Ttll9 (NM_029064) Mouse Tagged ORF Clone

CAT#: MG218883

Ttll9 (tGFP-tagged) - Mouse tubulin tyrosine ligase-like family member 9 (Ttll9) transcript variant 2, (10ug)

Product Images

Circular map for MG218883

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ttll9
• Synonyms: 1700016F23Rik; 4930509O20Rik; AW046572
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218883 representing NM_029064
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGACAGAAGAATCAAAATTCCAAGGGCCATGGAGTGTCGAAAGGAAAAGAGAGAGAGCAGAGAA
  CCTTAATCCGCTTCAAGACCACCCTCATGAACACACTCATGGATGTTCTGCGCCACAGGCCCGGATGGGT
  GGAAGTCAAAGATGAAGGCGAATGGGATTTCTATTGGTGTGATGTCAGCTGGCTGCGGGAGAACTTTGAC
  CACACCTACATGGATGAGCACGTGCGCATAAGCCACTTCCGGAACCACTACGAGCTTACCAGGAAAAACT
  ACATGGTAAAGAACCTGAAGCGCTTCCGGAAGTACCTGGAGCGTGAGTCAGGAAAGACGGAGGCAGCCAA
  GTGTGACTTCTTCCCCAAAACCTTTGAGATGCCCTGTGAGTACCATCTGTTTGTGGAGGAGTTTCGCAAG
  AACCCCGGGATCACCTGGATCATGAAGCCTGTCGCCCGATCTCAGGGGAAGGGCATATTTCTCTTCCGGA
  GACTGAAAGACATCATGGACTGGAGGAAGGGCACTTCGGGGAAGAAACCCACCGGTGTGGAGACGCAGCC
  TGCTCGAGCCAACATGAACCCCTCAGGCAGCCATGACACAAGGAGTTCTGATGACCAGAAAGATGACCTC
  CCGGTGGAGAACTACGTGGCTCAGCGTTACGTGGAAAACCCCTACTTAATAGGAGGCCGCAAGTTTGACC
  TGCGCGTCTACGTGCTGGTGATGTCGTACATCCCTCTGCGGGCATGGCTGTACCGTGATGGCTTTGCCAG
  GTTCTCCAACACCCGATTCACACTGAATAGCATCGATGACCACTATGTTCACCTCACCAATGTTGCTGTG
  CAGAAGACATCGCCTGACTACCACCTAAAAAAGGGTTGCAAGTGGATGCTTCAGCGCTTCAGGCAGTACC
  TGGCTTCCAAGCATGGTCCTAAGGCCGTGGAGACACTCTTCAGTGACATGGACAATATCTTCATCAAGAG
  CCTGCAGAGCGTGCAGAAGGTGATCATCAGTGATAAGCACTGCTTTGAGCTCTATGGCTATGACATCCTC
  ATTGACCAGGACCTCAAGCCGTGGCTCCTAGAAGTCAATGCCTCTCCTTCCCTGACGGCCAGCAGCCAGG
  AGGACTATGAACTCAAGACCTGTCTGCTGGAGGATACCCTGCACGTGGTGGACATGGAGGCCAGGCTCAC
  AGGAAAGGAGAAGAGAGTCGGGGGCTTCGATCTCATGTGGAATGATGGCCCTGTCAGCCGAGAGGATGGG
  CCTTCTGACCTATCAGGGATGGGGAACTTTGTGACCAACACTCACCTAGGTACGTTGAGCCAGACAGGAA
  GTGAGTGGGAGAGAAACATCATAGGAAGTGGAGGGGGGCTGGGGCCGCTTGGCAGAGCAGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218883 representing NM_029064
  Red=Cloning site Green=Tags(s)
  
  MSRQKNQNSKGHGVSKGKEREQRTLIRFKTTLMNTLMDVLRHRPGWVEVKDEGEWDFYWCDVSWLRENFD
  HTYMDEHVRISHFRNHYELTRKNYMVKNLKRFRKYLERESGKTEAAKCDFFPKTFEMPCEYHLFVEEFRK
  NPGITWIMKPVARSQGKGIFLFRRLKDIMDWRKGTSGKKPTGVETQPARANMNPSGSHDTRSSDDQKDDL
  PVENYVAQRYVENPYLIGGRKFDLRVYVLVMSYIPLRAWLYRDGFARFSNTRFTLNSIDDHYVHLTNVAV
  QKTSPDYHLKKGCKWMLQRFRQYLASKHGPKAVETLFSDMDNIFIKSLQSVQKVIISDKHCFELYGYDIL
  IDQDLKPWLLEVNASPSLTASSQEDYELKTCLLEDTLHVVDMEARLTGKEKRVGGFDLMWNDGPVSREDG
  PSDLSGMGNFVTNTHLGTLSQTGSEWERNIIGSGGGLGPLGRAV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029064
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029064.2, NP_083340.2
• RefSeq Size: 1547 bp
• RefSeq ORF: 1395 bp
• Locus ID: 74711
• UniProt ID: A2APC3
• Cytogenetics: 2 H1
• Gene Summary: Probable tubulin polyglutamylase that forms polyglutamate side chains on tubulin. Probably acts when complexed with other proteins (By similarity).[UniProtKB/Swiss-Prot Function]