Lgr5 (NM_010195) Mouse Tagged ORF Clone

CAT#: MG219702

Lgr5 (tGFP-tagged) - Mouse leucine rich repeat containing G protein coupled receptor 5 (Lgr5), (10ug)

Product Images

Circular map for MG219702

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lgr5
• Synonyms: FEX; Gpr; Gpr49
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219702 representing NM_010195
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACCTCCTGCGTCCACATGCTCCTGTCCTTGCTGGCGCTGCTGCAGTTGGTGGCCGCCGGCAGCT
  CACCGGGACCAGATGCGATACCGCGGGGCTGCCCATCACACTGTCACTGTGAGCTGGATGGCAGGATGCT
  GCTCAGGGTGGACTGCTCGGACCTGGGGCTCTCGGAGCTGCCCTCCAACCTCAGCGTCTTCACCTCCTAC
  CTGGACCTCAGTATGAACAACATCAGTCAGCTACCCGCCAGTCTCCTACATCGCCTCTGCTTCCTAGAAG
  AGTTACGTCTTGCTGGAAATGCTTTGACACACATTCCCAAGGGAGCGTTCACGGGCCTTCACAGCCTCAA
  AGTGCTTATGCTGCAGAACAACCAGCTGAGACAGGTTCCGGAGGAAGCGCTACAGAATTTGAGAAGCCTT
  CAATCCCTGCGCCTAGATGCCAACCACATCAGCTACGTGCCACCCAGCTGTTTCAGCGGCCTGCACTCCC
  TGAGGCACCTGTGGCTAGATGACAATGCTCTCACAGACGTCCCTGTCCAGGCTTTCAGAAGTTTATCAGC
  CCTGCAAGCCATGACCTTGGCCCTGAACAAAATACACCACATAGCAGACTACGCCTTTGGAAACCTCTCC
  AGCCTCGTGGTTCTGCATCTCCATAATAATAGAATCCACTCCCTGGGAAAGAAATGCTTTGATGGACTCC
  ACAGCCTGGAGACTTTAGATTTAAATTATAATAACCTTGATGAATTCCCCACTGCAATCAAGACACTCTC
  CAACCTTAAGGAACTAGGATTCCACAGCAACAACATCAGGTCAATACCGGAGCGAGCGTTCGTAGGCAAC
  CCTTCTCTTATCACAATACACTTCTATGACAACCCCATCCAATTTGTTGGAGTATCTGCTTTTCAGCATT
  TGCCTGAACTAAGAACACTGACTTTGAATGGTGCCTCGCACATTACTGAATTTCCTCACTTGACAGGAAC
  TGCCACCCTGGAGAGTCTGACTTTAACTGGAGCAAAGATCTCATCTCTTCCCCAGGCCGTCTGTGATCAG
  TTACCTAATCTCCAAGTGCTAGATTTGTCTTACAACCTACTCGAAGACTTACCCAGTTTGTCAGGCTGCC
  AAAAACTTCAGAAAATTGACCTGAGGCATAACGAGATCTATGAAATTAAGGGCAGCACTTTTCAGCAGTT
  GTTTAACCTCCGATCTCTGAACTTAGCATGGAATAAAATTGCTATCATTCACCCCAATGCGTTTTCTACG
  TTGCCGTCTCTAATAAAGTTGGACCTATCATCCAATCTCCTGTCGTCCTTCCCTGTGACTGGGTTACATG
  GTTTAACTCACTTAAAATTAACAGGGAACCGAGCCTTACAGAGCCTGATACCATCTGCAAACTTCCCAGA
  GCTCAAGATTATAGAAATGCCATCTGCTTACCAGTGTTGTGCATTTGGGGGGTGTGAGAATGTCTATAAA
  ATTTCTAACCAATGGAATAAAGACGACGGCAACAGTGTGGACGACCTTCATAAGAAAGACGCTGGGTTAT
  TTCAAGTTCAAGATGAGCGGGACCTTGAAGATTTCCTACTTGACTTTGAGGAAGACCTGAAGGCCCTTCA
  CTCGGTGCAGTGCTCGCCTTCCCCAGGTCCCTTCAAGCCCTGTGAGCACCTATTTGGTAGCTGGCTGATC
  CGAATCGGGGTGTGGACCACGGCAGTACTGGCGCTTTCCTGCAATGCCTTGGTGGCTTTGACCGTGTTCA
  GAACTCCTCTGTACATCTCTTCCATAAAGCTGCTAATTGGGGTAATCGCGGTAGTGGACATTCTCATGGG
  GGTCTCCAGTGCTGTGCTGGCTGCCGTGGATGCATTCACTTTTGGCCGTTTTGCTCAGCACGGTGCGTGG
  TGGGAAGACGGAATCGGCTGCCAAATCGTTGGCTTCCTGTCCATTTTTGCTTCCGAATCGTCGATCTTCC
  TGCTCACTCTGGCAGCGCTGGAACGAGGTTTTTCTGTCAAGTGCTCTTCGAAGTTTGAAGTGAAAGCTCC
  CCTTTTTAGCCTGAGAGCGATCGTTTTGCTATGTGTCCTGTTGGCCCTGACCATTGCCACAATCCCCTTG
  CTAGGAGGCAGTAAGTACAATGCCTCTCCCCTCTGCCTGCCCTTGCCCTTTGGGGAGCCCAGCACCACGG
  GCTACATGGTGGCTCTCGTGTTGCTCAACTCTCTCTGTTTCCTCATAATGACCATTGCCTACACAAAGCT
  CTACTGCAGTTTGGAGAAAGGAGAGCTGGAGAATCTTTGGGATTGTTCGATGGTGAAGCACATTGCTCTG
  TTGCTCTTCGCCAACTGCATCCTTTACTGCCCCGTGGCTTTCTTATCCTTCTCCTCTTTGCTAAACCTCA
  CCTTTATCAGTCCTGACGTCATTAAATTTATACTTCTCGTGATCGTCCCACTTCCTTCCTGTCTCAACCC
  ACTTCTCTACATTGTCTTCAATCCCCATTTTAAGGAGGATATGGGCAGCCTGGGAAAGCATACCCGTTTC
  TGGATGAGATCAAAACACGCGAGTCTGCTGTCCATTAACTCGGACGATGTTGAGAAACGGTCCTGTGAGT
  CAACCCAAGCCTTAGTATCCTTTACCCACGCCAGCATAGCCTATGACTTGCCTTCCACTTCCGGGGCATC
  ACCAGCTTACCCCATGACTGAAAGCTGTCATCTCTCTTCAGTTGCATTTGTCCCATGTCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219702 representing NM_010195
  Red=Cloning site Green=Tags(s)
  
  MDTSCVHMLLSLLALLQLVAAGSSPGPDAIPRGCPSHCHCELDGRMLLRVDCSDLGLSELPSNLSVFTSY
  LDLSMNNISQLPASLLHRLCFLEELRLAGNALTHIPKGAFTGLHSLKVLMLQNNQLRQVPEEALQNLRSL
  QSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTDVPVQAFRSLSALQAMTLALNKIHHIADYAFGNLS
  SLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIKTLSNLKELGFHSNNIRSIPERAFVGN
  PSLITIHFYDNPIQFVGVSAFQHLPELRTLTLNGASHITEFPHLTGTATLESLTLTGAKISSLPQAVCDQ
  LPNLQVLDLSYNLLEDLPSLSGCQKLQKIDLRHNEIYEIKGSTFQQLFNLRSLNLAWNKIAIIHPNAFST
  LPSLIKLDLSSNLLSSFPVTGLHGLTHLKLTGNRALQSLIPSANFPELKIIEMPSAYQCCAFGGCENVYK
  ISNQWNKDDGNSVDDLHKKDAGLFQVQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLFGSWLI
  RIGVWTTAVLALSCNALVALTVFRTPLYISSIKLLIGVIAVVDILMGVSSAVLAAVDAFTFGRFAQHGAW
  WEDGIGCQIVGFLSIFASESSIFLLTLAALERGFSVKCSSKFEVKAPLFSLRAIVLLCVLLALTIATIPL
  LGGSKYNASPLCLPLPFGEPSTTGYMVALVLLNSLCFLIMTIAYTKLYCSLEKGELENLWDCSMVKHIAL
  LLFANCILYCPVAFLSFSSLLNLTFISPDVIKFILLVIVPLPSCLNPLLYIVFNPHFKEDMGSLGKHTRF
  WMRSKHASLLSINSDDVEKRSCESTQALVSFTHASIAYDLPSTSGASPAYPMTESCHLSSVAFVPCL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010195
• ORF Size: 2721 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010195.2, NP_034325.2
• RefSeq Size: 4711 bp
• RefSeq ORF: 2724 bp
• Locus ID: 14160
• UniProt ID: Q9Z1P4
• Cytogenetics: 10 D2
• Gene Summary: The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. [provided by RefSeq, Sep 2015]