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Cep250 (NM_008383) Mouse Tagged ORF Clone
CAT#: MG217223
- TrueORF®
Cep250 (tGFP-tagged) - Mouse centrosomal protein 250 (Cep250) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_008383" in other vectors (2)
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Frequently bought together (4)
Other products for "Cep250"
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | Cep250 |
| Synonyms | AW490617; B230210E21Rik; Cep2; Inmp |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217223 representing NM_008383
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACAGGGAGCCCCGGGTTGAACATGAAGCCCCAGTCCTTGCAGCTGGTGCTGGAGGGGCAGGTGC TGGCGCTGCAGCAGCAGATGGCCGAGAACCAGGCTGCCTCCTGGCGGAAACTGAAGAACTCCCAAGAGGC CCAGAAGAGACAAGCAACCCTCGTGAGGAAGCTGCAGGCCAAGGTTTTACAGTACCGGAGCTGGTGTCAA GACCTGGAGAAGCGACTAGAAGCCACTGGGGGGCTGATCCCTCAGCGCTGGGAGAGTGTAGAAGAGCCCA ACCTGGAACAGTTGCTGATCCGATTGGAGGAGGAGCAGCAGAGGTGTGAGAGTCTAGTGGAGGTGAACAC CGAGCTTCGGCTGCACATGGAGAAAGCCGATGTGGTGAATAAAGCCTTGCAGGAGGACGTGGAAAAACTG ACAGTGGACTGGAGTCGGGCCCGAGATGAGTTAGTGAGGAAGGAGAGCCAGTGGCGGATGGAGCAGGAGT TCTTCAAGGGCTATTTAAGAGGTGAACACGGTCGTCTTCTCAATCTATGGCGAGAGGTGGTAACCTTCCG ACGTCACTTCCTGAAAATGAAGTCAGCTACTGACAGAGACCTGACAGAGCTAAAGGCTGAACACGCAAGG CTTTCAGGGTCCCTGCTGACCTGCTGTCTGCGCCTGACCCTGAGAGCACAGTCTCGGGAGTCCAGTGGAT CTGGGAGAACGGAGGAGAGTGAGCCAGCCCGGCTGCTGCTGCTCGTCGCTAAGACCCAGGCGCTGGAGAA GGAAGCCCATGAGAAGAGCCAGGAGTTAATGCAGCTCAAGAGTCACGGGGATCTAGAGAAGGCAGAGCTG CAGGACCGAGTGACGGAACTCTCTGCTCTGCTAACCCAGTCTCAGAAGCAAAATGAAGATTATGAGAAGA TGGTAAAGGCTCTAAGAGAAACAATGGAGATCCTGGAGACAAACCATGCAGAGTTAATGGAGCATGAGGC ATCTCTCAGTAGGAATGCTCAAGAGGAAAAGTTGTCTCTGCAGCAGGTGATCAAGGCTATAACCCAGGCA CTGGCCTCAGTGGAAGAAGAAGACACTGTGACCCAGTCCTCAGGCCATGAGGACTTGCTGCAGTCAGACT GTAATGGCCTTTCCCAGTTTGATCCCCAGGACCCAGACAGGGCTCTTACCCTGGTGCAGTCAGTGCTGAC TCGGAGACAGCAGGCTGTGCAGGACCTCAGGCAGCAGCTTTCAGGCTGCCAGGAGGCTATGAGCTTCTTG CAGCAGCAGCGCGATCAGTGGGAGGAAGAGGGCAGGGCCCTGAGGGAAAGGCTGCAGAAGCTCACTGGGG AGCGGGATGCTCTGGCAGGCCAGACTGTGGACCTTCAGGGAGAGGTGGACTCTCTCAGCAGGGAGCGGGA GCTGCTTCAGAAGGCCAGAGGAGAGCTGCAGCAGCAACTGGAAGTGCTGGAGCAGGAAGCGTGGCGACTG CGGAGGATGAACATGGAGCTGCAGCTGCAGGGGGACTCAGCTCAAGGGGAGAGGCTGGAGCAGCAGGAGG AGCTGCACTTGGCTGTCCGGGAGAGGGAGCGCCTTCAGGAGACGCTGGTGGGCCTGGAAGCCAAGCAGTC AGAATCACTAAGTGAGCTGCTCACTCTTCGAGAAGCCCTGGAGTCAAGTCGCCTAGAAGGGGAGTTACTG AAGCAGGAGCGAGTGGAAGTGGCTGCAGCCTTGGCCAGGGCAGAACAGTCCATTGTGGAGCTGTCAGGTT CTGAGAACAGCCTGAAGGCAGAGGTAGCTGATCTTCGAGCTGCAGCTGTCAAGCTTGGGGCCTTAAATGA GGCTTTGGCTTTAGATAAAGTTGGACTGAACCAGCAGCTTCTCCAGTTGGAGCAGGAGAACCAGTCTCTG TGCAGCAGAGTGGAGGCCGCAGAGCAGCTGAGAAGCGCTTTGCGGGTGGACCTGGCGGAGGCGGAAAGGC GCAGGGAAGCCCTGTGGGAGAAGAAAACTCAGCTGGAGACTCAGCTGCAGAAGGCAGAGGAAGCGGGGGC TGAGCTACAGGCAGAGCTGAGGGGCACCCGAGAAGAAAAGGAAGAGCTTAAAGATAAATTAAGTGAGGCA CATCACCAGCAAGAAACAGCTACAGCTCACCTAGAACAGCTGCATCAGGACGCAGAAAGACAGGAAGAAA CACTTGCTAGGGCAGTCCAGGAGAAGGAAGCCCTAGTACGGGAGAGGGCAGCTCTTGAGGTGCGCCTGCA GGCTGTGGAGCGGGACCGACAGGACCTCACTGAACACGTCCTGGGGCTCAGGTCAGCCAAGGAGCAACTG GAGAGCAATCTGTTTGAGGCCCAACAGCAAAATTCTGTGATACAGGTCACCAAGGGGCAGCTGGAGGTCC AGATTCAAACTATTATCCAAGCCAAGGAAGTAATTCAAGGGGAAGTGAAATGCCTGAAGCTGGAACTGGA TGCTGAGAGGACCCGGGCAGAGCAGGAGCGGGATGCAGTAGCCAGGCAGCTAGCCCAAGCTGAGCAGGAG GGGCAAGCATCTCTGGAGCGACAGAAGGTGGCCCACGAGGAGGAGGTGAACAGGCTGCAAGAGAAATGGG AGAAAGAGCGCTCCTGGCTGCAGCAGGAGCTGGATAAGACCCTGGAAACCCTCGAGAGGGAGAGAGCAGA GCTGGAGACAAAGCTGAGAGAGCAGCAGACAGAAATGGAAGCCATCCGTACACAGAGGGAAGAGGAACGA AGCCAGGCTGACAGCGCCCTGTATCAAATGCAGCTAGAAACAGAGAAGGAGAGAGTATCCCTCCTGGAGA CACTGCTGAGGACCCAGAAGGAGCTGGCAGATGCTAGTCAACAGCTGGAAAGGCTGAGACAGGACATGAA GATTCAGAAGTTAAAGGAACAGGAGACCACTGGCATGCTGCAGGCCCAGCTTCAGGAAACACAGCAGGAG CTGAAGGAGGCAGCCCAGCAGCACAGGGACGACCTTGCTGCCTTCCAGAAGGACAAGCTGGACCTGCAGA AGCAGGTAGAGGACCTAATGTCTCAGCTGGTAGCCCACGATGACTCCCAGAGGCTGGTGAAAGAAGAGAT TGAAGAGAAGGTGAAAGTGGCTCAAGAATGCAGCCGGATTCAGAAGGAACTGGAGAAAGAGAACGCCAGC CTGGCTCTGTCGCTGGTGGAAAAGGAAAAACGACTTCTCATTTTACAAGAAGCTTACTCTGTAAGACAGC AGGAGCTGAGCTCCTTGCGCCAGGACATACAGGAGGCCCAGGAAGGGCAAAGAGAGCTGGGTGTACAGGT GGAACTATTGAGGCAGGAGGTGAAGGAAAAGGAGGCTGACTTTGTGGCCCGGGAAGCACAGCTGCTGGAG GAGTTGGAGGCCTCACGGGTTGCAGAGCAGCAACTTCGAGCGTCTTTGTGGGCCCAGGAAGCCAAGGCAA CCCAGCTGCAGCTGCAGCTGCGCAGCACTGAGAGCCAGCTAGAGGCATTGGTTGCAGAACAACAGCCGGA GAACCAGGCACAGGCACAGCTGGCTAGCCTCTGCTCTGTTCTGCAGCAGGCCCTGGGGTCTGCATGTGAG AGTAGGCCTGAGCTGAGGGGTGGAGGCGACTCTGCTCCTACCTTATGGGGCCCTGATCCAGATCAGAATG GAGCTTCCAGACTCTTTAAGAGAGGGTCGCTCCCAACGGCACTGTCACCGGAGGCTGTGGCACTAGCTCT CCAGAAGCTCCATCAGGACGTGTGGAAGGCTCGGCAGGCCCGGGATGACCTAAGGGACCAGGTCCAGAAG CTGGTGCAGCGCCTAACTGACACGGAGGCCCAGAAGAGCCAGGTTCACTCGGAGCTGCAGGACCTGCAGA GACAGCTCTCTCAGAGCCAGGAAGAGAAATCCAAGTGGGAAGGGAGGCAGAACTCCCTGGAGTCTGAGCT GAGGGACTTGCACGAGACCGCGGCATCCTTACAGAGTCGCCTCCGGCAGGCAGAACTGCAAAAAATGGAA GCTCAGAATGATCGAGAGTTGCTTCAGGCATCCAAGGAGAAGCTGTCAGCCCAGGTTGAACATCTACAAG CATGTGTTGCAGAAGCCCAGGCTCAGGCAGGTGCAGCTCCTGTCCTGGAAGAAGATCTGCGAACTGCTCG CTCTGCACTGAAACTGAAAAACGAGGAGCTAGAGAGTGAACGGGAGAGAGCCCAGGCTTTACAAGAACAG GGTGAGCTGAAGGTGGCTCAGGGGAAAGCTTTGCAGGAGAACCTAGCCCTGCTGGCACAGACCCTGTCTA ACAGAGAGCGGGAGGTCGAGACGTTGCAGGCTGAGGTTCAGGAACTGGAGAAGCAGCGGGAGATGCAGAA GGCTGCTCTGGAGCTGCTCTCCCTCGACCTGAAGAAGCGCAGTCGGGAGGTAGACCTGCAACAAGAACAG ATTCAGGAGCTGGAGCAGTGCAGGTCAGTCTTAGAGCACTTGCCCATGGCCGTCCAAGAGCGAGAGCAGA AACTGAGTGTGCAGCGGGACCAGATCAGGGAACTGGAGAACGATCGGGAGGCCCAGAGGAGTGTCCTGGA ACACCAGCTTCTGGACCTGGAGCAGAAGGCTCAAGTGATTGAGTCCCAGAGGGGACAGATTCAGGATCTG AAAAAGCAACTGGGGACACTGGAATGCCTGGCCCTGGAACTGGAGGAAAGCCATCACAAAGTGGAGAGCC AACAGAAGATGATCACAGAACTAGAGGGCCAGAGGGAAATACAAAGGGTGGCTCTAACCCACCTCACACT GGACCTGGAAGAAAGGAGCCAGGAGCTGCAGGCACAAAGCAGCCAGCTCCATGAGCTGGAGAACCATAGC ACCCATCTGGCAAAAGAGCTGCAGGAGAGGGACCAGGAGGTGACGTCCCAACGCCAGCAGATCGATGAGC TGCAGAAGCAGCAGGAACAGCTGGCTCAGGCCCTGGAGAGGAAAGGCCAGGAGCTGGTGCTGCAGAAGGA AAGGATTCAGGTTCTGGAAGATCAGAGGACGCTGCAGACCAAGATCTTAGAGGAGGACCTAGAACAGATC AAGCATTCCTTGAGAGAACGCAGCCAGGAGCTGGCCTCTCAGCGGCAGCTGGTGCATGAGCGTGCCGACG ATGGAAAGAGCCCGAGTAAAGGCCAGCGAGGGAGCCTGGAGCACTTGAAGCTGATCCTGCGTGATAAGGA GAAGGAGGTAGAGTGCCAGCAGGAGCGCATCCAGGAACTTCAGGGCCACATGGGCCAGCTGGAACAGCAG CTGCAGGGTCTGCACAGGAAGGTGGGGGAGACTAGCCTGCTCCTGACCCATCGAGAGCAGGAGACAGCAA CCCTGCAGCAGCATCTCCAGGAAGCCAAGGAACAAGGGGAACTGAGAGAGCAGGTACTTCAGGGTCAGCT GGAGGAGGCCCAGAGAGATCTGGCCCAGAGGGACCATGAACTAGAGACCCTCCGGCAGGAAAAACAGCAG ACCCAGGACCAGGAGGAAAGCATGAAGCTAAAGACAAGTGCTCTGCAGGCCGCTCTGGAGCAGGCCCATG CGACACTGAAGGAGCGTCAGGGGGAGCTGGAAGAACACAGGGAACAGGTGCGAAGGCTCCAGGAAGAACT AGAAGTGGAGGGACGGCAGGTACGGGCCCTGGAGGAGGTGTTGGGGGACCTAAGGGCTGAGTCTCGGGAG CACGAGAAGGCTGTGCTGGCCCTTCAACAACGGTGTGCTGAGCAGGCCCAGGAACATGAGGCAGAAGCCA GGACCCTGCAGGACAGCTGGCTGCAGGCCCAGGCCACTCTCACAGAACAAGAACAAGAGCTGGCGGCACT GCGAGCGGAAAACCAGTATTCCCGCCGACAGGAAGAGGCTGCAGTTAGCCAGGCAGAGGCGCTGCAGGAG GCCCTCAGCAAAGCCCAGGCTGCCCTGCAGGAGAAAGAGCAGAGTCTGCTTGAACAGGCTGAGTTGAGCC ACACCCTGGAGGCCAGCACTGCCGCCTTGCAAGCTACCCTGGACACCTGCCAGGCACGTGCCCGGCAGCT GGAGGAGGCCCTGAGGATTCGGGAAGGTGAGATCCAGGCCCAGGCTCTCCAACACCACGAGGTCACGCAG CACCTCCAGCAGGAACTTTGCCAGAAGGAAGAAGAGCTGAGGCAGCTACTAGAGAAGGCTGGGGCCCGGA GGAGTCAAGAAAATGGAATCCAAGAGAAGCAAAGTCTGGAGCAAGAGAGACAAGAGGAAACAAGGAGGCT CCTTGAGAGCCTAAAGGAGCTGCAGCTGACTGTAGCCCAAAGGGAAGAAGAGATTCTGATGCTGAGGGAG GCCTCGTCCCCAAGCCACAGAGCCCTCCCAGCCGAGAAGCCAGCTCTACAACCGCTCCCAGCACAACAGG AGTTAGAGCGGCTGCAGACTGCCCTAAGGCAAACAGAGGCCAGGGAGATTGAATGGAGGGAGAAGGCTCA GGACCTGGCTCTGTCCCTGGCCCAAAGCAAGGCCAGCATCAGCAGTCTGCAGGAGATAACCATGTTCCTA CAAGCCTCTGTTCTAGAGCGGGAATCAGAACAACAAAGACTACAGGAGGAGTTGGTACTCAGCAGACAAG CTCTGGAAGAGCAGCAGTCAGGTGGCCCACACTCAACCAGCAGAGCAGACCAGGGACCAAAAGTGGGACA GGAAAGCCAGCCTGGAGAGGTAGAGACTGAGCCTAGTCCCGGAGTGGAGGAGAAGGAACGGTTGACACAG AGGCTTGAACGTCTGCAGCAAGCGGTGGCAGAGCTGGAAGTTGACCGAAGCAAGCTGCAGTGCCACAATG CGCAGCTGCGGACCGCCTTGGAGCAGGTGGAACGAGAACGGAGGAAGCTGAAGAGGGATTCCGTGCGTGC ATCCCGGGCAGGATCACTGGAGGCCAGAGAAACTATGACTTCATCCCCCACACAGCAGGATGGGAGAGGC AGTCAGAGGGGTTCCTCGGACAGCGTGCTTGTGGTTGAGCTTCAGAGAGAGGTAGCCCTGCTGCGAGCCC AGCTGGCCTTGGAGCGAAAGCAGAGGCAGGACTACATTGCACGGTCCGTGCAGACCAGCCGTGAGCTAGC AGGCCTGCATCACAGCCTGTCTCACTCCCTCCTCACCGTGGCCCAGGCCCCTGAGGCCACTGTTCTGGAG GCTGAGACCCGAAAGCTGGATGAGTCTCTGAATCAGAGTCTGACATCCCCAGGGCCATGTTTGCTACATC CCAGTCTGGACACTACTCAAAATACCCACAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217223 representing NM_008383
Red=Cloning site Green=Tags(s) METGSPGLNMKPQSLQLVLEGQVLALQQQMAENQAASWRKLKNSQEAQKRQATLVRKLQAKVLQYRSWCQ DLEKRLEATGGLIPQRWESVEEPNLEQLLIRLEEEQQRCESLVEVNTELRLHMEKADVVNKALQEDVEKL TVDWSRARDELVRKESQWRMEQEFFKGYLRGEHGRLLNLWREVVTFRRHFLKMKSATDRDLTELKAEHAR LSGSLLTCCLRLTLRAQSRESSGSGRTEESEPARLLLLVAKTQALEKEAHEKSQELMQLKSHGDLEKAEL QDRVTELSALLTQSQKQNEDYEKMVKALRETMEILETNHAELMEHEASLSRNAQEEKLSLQQVIKAITQA LASVEEEDTVTQSSGHEDLLQSDCNGLSQFDPQDPDRALTLVQSVLTRRQQAVQDLRQQLSGCQEAMSFL QQQRDQWEEEGRALRERLQKLTGERDALAGQTVDLQGEVDSLSRERELLQKARGELQQQLEVLEQEAWRL RRMNMELQLQGDSAQGERLEQQEELHLAVRERERLQETLVGLEAKQSESLSELLTLREALESSRLEGELL KQERVEVAAALARAEQSIVELSGSENSLKAEVADLRAAAVKLGALNEALALDKVGLNQQLLQLEQENQSL CSRVEAAEQLRSALRVDLAEAERRREALWEKKTQLETQLQKAEEAGAELQAELRGTREEKEELKDKLSEA HHQQETATAHLEQLHQDAERQEETLARAVQEKEALVRERAALEVRLQAVERDRQDLTEHVLGLRSAKEQL ESNLFEAQQQNSVIQVTKGQLEVQIQTIIQAKEVIQGEVKCLKLELDAERTRAEQERDAVARQLAQAEQE GQASLERQKVAHEEEVNRLQEKWEKERSWLQQELDKTLETLERERAELETKLREQQTEMEAIRTQREEER SQADSALYQMQLETEKERVSLLETLLRTQKELADASQQLERLRQDMKIQKLKEQETTGMLQAQLQETQQE LKEAAQQHRDDLAAFQKDKLDLQKQVEDLMSQLVAHDDSQRLVKEEIEEKVKVAQECSRIQKELEKENAS LALSLVEKEKRLLILQEAYSVRQQELSSLRQDIQEAQEGQRELGVQVELLRQEVKEKEADFVAREAQLLE ELEASRVAEQQLRASLWAQEAKATQLQLQLRSTESQLEALVAEQQPENQAQAQLASLCSVLQQALGSACE SRPELRGGGDSAPTLWGPDPDQNGASRLFKRGSLPTALSPEAVALALQKLHQDVWKARQARDDLRDQVQK LVQRLTDTEAQKSQVHSELQDLQRQLSQSQEEKSKWEGRQNSLESELRDLHETAASLQSRLRQAELQKME AQNDRELLQASKEKLSAQVEHLQACVAEAQAQAGAAPVLEEDLRTARSALKLKNEELESERERAQALQEQ GELKVAQGKALQENLALLAQTLSNREREVETLQAEVQELEKQREMQKAALELLSLDLKKRSREVDLQQEQ IQELEQCRSVLEHLPMAVQEREQKLSVQRDQIRELENDREAQRSVLEHQLLDLEQKAQVIESQRGQIQDL KKQLGTLECLALELEESHHKVESQQKMITELEGQREIQRVALTHLTLDLEERSQELQAQSSQLHELENHS THLAKELQERDQEVTSQRQQIDELQKQQEQLAQALERKGQELVLQKERIQVLEDQRTLQTKILEEDLEQI KHSLRERSQELASQRQLVHERADDGKSPSKGQRGSLEHLKLILRDKEKEVECQQERIQELQGHMGQLEQQ LQGLHRKVGETSLLLTHREQETATLQQHLQEAKEQGELREQVLQGQLEEAQRDLAQRDHELETLRQEKQQ TQDQEESMKLKTSALQAALEQAHATLKERQGELEEHREQVRRLQEELEVEGRQVRALEEVLGDLRAESRE HEKAVLALQQRCAEQAQEHEAEARTLQDSWLQAQATLTEQEQELAALRAENQYSRRQEEAAVSQAEALQE ALSKAQAALQEKEQSLLEQAELSHTLEASTAALQATLDTCQARARQLEEALRIREGEIQAQALQHHEVTQ HLQQELCQKEEELRQLLEKAGARRSQENGIQEKQSLEQERQEETRRLLESLKELQLTVAQREEEILMLRE ASSPSHRALPAEKPALQPLPAQQELERLQTALRQTEAREIEWREKAQDLALSLAQSKASISSLQEITMFL QASVLERESEQQRLQEELVLSRQALEEQQSGGPHSTSRADQGPKVGQESQPGEVETEPSPGVEEKERLTQ RLERLQQAVAELEVDRSKLQCHNAQLRTALEQVERERRKLKRDSVRASRAGSLEARETMTSSPTQQDGRG SQRGSSDSVLVVELQREVALLRAQLALERKQRQDYIARSVQTSRELAGLHHSLSHSLLTVAQAPEATVLE AETRKLDESLNQSLTSPGPCLLHPSLDTTQNTHR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008383 |
| ORF Size | 7242 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008383.3, NP_032409.3 |
| RefSeq Size | 7919 bp |
| RefSeq ORF | 7245 bp |
| Locus ID | 16328 |
| UniProt ID | Q60952 |
| Cytogenetics | 2 H1 |
| Gene Summary | May be involved in ciliogenesis. Probably plays an important role in centrosome cohesion during interphase.[UniProtKB/Swiss-Prot Function] |
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