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Rpusd2 (NM_173450) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217107
Rpusd2 (tGFP-tagged) - Mouse RNA pseudouridylate synthase domain containing 2 (Rpusd2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $716.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rpusd2
Synonyms 4921503C21Rik; 9630001E10; BB231107
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217107 representing NM_173450
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCGGGGTGTCCCGGGCTGCCTACGGGACATTGTGCAGTGGCAGGTGGCGCTCTGGTCCCACAGCT
TTGTCCGGACTTGGGGTAGCTGTGGGAAGGCGATGACTGAGGCACTGTCTGCGCAAGCCGAGGCAGCGGG
CGGGCTGAAGGCTTTGGTTCAGCCAAATGGAGATGCTGGTAGCAACACGAGCGGAGAGCCGCTCCTGGAG
CGCCTGGAGCCGGCGGCGGTAGGGAAGCAGGTCCCGGAGAGCGGGGACCAGGCCCAGGGAGGCGAAGGGC
AGCTCCCGAGCAACGGAGAGCAGACCCCAGCTCCAGTCGCCGACTCAGGCAAGAGGAAAAAGCGGAGGGG
CGCCACTGGGGAACGGGTCGTGCCGCCTCCGAAGAAACGGCGCACCGGGGTGAGCTTTAGCGATGAGCAC
TTCGCAGAGACTACCTACTACTTCGAGGGCGGCCTGCGCAAGGTGCGGCCCTATTACTTTGACTTCCAGA
CCTACTGTAAAGGTCGCTGGGTGGGCCGCAGCTTGTTGCACGTCTTCAGCACCGAGTTCCGATCCCAGCC
ACTGTCTTACTACGAGGCCGCTGTCCGGGCAGGACGCCTGCATCTCAACGAGGAACCAGTCCAGGACCTC
AGCATCGTGCTCAAGGACAATGATTTCTTGCGGAACACAGTGCACAGACATGAGCCACCAGTCACAGCAG
AGCCTATCCGCCTGCTAGCTGAGAACAACGATGTGGTTGTTATAGACAAGCCGTCCTCCATTCCTGTGCA
CCCCTGTGGCCGCTTCCGACACAACACGGTCATCTTCATCCTAGGCAAGGAGCACCAACTGAAAGAGCTA
CACCCACTGCATCGGCTGGACCGCCTCACCTCAGGAGTGCTCATGTTTGCGAAGACAGCTGCAGTCTCGG
AGAAGATCCATGAGCAGGTTCGGGATCGGCAGCTGGAGAAGGAGTATGTATGCCGGGTTGCAGGGGAGTT
CCCTGACAAAGAGGTAATTTGTAAGGAACCCATCTTAGTGGTGTCTTACAAGGTAGGAGTGTGCCGTGTG
GATCCCCGGGGCAAGCCTTGTGAGACTATGTTCCAGAGGCTGAGCTACAATGGCCGCTCCAGTGTGGTAC
AGTGCCGACCACTTACAGGTCGCACTCACCAGATCCGAGTCCACCTCCAATTCCTGGGCCACCCGATTCT
CAATGACCCCATCTACAATTCAACTGCCTGGGGTCCTTCTCGAGGCCAGGGTGGCCACATTCCAAAGACA
GACGAGGAACTGCTCCGAGACCTGGTTGCAGAACATCAGGCCAAAGAAAGCCTGAGTATGCTAGATCTCT
GTGAGAGTGACCTGACGCCAGGGCTCATAGACTCGACAGCTCCCTCTTCAGAGTTGGCTAAAGACAGCCT
GGAAGGGTTGGCTACAGCAGCCCAGAAGATAGATGGAATAGCTGAGGCAGCCCCTCAGCATCTGGACACA
CCAGAGAAGGCAGCTAAAGCAGATGTGACTCAAGAAACAGACCCGCTCTGTGCCGAGTGCCGAGTGTTGC
GACAAGATCCCTTGCCCCAGGATCTCGTAATGTTCCTGCATGCCCTCCGCTATAAAGGGCCAGACTTTGA
GTACGTCTCACCCATCCCAGCCTGGGCACGGGATGACTGGCAAGAAGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217107 representing NM_173450
Red=Cloning site Green=Tags(s)

MWRGVPGCLRDIVQWQVALWSHSFVRTWGSCGKAMTEALSAQAEAAGGLKALVQPNGDAGSNTSGEPLLE
RLEPAAVGKQVPESGDQAQGGEGQLPSNGEQTPAPVADSGKRKKRRGATGERVVPPPKKRRTGVSFSDEH
FAETTYYFEGGLRKVRPYYFDFQTYCKGRWVGRSLLHVFSTEFRSQPLSYYEAAVRAGRLHLNEEPVQDL
SIVLKDNDFLRNTVHRHEPPVTAEPIRLLAENNDVVVIDKPSSIPVHPCGRFRHNTVIFILGKEHQLKEL
HPLHRLDRLTSGVLMFAKTAAVSEKIHEQVRDRQLEKEYVCRVAGEFPDKEVICKEPILVVSYKVGVCRV
DPRGKPCETMFQRLSYNGRSSVVQCRPLTGRTHQIRVHLQFLGHPILNDPIYNSTAWGPSRGQGGHIPKT
DEELLRDLVAEHQAKESLSMLDLCESDLTPGLIDSTAPSSELAKDSLEGLATAAQKIDGIAEAAPQHLDT
PEKAAKADVTQETDPLCAECRVLRQDPLPQDLVMFLHALRYKGPDFEYVSPIPAWARDDWQED

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173450
ORF Size 1659 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173450.3, NP_775626.1
RefSeq Size 5135 bp
RefSeq ORF 1662 bp
Locus ID 271842
UniProt ID Q149F1
Cytogenetics 2 E5
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Documents
Resources
"NM_173450" in other vectors (4)
SKU Description Size Price
MC218902 Rpusd2 (untagged) - Mouse RNA pseudouridylate synthase domain containing 2 (Rpusd2), (10ug) 10 ug
$567.00
MR217107 Rpusd2 (Myc-DDK-tagged) - Mouse RNA pseudouridylate synthase domain containing 2 (Rpusd2) 10 ug
$289.00 $516.00
MR217107L3 Lenti ORF clone of Rpusd2 (Myc-DDK-tagged) - Mouse RNA pseudouridylate synthase domain containing 2 (Rpusd2) 10 ug
$816.00
MR217107L4 Lenti ORF clone of Rpusd2 (mGFP-tagged) - Mouse RNA pseudouridylate synthase domain containing 2 (Rpusd2) 10 ug
$816.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.