGASGWP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172505
• ORF Size: 1773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172505.4, NP_766093.1
• RefSeq Size: 2214 bp
• RefSeq ORF: 1776 bp
• Locus ID: 212427
• UniProt ID: Q6PG16