Mapre2 (NM_001162941) Mouse Tagged ORF Clone

CAT#: MG219382

Mapre2 (tGFP-tagged) - Mouse microtubule-associated protein RP/EB family member 2 (Mapre2) transcript variant 2, (10ug)

Product Images

Circular map for MG219382

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mapre2
• Synonyms: AI314113; C820009F03Rik; D18Abb1e; EB1; EB2; RP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219382 representing NM_001162941
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAACAGATCAGAGATCAACGGGTAATCAGAAAGGGTCCCCAGAAGCACCATTCACTTCAACAGCCAG
  GGAGAGTGCCTGGATGTTCAAATTGGGGAATGGCGGTCAATGTGTATTCTACCTCGATAACCCAGGAGAC
  TATGAGCAGGCATGACATCATTGCATGGGTCAATGACATCGTGTCTCTAAACTACACAAAAGTGGAACAG
  CTCTGTTCAGGAGCAGCCTATTGCCAGTTCATGGACATGCTCTTCCCGGGCTGTATTAGTTTGAAGAAAG
  TAAAATTCCAAGCAAAGTTGGAGCATGAGTATATTCACAACTTTAAACTTCTGCAAGCATCCTTTAAACG
  GATGAATGTTGATAAGGTAATCCCAGTGGAGAAGCTGGTGAAAGGGCGTTTCCAAGACAACCTGGATTTT
  ATTCAGTGGTTTAAGAAGTTCTACGATGCTAACTATGACGGGAAGGAGTACGATCCTGTTGAGGCCCGAC
  AAGGGCAAGATGCAATTCCTCCCCCTGACCCTGGTGAACAGATCTTCAACCTGCCCAAGAAGTCGCACCA
  TGCAAACTCCCCCACAGCAGGCGCGGCTAAGTCAAGTCCTGCATCTAAGCCAGGATCCACACCTTCTCGC
  CCCTCGTCTGCCAAAAGGGCTTCATCCAGTGGCTCTGCATCCAGATCTGACAAAGATTTAGAAACGCAGG
  TCATACAGCTCAACGAGCAGGTACATTCATTGAAACTCGCCCTAGAAGGTGTGGAGAAGGAGAGGGATTT
  CTACTTCGGGAAGTTGAGAGAGATTGAGCTGCTGTGCCAAGAACATGGGCAGGAGAACGATGACCTCGTG
  CAGCGACTAATGGAAGTGCTCTACGCTTCCGATGAACAGGAGGGCCAAACGGAAGAGCCAGAAGCAGAGG
  AGCAAGCCCATGACCAGCAGCCCCAGCAGCAGGAGGAGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219382 representing NM_001162941
  Red=Cloning site Green=Tags(s)
  
  MKQIRDQRVIRKGPQKHHSLQQPGRVPGCSNWGMAVNVYSTSITQETMSRHDIIAWVNDIVSLNYTKVEQ
  LCSGAAYCQFMDMLFPGCISLKKVKFQAKLEHEYIHNFKLLQASFKRMNVDKVIPVEKLVKGRFQDNLDF
  IQWFKKFYDANYDGKEYDPVEARQGQDAIPPPDPGEQIFNLPKKSHHANSPTAGAAKSSPASKPGSTPSR
  PSSAKRASSSGSASRSDKDLETQVIQLNEQVHSLKLALEGVEKERDFYFGKLREIELLCQEHGQENDDLV
  QRLMEVLYASDEQEGQTEEPEAEEQAHDQQPQQQEEY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162941
• ORF Size: 951 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162941.1, NP_001156413.1
• RefSeq Size: 3993 bp
• RefSeq ORF: 954 bp
• Locus ID: 212307
• UniProt ID: Q8R001
• Cytogenetics: 18 12.08 cM
• Gene Summary: May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).[UniProtKB/Swiss-Prot Function]