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Galnt5 (NM_172855) Mouse Tagged ORF Clone

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SKU
MG217090
Galnt5 (tGFP-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 5 (Galnt5), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,051.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Galnt5
Synonyms 4832424J23
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217090 representing NM_172855
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAAGATCCGAAAGTTTTTCCGAGGAAGTGGGCGAGTCTTGGCATTTATATTTGCAGCGTCTGTCA
TCTGGTTGCTCTTTGACATGGCAGCTCTCCGCCTCTCATTCAGTGAGATCAACGCAGGGCTACTCAAAGA
AGATATCATAAGGAGGGAACATACGGGATTCAGAGTTGAGCCAGACCAAGTGAAGGTCCTTTACACCAGC
ATCAGAGGGATGGGGCTGCCTCAGAATGGAGCGTGGGGTAAGGAGAACTTTAGAAAAGCTGAGAACCATG
AACTCAAGGTTGAGGAGAATATGGACCAAGTCCAGAGGAAAGGGAAAATGCAGAACCTCCTGGGAAGGAG
AAAGGCTGTGCCTTTGTGGCATCTTGCACATCTGCAAACCCTCCCAGTGACAATTCCCATGCAGAAGACC
CAGGGGAGAGATAGCAAGCCTGAAGTCTCCTCCCAATACATGATGTCCAAGTGGATGACAGTGTTAGAGT
CTGAGAAGACCCCATTCACAGCTTCAAGAGGAGTTCCATTGACCAAAATAGCAGGACGTACAGAAACCTT
CGATAAAAAACAAGAGGCCCCCCAAAATTATAATGTCAGCAGCGATACATCAAAGCAAGCATCTGAAAGG
ACCCTGAATATGACCATCAGTGTCAAGACTGATAGATCAAAGCAAAAATCACAGACAGTAACAAAATTGA
GGATGCACTTTGCCAGCCCACCAATCCTGAAATCTGAGGAAGTCACAGTCATAAAGAAAGCTGAGGCTCA
GAGCAAAGACCTAAAACATGAAGCCCTTAAAGCGCTACCACTTCTTAAGTTCATTGCAGATATGGGTCAT
TTAAAGAACCAATCTACAAATGAGACACAGTTGGGAAGATTGCCAGAAGATGATGCAGCCAAAGTGGCGC
CAGGGAAGAAACTTAATTTCTCTGAAAGTCATGTTGTGATTATAACTAAAGAGGAAGAACTGAAGACAGA
CACCAAAGAGGTACCTAATTCTAAAACCCAAACTGTGTTTCCTAAATTACTGGGTGGAAGTCCACATAAA
CAAATCCCCAGAAATCAAAGCAAGACGTCATCCTCTCCCCCAGCTCTGAAGAAAGCAGTGTCTCAGTCCA
AGCCCACCATATCTGGGGGACTCCATACAGCAAGAAGCAACTTGACTGCCAAGGCTCCAACTGTGGGATA
CCAGCAAAGCCATGCAAATATCCCTGAAAACCCTGGAATGCACCACGTGTTCAGAATTGATGTGACCCTT
TCTCCAAGGGATCTCAATGCTCCGGGTCAGTTTGGGCGCCCTGTGGTTGTTCCCCCTGAGAAGAAGAAGG
AGGCCGAGCAGAGATGGAAAGAAGGAAACTTCAATGTCTACCTTAGTGATTTGATTCCAGTGGACAGAGC
CATTGAAGACACAAGGCCTGCTGGGTGTGCAGAGCAACTAGTTCACAATGACCTCCCAACCACCAGCATC
ATCATGTGCTTTGTAGATGAGGTGTGGTCCGCTCTACTGAGGTCTGTGCACAGCGTCCTCAACCGCTCCC
CTCCACACCTCATCAAGGAGATTCTGCTGGTGGATGACTTCAGCACCAAAGAATACCTCAAAGCTGATTT
GGATAAATACATGTCTCAATTTCCAAAAGTTCGGATTCTTCGCCTGAAGGAGAGACATGGCTTGATCAGA
GCGAGGCTGGCTGGGGCACAGAACGCAACAGGTGATGTGCTGACATTCCTAGACTCTCATGTGGAGTGTA
ATGTGGGCTGGCTGGAACCTCTACTGGAAAGAGTTTATTTAAATAGAAAGAAAGTGGCCTGTCCAGTAAT
TGAAGTCATAAATGACAAGGACATGAGTTACATGACAGTGGACAACTTCCAAAGAGGTGTCTTCACCTGG
CCCATGAACTTTGGCTGGAAAACAATCCCTCCAGATGTTGTTGCAAAAAATGGAATTAAAGAAACAGATA
TAATAAGGTGCCCAGTCATGGCAGGTGGATTATTTTCCATTGACAAAAGTTACTTTTATGAACTTGGAAC
ATATGACCCTGGTCTTGATGTTTGGGGAGGAGAAAATATGGAGCTATCATTCAAGGTCTGGATGTGTGGT
GGTGAAATTGAGATCATCCCCTGTTCCAGAGTGGGCCACATATTCAGAAACGACAATCCATATTCCTTCC
CCAAAGACCGTATGAAGACAGTGGAACGGAACCTGGTGCGGGTTGCTGAGGTTTGGCTTGATGACTACAG
GGAACTCTTCTATGGCCATGGGGATCACCTCATAGACCAAGGGCTGGATGTGGGCAACCTTACCCAGCAA
AGGGAGTTGCGGAAGAAGCTGAAATGCAAAAGTTTCAAGTGGTACTTGGACAATGTCTTTCCTGACTTGA
AGGCGCCCGTTGTGAGAGCTAGTGGTGTGCTTATTAATATGGCCTTGGGCAAGTGCGTTTCCATTGAAAA
TATCACAGTCACCCTCGAGGACTGCGATGGGAGCAGCCAGCTTCAACAATTTAATTATACCTGGGTAAGA
CTTATTAAGCACGGAGAGTGGTGTGTGGCACCCATCCCTGAAAAAGGATCCCTGACACTGTACCATTGTG
ACAACAGAAACAACAGGCTTAAATGGCTGCACAAATCAGCATCTGCCTTCCATCCAGAACTGGTGGATCA
CATTGTTTTTGAAAACTATCAGCAGTTATTATGTATGGAAGGGAATTTCTCTCAGAAGACCCTGAAACTG
GCTGCTTGTAACCCAATGGAACTGCAACAAAAGTGGAAATTTGAAAAATACTACGAAGTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217090 representing NM_172855
Red=Cloning site Green=Tags(s)

MNKIRKFFRGSGRVLAFIFAASVIWLLFDMAALRLSFSEINAGLLKEDIIRREHTGFRVEPDQVKVLYTS
IRGMGLPQNGAWGKENFRKAENHELKVEENMDQVQRKGKMQNLLGRRKAVPLWHLAHLQTLPVTIPMQKT
QGRDSKPEVSSQYMMSKWMTVLESEKTPFTASRGVPLTKIAGRTETFDKKQEAPQNYNVSSDTSKQASER
TLNMTISVKTDRSKQKSQTVTKLRMHFASPPILKSEEVTVIKKAEAQSKDLKHEALKALPLLKFIADMGH
LKNQSTNETQLGRLPEDDAAKVAPGKKLNFSESHVVIITKEEELKTDTKEVPNSKTQTVFPKLLGGSPHK
QIPRNQSKTSSSPPALKKAVSQSKPTISGGLHTARSNLTAKAPTVGYQQSHANIPENPGMHHVFRIDVTL
SPRDLNAPGQFGRPVVVPPEKKKEAEQRWKEGNFNVYLSDLIPVDRAIEDTRPAGCAEQLVHNDLPTTSI
IMCFVDEVWSALLRSVHSVLNRSPPHLIKEILLVDDFSTKEYLKADLDKYMSQFPKVRILRLKERHGLIR
ARLAGAQNATGDVLTFLDSHVECNVGWLEPLLERVYLNRKKVACPVIEVINDKDMSYMTVDNFQRGVFTW
PMNFGWKTIPPDVVAKNGIKETDIIRCPVMAGGLFSIDKSYFYELGTYDPGLDVWGGENMELSFKVWMCG
GEIEIIPCSRVGHIFRNDNPYSFPKDRMKTVERNLVRVAEVWLDDYRELFYGHGDHLIDQGLDVGNLTQQ
RELRKKLKCKSFKWYLDNVFPDLKAPVVRASGVLINMALGKCVSIENITVTLEDCDGSSQLQQFNYTWVR
LIKHGEWCVAPIPEKGSLTLYHCDNRNNRLKWLHKSASAFHPELVDHIVFENYQQLLCMEGNFSQKTLKL
AACNPMELQQKWKFEKYYEV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172855
ORF Size 2790 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172855.3, NP_766443.2
RefSeq Size 3247 bp
RefSeq ORF 2793 bp
Locus ID 241391
UniProt ID Q8C102
Cytogenetics 2 C1.1
Summary Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward EA2 peptide substrate, but has a weak activity toward Muc2 or Muc1b substrates (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172855" in other vectors (4)
SKU Description Size Price
MC222722 Galnt5 (untagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 5 (Galnt5), (10ug) 10 ug
$938.00
MR217090 Galnt5 (Myc-DDK-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 5 (Galnt5) 10 ug
$589.00 $851.00
MR217090L3 Lenti ORF clone of Galnt5 (Myc-DDK-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 5 (Galnt5) 10 ug
$1,151.00
MR217090L4 Lenti ORF clone of Galnt5 (mGFP-tagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 5 (Galnt5) 10 ug
$1,151.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.