Edem3 (NM_001039644) Mouse Tagged ORF Clone

CAT#: MG217624

Edem3 (tGFP-tagged) - Mouse ER degradation enhancer mannosidase alpha-like 3 (Edem3), (10ug)

Product Images

Circular map for MG217624

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Edem3
• Synonyms: 2310050N11Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217624 representing NM_001039644
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAAAGCCGGCGGCTGCCGGGGCTGTGGGTGCCGGGTCCCCCAGCGAGCGTCATGGAGCCTGGTGG
  CAGCGACGGCCGCGCTCTGTCTGGTGTTGGCCACGTCCGTGTGCACGGCAGGGGCCGCGCCCATGAGTAG
  GGAAGAGAAGCAGAAGCTTGGAAATCAAGTCCTGGAAATGTTTGATCATGCTTATGGCAACTATATGGAA
  CATGCCTACCCAGCTGATGAGCTCATGCCTTTAACCTGCCGGGGCCGTGTCAGAGGCCAGGAGCCCAGCC
  GGGGCGATGTGGATGACGCCTTGGGAAAGTTTTCCCTAACACTGATTGATTCTTTGGACACTCTTGTGGT
  ATTAAACAAAACTAAGGAATTTGAAGATGCTGTGAGAAAAGTTTTAAGGGATGTTAATTTAGATAATGAT
  GTAGTTGTTTCAGTCTTTGAAACAAACATCAGAGTTCTTGGGGGTCTTTTGGGCGGACACTCGCTGGCCA
  TCATGCTGAAGGAGAAAGGCGAGCACATGCAGTGGTACAATGACGAGCTCCTCCACATGGCAAAGCAGCT
  GGGTTACAAGCTCCTGCCAGCTTTCAACACCACCAGCGGCCTTCCGTACCCCAGGATTAATTTAAAGTTT
  GGCATCAGAAAACCAGAAGCCAGGACGGGAACAGAGACAGACACCTGTACAGCCTGTGCAGGCACCTTGA
  TCCTCGAGTTTGCTGCACTGAGTCGATTCACAGGAGCGACCATATTTGAGGAATATGCAAGAAAAGCTCT
  TGATTTTCTCTGGGAGAAAAGACAGCGAAGTAGCAATTTAGTAGGCGTGACTATCAATATTCATACTGGA
  GACTGGGTACGCAAAGATAGTGGAGTTGGAGCGGGGATCGACTCCTATTATGAGTATCTGTTGAAAGCCT
  ATGTCTTGCTTGGAGATGACAGTTTTCTGGAAAGATTTAATACACATTACGATGCCATAATGAGGTATAT
  AAGCCAGCCACCTCTCCTACTTGATGTGCATATCCACAAACCAATGCTGAACGCTCGGACCTGGATGGAT
  GCATTGCTTGCCTTTTTTCCAGGTTTGCAGGTTTTAAAGGGTGATATTAGACCTGCTATTGAAACTCACG
  AAATGTTATACCAGGTGATTAAAAAACACAATTTTCTACCAGAGGCATTTACTACAGATTTCAGGGTCCA
  TTGGGCTCAGCATCCTTTAAGGCCAGAGTTTGCAGAAAGTACCTACTTCTTATATAAGGCTACAGGAGAC
  CCTTACTACCTTGAAGTAGGGAAAACACTTATTGAAAATTTGAATAAGTATGCTCGAGTGCCTTGCGGGT
  TTGCTGCCATGAAGGATGTTCGCACTGGGAGTCATGAGGACAGAATGGATTCATTCTTCTTGGCTGAAAT
  GTTTAAATACCTGTACCTGTTATTTGCTGATAAAGAGGACATCATTTTTGACATAGAAGACTATATCTTT
  ACAACAGAAGCTCATCTGTTACCGCTTTGGCTTTCCACCACAAACCGAAGCATCTCAAAGAAGAACACAA
  CCTCAGAATACACAGAACTGGATGATAGTAATTTTGATTGGACTTGTCCAAACACTCAGATTCTTTTTCC
  TAATGATCCATTGTATGCTCAGAGCATCCGTGAACCCTTGAAAAACGTGGTGGATAAGAGCTGTCCGAGA
  GGCATCATCAGAGTAGAGGAGAGTTTCAGAAGTGGAGCTAAGCCTCCTCTGAGAGCCAGAGATTTTATGG
  CCACTAACCCTGAACATTTAGAAATCCTGAAGAAGATGGGGGTGAGTTTGATTCACCTCAAAGATGGGCG
  AGTCCAGCTGGTCCAACATGCAATCCAAGCTGCTAGTTCAATTGATGCTGAAGATGGGTTACGGTTCATG
  CAGGAGATGATAGAGCTGTCCAGTCAGCAACAGAAGGAGCAGCAGCTGCCTCCACGAGCTGTCCAGATTA
  TCTCCCACCCGTTTTTTGGCAGGGTCGTCTTAACTGCTGGACCAGCTCAGTTTGGGCTGGATCTATCTAA
  ACACAAAGAGACACGAGGATTTGTTGCAAGCAGTAAACCATATAATGGTTGTTCAGAGCTCACTAACCCT
  GAGGCAGTGATGGGAAAAATTGCCTTGATACAAAGAGGACAGTGCATGTTTGCAGAAAAGGCACGCAACA
  TTCAGAATGCTGGCGCCATCGGTGGCATTGTGATCGATGACAACGAGGGGAGCAGTAGCGACACGGCCCC
  CCTGTTCCAGATGGCCGGCGATGGGAAGGACACAGACGACATCAAGATCCCCATGCTGTTCCTGTTCAGC
  AAGGAAGGCAGCATCATCCTGGACGCCATCCGCGAGCACGAGCAGGTGGAGGTGCTCCTTTCTGACAAAG
  CGAGAGACCGAGATCCCGAAATGGAAAATGAAGACCAGCCATCTTCTGAAAATGATTCTCAGAATCAGAG
  CGCAGAGCAGATGCTCTCGCTTTCTCAGACAGTCGACTTGGCTGATAAGGAGAGCCCTGAACACCCTGCA
  GATTCTCACTCAGAGGCGTCTCCATCGGACAGTGAAGAGGCTGCTGGCTTTGCCCCCTCTGAACAGATAT
  CTGGGTCCACCGAAAACCACGAGACTACAAGCCTTGATGGTGAATGTACAGACTTAGATAACCAAGTTCA
  AGAACAATCAGAAACTGAGGAAGATTCCAGTCCTAATGTCAGCTGGGGTACAAAGGCCCAGCCTATAGAC
  TCCATATTAGCAGACTGGAATGAAGACATAGAAGCATTTGAAATGATGGAGAAGGATGAACTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217624 representing NM_001039644
  Red=Cloning site Green=Tags(s)
  
  MSKAGGCRGCGCRVPQRASWSLVAATAALCLVLATSVCTAGAAPMSREEKQKLGNQVLEMFDHAYGNYME
  HAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDND
  VVVSVFETNIRVLGGLLGGHSLAIMLKEKGEHMQWYNDELLHMAKQLGYKLLPAFNTTSGLPYPRINLKF
  GIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWEKRQRSSNLVGVTINIHTG
  DWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMD
  ALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGD
  PYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIF
  TTEAHLLPLWLSTTNRSISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPR
  GIIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSIDAEDGLRFM
  QEMIELSSQQQKEQQLPPRAVQIISHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPYNGCSELTNP
  EAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFS
  KEGSIILDAIREHEQVEVLLSDKARDRDPEMENEDQPSSENDSQNQSAEQMLSLSQTVDLADKESPEHPA
  DSHSEASPSDSEEAAGFAPSEQISGSTENHETTSLDGECTDLDNQVQEQSETEEDSSPNVSWGTKAQPID
  SILADWNEDIEAFEMMEKDEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039644
• ORF Size: 2793 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039644.2, NP_001034733.2
• RefSeq Size: 6604 bp
• RefSeq ORF: 2796 bp
• Locus ID: 66967
• UniProt ID: Q2HXL6
• Cytogenetics: 1 G1-G2
• Gene Summary: Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes, by catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2 in the N-glycans. Seems to have alpha 1,2-mannosidase activity.[UniProtKB/Swiss-Prot Function]