Hnrnpm (NM_001109913) Mouse Tagged ORF Clone

CAT#: MG218749

Hnrnpm (tGFP-tagged) - Mouse heterogeneous nuclear ribonucleoprotein M (Hnrnpm) transcript variant 2, (10ug)

Product Images

Circular map for MG218749

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hnrnpm
• Synonyms: 2610023M21Rik; AA409009; Hnrpm; mKIAA4193
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218749 representing NM_001109913
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGGGGTCGAAGCGGCAGCCGAAGTGGCGGCGACAGAACCCAAAATGGAGGAAGAGAGCGGCG
  CGCCCTGCGTGCCGAGCGGCAACGGAGCTCCGGGCCCGAAGGGTGAAGAACGACCTACTCAGAATGAGAA
  GAGGAAGGAGAAAAACATAAAAAGAGGAGGCAATCGCTTTGAGCCATATTCCAACCCAACTAAAAGATAC
  AGAGCCTTCATTACAAATATACCTTTTGATGTGAAATGGCAGTCACTTAAAGACCTGGTTAAAGAAAAAG
  TTGGTGAGGTAACATACGTGGAGCTCTTAATGGACGCTGAAGGAAAGTCAAGGGGATGTGCTGTTGTTGA
  ATTCAAGATGGAGGAGAGCATGAAAAAAGCTGCTGAAGTTCTAAACAAGCATAGTCTGAGTGGAAGGCCA
  CTGAAAGTCAAGGAAGATCCTGATGGTGAACATGCAAGGAGAGCAATGCAAAAGGCTGGAAGACTTGGAA
  GCACAGTATTTGTAGCAAATCTGGATTATAAAGTTGGCTGGAAGAAACTGAAGGAAGTATTTAGTATGGC
  TGGTGTGGTGGTCCGAGCAGACATTCTGGAAGATAAAGATGGGAAAAGTCGTGGAATAGGCATTGTGACT
  TTTGAACAGTCCATTGAAGCTGTGCAAGCAATATCTATGTTTAATGGCCAGTTGCTGTTTGATAGACCGA
  TGCACGTCAAGATGGATGAGAGGGCTTTACCAAAGGGAGACTTTTTTCCTCCTGAACGCCCACAGCAACT
  TCCCCATGGACTTGGTGGTATTGGCATGGGATTAGGACCTGGAGGTCAGCCTATTGATGCCAACCATCTG
  AGCAAAGGCATTGGAATGGGAAACCTAGGACCTGCAGGGATGGGAATGGAAGGCATAGGATTTGGAATAA
  ATAAAATTGGAGGCATGGAAGGACCCTTTGGTGGTGGTATGGAAAACATGGGCCGATTTGGATCCGGGAT
  GAACATGGGCCGAATAAATGAAATCCTAAGTAATGCACTGAAGAGAGGAGAGATCATTGCAAAACAGGGA
  GGAGGTGGAGCTGGAGGCAGTGTCCCTGGGATCGAGAGGATGGGCCCTGGCATTGACCGCATTAGCGGTG
  CTGGCATGGAGCGCATGGGCGCAGGCTTAGGCCATGGCATGGATCGAGTGGGCTCTGAGATTGAGCGCAT
  GGGCCTGGTCATGGACCGCATGGGCTCAGTTGAGCGCATGGGCTCCAGCATTGAGCGCATGGGCCCACTA
  GGCCTTGACCACATGGCCTCCAGTATTGAGCGCATGGGCCAGACCATGGAGCGCATTGGTTCTGGCGTGG
  AGCGCATGGGTGCCGGCATGGGCTTCGGCCTGGAGCGCATGGCCGCACCCATTGACCGTGTGGGCCAAAC
  CATTGAGCGCATGGGCTCTGGTGTAGAGCGCATGGGTCCTGCCATTGAACGCATGGGCCTAAGCATGGAT
  CGGATGGTGCCCACAGGCATGGGGGCCAGCCTGGAGCGCATGGGTCCTGTGATGGATCGGATGGCCACCG
  GCCTGGAGCGCATGGGCGCCAACAACCTGGAGCGCATGGGCCTGGAGCGTATGGGAGCCAACAGTCTTGA
  GCGCATGGGCCTGGAGCGAATGGGCGCCAACAGCCTGGAACGTATGGGCCCTGCCATGGGCCCAGCATTG
  GGCGCTGGTATTGAGCGAATGGGCCTGGCCATGGGTGGAGCCGGAGGTGCTAGCTTTGACCGAGCCATTG
  AGATGGAGCGGGGCAACTTTGGAGGAAGCTTCGCAGGTTCCTTTGGCGGAGCTGGAGGCCATGCACCTGG
  AGTAGCCAGGAAGGCCTGCCAGATATTTGTGAGAAATCTCCCATTTGATTTTACATGGAAGATGCTAAAG
  GACAAGTTCAATGAATGTGGCCACGTGCTGTACGCCGACATCAAGATGGAGAACGGAAAGTCCAAGGGGT
  GCGGTGTGGTTAAGTTTGAGTCTCCAGAGGTGGCTGAGAGAGCCTGCCGGATGATGAATGGCATGAAGCT
  GAGTGGCCGAGAGATTGATGTTCGAATTGATAGAAATGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218749 representing NM_001109913
  Red=Cloning site Green=Tags(s)
  
  MAAGVEAAAEVAATEPKMEEESGAPCVPSGNGAPGPKGEERPTQNEKRKEKNIKRGGNRFEPYSNPTKRY
  RAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRP
  LKVKEDPDGEHARRAMQKAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGIVT
  FEQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHL
  SKGIGMGNLGPAGMGMEGIGFGINKIGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQG
  GGGAGGSVPGIERMGPGIDRISGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSSIERMGPL
  GLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMD
  RMVPTGMGASLERMGPVMDRMATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPAL
  GAGIERMGLAMGGAGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLK
  DKFNECGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109913
• ORF Size: 2070 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109913.1, NP_001103383.1
• RefSeq Size: 2415 bp
• RefSeq ORF: 2073 bp
• Locus ID: 76936
• UniProt ID: Q9D0E1
• Cytogenetics: 17 B1
• Gene Summary: Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines (By similarity).[UniProtKB/Swiss-Prot Function]