Cnot4 (NM_001164411) Mouse Tagged ORF Clone

CAT#: MG219277

Cnot4 (tGFP-tagged) - Mouse CCR4-NOT transcription complex subunit 4 (Cnot4) transcript variant 3, (10ug)

Product Images

Circular map for MG219277

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cnot4
• Synonyms: Not4; Not4h; Not4hp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219277 representing NM_001164411
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGCAGTCCTGATGCAAAGGAAGACCCTGTGGAATGCCCTCTTTGCATGGAGCCCTTGGAAATAG
  ATGATATTAACTTTTTCCCTTGCACCTGTGGCTACCAGATATGCCGATTTTGTTGGCATCGAATTCGCAC
  TGATGAGAATGGGCTTTGTCCTGCATGTAGAAAACCATATCCAGAAGATCCAGCAGTTTACAAACCACTT
  TCCCAGGAAGAATTACAAAGGATAAAGAATGAGAAAAAACAGAAACAAAATGAAAGAAAACAGAAAATCT
  CAGAAAATAGAAAACATTTGGCTAGTGTACGTGTTGTACAAAAAAACCTTGTGTTTGTTGTAGGTCTGTC
  ACAGCGCCTAGCAGACCCAGAAGTTTTAAAACGACCAGAGTATTTTGGGAAGTTTGGTAAAATACATAAA
  GTTGTCATCAATAATAGCACATCCTATGCAGGCTCACAGGGTCCGAGTGCCAGTGCTTATGTAACGTATA
  TCCGGTCAGAAGATGCTCTCAGAGCAATACAGTGTGTCAACAATGTGGTAGTAGATGGCAGGACACTAAA
  GGCATCTTTAGGTACAACAAAGTACTGCAGTTATTTCTTAAAGAACATGCAGTGCCCAAAGCCTGACTGC
  ATGTATCTTCATGAATTGGGAGATGAGGCAGCCAGCTTCACAAAAGAGGAAATGCAGGCGGGTAAGCACC
  AAGAATATGAACAGAAGCTCCTCCAAGAATTATATAAATTAAATCCCAATTTTCTTCAGCTATCTACGGG
  TTCAGTTGATAAGAATAAGAACAAAGTGACACCATTACAAAGCCCCATTGACAAACCTTCAGATTCTCTC
  AGTATAGGGAATGGTGATAATTCCCAACAGATATCTAACAGTGATACGCCTTCACCACCACCTGGTTTAT
  CAAAATCCAACCCTGTCATCCCCATCAGTTCATCCAATCACAGTGCGAGGTCTCCTTTTGAAGGGGCAGT
  AACAGAGTCACAGTCATTATTCTCAGACAATTTTCGCCACCCCAACCCTATACCAAGTGGGCTTCCTCCT
  TTTCCCAGCTCTCCACAGACACCCAGTGATTGGCCTACAGCTCCAGAACCACAGAGCCTCTTCACATCAG
  AAACAATCCCAGTTTCATCATCTACAGACTGGCAAGCAGCATTCGGCTTTGGTTCTTCTAAACAACCAGA
  GGATGACTTGGGGTTTGACCCCTTTGATGTCACTCGAAAAGCCTTAGCAGACCTGATTGAGAAGGAACTA
  TCCGTCCAAGATCAACCTTCCCTTTCCCCCACATCTCTTCAGAACGCCTCCTCACACACTACGACCGCCA
  AAGGGCCAGGCTCTGGATTCCTGCACTCTGCTGCACCTACAAATGCCAACTCTCTCAATAGTACCTTTTC
  AGTCTTGCCACAGAGGTTCCCTCAATTTCAGCAGCACCGAGCAGTTTATAATTCGTTCGGTTTTCCAGGC
  CAGGCAGCCCGCTATCCTTGGATGGCCTTTCCACGCAATAGCATCATGCACTTGAACCACACAGCAAACC
  CCACCTCAAATAGTAATTTCTTGGACTTGAATCTCCCGCCACAGCACAACACAGGTCTGGGAGGGATCCC
  CATAGCAGGGGAAGAAGAGGTGAAGGTTTCGACCATGCCACTGTCCGCCTCTTCCCATTCATTACAACAA
  GGACAGCAGCCTACAAGTCTCCACACTACTGTGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219277 representing NM_001164411
  Red=Cloning site Green=Tags(s)
  
  MSRSPDAKEDPVECPLCMEPLEIDDINFFPCTCGYQICRFCWHRIRTDENGLCPACRKPYPEDPAVYKPL
  SQEELQRIKNEKKQKQNERKQKISENRKHLASVRVVQKNLVFVVGLSQRLADPEVLKRPEYFGKFGKIHK
  VVINNSTSYAGSQGPSASAYVTYIRSEDALRAIQCVNNVVVDGRTLKASLGTTKYCSYFLKNMQCPKPDC
  MYLHELGDEAASFTKEEMQAGKHQEYEQKLLQELYKLNPNFLQLSTGSVDKNKNKVTPLQSPIDKPSDSL
  SIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDNFRHPNPIPSGLPP
  FPSSPQTPSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKEL
  SVQDQPSLSPTSLQNASSHTTTAKGPGSGFLHSAAPTNANSLNSTFSVLPQRFPQFQQHRAVYNSFGFPG
  QAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPIAGEEEVKVSTMPLSASSHSLQQ
  GQQPTSLHTTVA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164411
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164411.1, NP_001157883.1
• RefSeq Size: 5613 bp
• RefSeq ORF: 1719 bp
• Locus ID: 53621
• UniProt ID: Q8BT14
• Cytogenetics: 6 B1
• Gene Summary: Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins. Involved in activation of the JAK/STAT pathway. Catalyzes ubiquitination of methylated RBM15.[UniProtKB/Swiss-Prot Function]