Fhdc1 (NM_001033301) Mouse Tagged ORF Clone

CAT#: MG218209

Fhdc1 (tGFP-tagged) - Mouse FH2 domain containing 1 (Fhdc1), (10ug)

Product Images

Circular map for MG218209

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fhdc1
• Synonyms: 6330505N24Rik; Gm126
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218209 representing NM_001033301
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGTTATGAATTGTGTCTCCCTGGCCAGCGATAAAGAAAATGGGACTCTTGCCACAGCAGCTGCAT
  TCATGACTGGGCAGACGTCACCCTCTGCATCTCCTCCACCTCCTCCTCCACCGCCTCCACCTCCACCTTG
  TCCACATTCAGGGGAGGGCTTTTCCCCCTCCCCTCCTCCTCCTCTGCCACCTCCACTTCCTGGGGGACCT
  CCCATACCCCCTCCTCCTCCTCCAGGACTCCCCTCAGTTTCTTACCTGAATGGCTACAGCTCCCTGGGTA
  AGAAGAAACGAATGCGGAGCTTTTTCTGGAAAACCATTCCAGAGGAGCAAGTTCGAGGCAAGACCAACAT
  CTGGACCTTAGCAGCTAAGCAGCAGCATCAGTACCAGATCGACAAGAAGACCATTGAGGAGCTTTTTGGG
  CAGCAAGAAGACACCTCCAAGGCTTCTCTTCCCAAGAGAGGAGGAGCTTTGAATTCATCCTTCAGAGATG
  CCCGGGAAGAGGTTACCGTCTTGGATGCAAAGCGAAGTATGAACATTGGCATATTTCTTAAGCAGTTTAA
  AAAGTCTCCTCAGTCCATCGTAGAGGATATCTATCAAGGAAAGAGCGAGCATTACGGATCAGAGACGCTT
  CGAGAAATCCTTAAGCTTTTGCCGGAGTCAGAAGAGGTAAAGAAATTAAAGGCATTTAATGGCGATGTAT
  CCAAGCTCTCGCTGGCAGATTCCTTTCTGCACTGCTTGATTCAGGTGCCAAACTATTCACTTCGGATTGA
  AGCCATGGTGCTAAAAAAGGAGTTTTTGCCATCTTGTTCCTCTCTGTTCAAAGACATAAGGACTCTGAGA
  GCAGCTACAAAAGAGCTGATGTTATGTGAGGAGCTACATTCAATATTACACTTGGTGCTCCAGGCTGGGA
  ATATCATGAATGCCGGAGGGTATGCCGGCAATGCCGTAGGATTTAAACTGTCTTCTCTACTCAAATTGGC
  GGACACGAAGGCAAACAAACCTGGAATGAATCTTCTGCATTTTGTAGCTCAGGAAGCCCAGAAGCAAGAT
  GCCATCCTTCTAAACTTCTCTGAGAAGTTGCAGCACGTGCAAGAGACATCGAGATTATCTCTGGATATCA
  CCGAGGCAGAGCTGCACTCTCTCTTTGTCAGAACGAAATCGCTGCAGGAAAACATCCAGCTTGACCAGGA
  GCTGTGCCAGCAGATGGAAGACTTCCTCCAGTTTGCTGTGGAGAAGTTGGCGGAGCTGGAACTCTGGAAA
  CGGGAGCTGCAGGGTGAAGCTCACACCCTTATAGACTTTTTCTGCGAAGACAAAGAAACCATGAAACTGG
  ATGAATGCTTCCAGATATTCAGAGACTTCTGTACCAGATTCAACAAAGCAGTTAAGGACAATCATGACCG
  GGAGGAACAGGAGCGGAAGCAGCTACAGAGGCAGAAGGAGATGGAACAGAAGCGGTATTCCTGGTCAACT
  GGGGAACTCGGGAGCTTTGGCAGGAGCAGCAGTGAGAATGATGTGCAGATGCTAGCTAAGACAGGCACAG
  AAGACCTGCCCTCCTTCCTGAAGCCCAGGCCCAACAGCCCCTCCTACCGGCCCCCCAACACACGTCGCTC
  CCGCCTCTCCCTGGGCATCTCTGCGGACCGGGAGCTTTTGACCTTCCTGGAGAGTGCCACCAGCAGTCCA
  GAGGACCCCAACAAATTCAACAGTTTGCCCCGGAGCAGTCCCCGACAAGCTCGGCCCACCATCGCCTGGA
  TGGAACCCAGAGAACAACAGAGCCATGGCCCTAACTTTACACATGAACCTCAGGCCTCAAAGATCCAGGA
  GAAAGCCCCTCCCCCTGCTTGGCAAAATCAGCTTCCGACAACCTGGCGGGAGGAGCCTGCTTCTCCCCTC
  CCACTGGCTGGGCGGAGCAGGCCGAGCCTCCGAAAAAGGAACAGTGAACCTGTGGGCTTGGGTCCCACAC
  AAAGCCCTCCACTCTTACCATTGGATCTGGGTGTTAGGGAGCATGAGTTGGTCACAGGGCTGACCCAGTT
  TGACCTCCAGAGTCCCAAGAGCCTGGAGGAAGGATCCCAACTGACTCTGAATGACTTCTGTCCCACAAAG
  CTGCCATCTCCAGGGGATAGGAGCTCACAGCCCTTTGCTGCTGGCGGTGACAGTCTCCCACCCAAGGACA
  CAGACACCCAGGAGGTTCTCAGCCCAGCTGGGGAGGATGACAGGACCATCTCTGATGAACCCAGCAGTGA
  GGCTCTCGTGTCTGTGGTTGTCACCGACACTGAAGATAAGGATGCTGGACCTCTGTTATATGTCTCAGAT
  ACCACAGACTGCTCACTGACTCTGGACTGCTCCGAGGGAATGGACTCCAGAGCCGGGGGAGACAAGCAAG
  AGGAAGAGAAGGAAGGGGATGGCTCAGTGTCCTCTGGGGCTGGAGAGGCAGGAAGCAGCCAGGTTTCCTC
  CAACTCTGTTTCCAGCCCCCCTGGGGAGGTTCCTGCTCCAAAATCCAGTAAGAGTGAGCTGAGCTGCCAA
  GGTGGCCTTCCGAAGGACAGACCCAGCAGAGGGAAGGATGCTATAGCACCAAAGAGAAATTCCTTCAAAG
  AGGCTTCAGTAGGGGCTTCAAAGCCTGTCAGTGCACGGCGCAGCCAAGGGGTAACAACCAAGCCTGTGCG
  AACCTTGAACTCCTCCGAGAACGAACATATGCGCAAAGTGGTCCCTATCTCCAAGTCCAGCAGGGGAGCC
  GGGCCCTGGAAGCGGCCGGAACCGACACCCAAGGCTACCCCTCGGGAGACACCCAGCAGCACAGACACAC
  CACTATCACGAAGGAGCTCAGTGAGAGGAACTTCGGACACTTCGCCCAGGAGGCCCCAGGTGAGTGGATC
  TGGGGCAGAGGAGCCCAGGCTGCCCCGCTCGAGTGGCAGCATCAGTGGACGGCCAGGGAAGGACGCCCCG
  CTGCAGCCCAGAGCCTCGTTCAGGAAGCCTAGCGCCAAGCCCCTCAGAAACATCCCCAGGCAGAAACCTG
  AAGAAAACAAGGTCAGCAGCCCCAACTCTCCTGACCCCGAGAGTCCCAAGGAAGAGCCCAAGGCTCCTCA
  GGCTACGGGTGTCTCTCGAGCCCTTCCCCCCATCCCAAGTTTTGCTCGAAATACGGTGGCCTCGTCATCT
  CGGAGCCTGCGAACGGATGCTCCACCCGCGGCCAGAACCACTGGCCTCACCAGGACAGTCTCCCAGCGGC
  AGCTTAGGGTCAAAGGTGGCTCCGAGGATTCTGCCTCCAAGGACATTGGCACTTTGAAGAGGGCCAGCAG
  TGCTCGGGCCTCCAAGAAGTGTCCTGAATCTGCTGGGGGTTCAAGTGCCAATGTAGAAACCTCACTGAAG
  GGCAGAGGGACGACGGAAAGATCCTCTCTCAGACTGAAGGACTCGGGGCAGGCCACCCTCGGGAGGATCC
  TCCGTCCCCTGCAGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218209 representing NM_001033301
  Red=Cloning site Green=Tags(s)
  
  MHVMNCVSLASDKENGTLATAAAFMTGQTSPSASPPPPPPPPPPPPCPHSGEGFSPSPPPPLPPPLPGGP
  PIPPPPPPGLPSVSYLNGYSSLGKKKRMRSFFWKTIPEEQVRGKTNIWTLAAKQQHQYQIDKKTIEELFG
  QQEDTSKASLPKRGGALNSSFRDAREEVTVLDAKRSMNIGIFLKQFKKSPQSIVEDIYQGKSEHYGSETL
  REILKLLPESEEVKKLKAFNGDVSKLSLADSFLHCLIQVPNYSLRIEAMVLKKEFLPSCSSLFKDIRTLR
  AATKELMLCEELHSILHLVLQAGNIMNAGGYAGNAVGFKLSSLLKLADTKANKPGMNLLHFVAQEAQKQD
  AILLNFSEKLQHVQETSRLSLDITEAELHSLFVRTKSLQENIQLDQELCQQMEDFLQFAVEKLAELELWK
  RELQGEAHTLIDFFCEDKETMKLDECFQIFRDFCTRFNKAVKDNHDREEQERKQLQRQKEMEQKRYSWST
  GELGSFGRSSSENDVQMLAKTGTEDLPSFLKPRPNSPSYRPPNTRRSRLSLGISADRELLTFLESATSSP
  EDPNKFNSLPRSSPRQARPTIAWMEPREQQSHGPNFTHEPQASKIQEKAPPPAWQNQLPTTWREEPASPL
  PLAGRSRPSLRKRNSEPVGLGPTQSPPLLPLDLGVREHELVTGLTQFDLQSPKSLEEGSQLTLNDFCPTK
  LPSPGDRSSQPFAAGGDSLPPKDTDTQEVLSPAGEDDRTISDEPSSEALVSVVVTDTEDKDAGPLLYVSD
  TTDCSLTLDCSEGMDSRAGGDKQEEEKEGDGSVSSGAGEAGSSQVSSNSVSSPPGEVPAPKSSKSELSCQ
  GGLPKDRPSRGKDAIAPKRNSFKEASVGASKPVSARRSQGVTTKPVRTLNSSENEHMRKVVPISKSSRGA
  GPWKRPEPTPKATPRETPSSTDTPLSRRSSVRGTSDTSPRRPQVSGSGAEEPRLPRSSGSISGRPGKDAP
  LQPRASFRKPSAKPLRNIPRQKPEENKVSSPNSPDPESPKEEPKAPQATGVSRALPPIPSFARNTVASSS
  RSLRTDAPPAARTTGLTRTVSQRQLRVKGGSEDSASKDIGTLKRASSARASKKCPESAGGSSANVETSLK
  GRGTTERSSLRLKDSGQATLGRILRPLQK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033301
• ORF Size: 3447 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033301.4, NP_001028473.2
• RefSeq Size: 5930 bp
• RefSeq ORF: 3450 bp
• Locus ID: 229474
• UniProt ID: Q3ULZ2
• Cytogenetics: 3 F1
• Gene Summary: Microtubule-associated formin which regulates both actin and microtubule dynamics. Induces microtubule acetylation and stabilization and actin stress fiber formation (PubMed:18815276). Regulates Golgi ribbon formation (PubMed:26564798). Required for normal cilia assembly. Early in cilia assembly, may assist in the maturation and positioning of the centrosome/basal body, and once cilia assembly has initiated, may also promote cilia elongation by inhibiting disassembly (PubMed:29742020).[UniProtKB/Swiss-Prot Function]