Nudt18 (NM_153136) Mouse Tagged ORF Clone

CAT#: MG218249

Nudt18 (tGFP-tagged) - Mouse nudix (nucleoside diphosphate linked moiety X)-type motif 18 (Nudt18), (10ug)

Product Images

Circular map for MG218249

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nudt18
• Synonyms: BC036718
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218249 representing NM_153136
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGGAGGGCCTGGCAGGGGCGCTTGCCACCGTGCTGGGGGGAAAGGGGTTGCTGGTGCAGAGCT
  GTGACTCGGAGCCGGCTGGGAAGCCGCTGTTTCCCGTGCGGCTGCGGAAGAATGTCTGCTACGTGGTGCT
  GGCCGTGTTCCTCAACGAGCAGGATGAGGTGTTGATGATACAGGAGGCCAAGAGGGAATGCCGGGGGACA
  TGGTACCTCCCTGCAGGAAGAATGGAACCAGGAGAAACCATCGTGGAGGCCATGCAGCGGGAGGTGAAGG
  AGGAGGCAGGGCTGCTCTGTGAGCCAGTGACACTGCTGTCCGTGGAGGAGAGGGGCGCCTCCTGGATCCG
  CTTTGTGTTCCTCGCTCGACCAACAGGCGGAGTTCTCAAGACTTCCAAAGATGCTGATTCTGAGTCCCTC
  CAGGCCGGCTGGTACCCACGGGTCTCCCTGCCCACACCGCTTAGAGCCCATGATGTTCTGCACCTGGTAG
  AGCTAGGCGCCAAATTCTGCCAACAAGCCATGCACCCTCTCATTCTGCCTCAAGAGCTCCCCTGCAGTGT
  GGTCTGCCAACGGCTGGTGACCACCTTCACAACAGTCCAGTCGGTGTGGGTGTTGGTGGGCACGGTGGGG
  ACACCTCACTTGCCCATCACTGCCTGTGGCTTTACCCCTATGGAACAAAGGGGCGGCATCAAGGTGGCCA
  TCTTGAGGCTCCTACAGGAGTGTCTGACTCTGCACAGTTTGGCAGTGGAGACCAAGGGGTTGCTTGGGTT
  GCAACACCTAGGCAGAGACCATGTGGATGGTGTCTGCCTGAATGTGCTGGTGACCGTGGCTTTTCGGAAC
  CCAGGAATCCAAGACGAGCCCCCAAAGATTCGGGGTGAAAACTACTTTTGGTGGAAGGTATTAGAGGAAG
  ATTTACAAAAACTGCTTCTGTACAGACTCCAGGAATCTTCTGTGATCCCCCTGAGCAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218249 representing NM_153136
  Red=Cloning site Green=Tags(s)
  
  MATEGLAGALATVLGGKGLLVQSCDSEPAGKPLFPVRLRKNVCYVVLAVFLNEQDEVLMIQEAKRECRGT
  WYLPAGRMEPGETIVEAMQREVKEEAGLLCEPVTLLSVEERGASWIRFVFLARPTGGVLKTSKDADSESL
  QAGWYPRVSLPTPLRAHDVLHLVELGAKFCQQAMHPLILPQELPCSVVCQRLVTTFTTVQSVWVLVGTVG
  TPHLPITACGFTPMEQRGGIKVAILRLLQECLTLHSLAVETKGLLGLQHLGRDHVDGVCLNVLVTVAFRN
  PGIQDEPPKIRGENYFWWKVLEEDLQKLLLYRLQESSVIPLSR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153136
• ORF Size: 969 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153136.5
• RefSeq Size: 3716 bp
• RefSeq ORF: 972 bp
• Locus ID: 213484
• UniProt ID: Q3U2V3
• Cytogenetics: 14 D2
• Gene Summary: Mediates the hydrolyzis of oxidized nucleoside diphosphate derivatives. Hydrolyzes 8-oxo-7,8-dihydroguanine (8-oxo-Gua)-containing deoxyribo- and ribonucleoside diphosphates to the monophosphates. Hydrolyzes 8-oxo-dGDP and 8-oxo-GDP with the same efficiencies. Hydrolyzes also 8-OH-dADP and 2-OH-dADP. Exhibited no or minimal hydrolyzis activity against 8-oxo-dGTP, 8-oxo-GTP, dGTP, GTP, dGDP and GDP. Probably removes oxidized guanine nucleotides from both the DNA and RNA precursor pools (By similarity).[UniProtKB/Swiss-Prot Function]