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Arhgef17 (NM_001081116) Mouse Tagged ORF Clone

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SKU
MG217055
Arhgef17 (tGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 17 (Arhgef17), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $2,067.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Arhgef17 or NM_001081116"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arhgef17
Synonyms 8030463K16; AI428794; AW558066; BC035332; mKIAA0337
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217055 representing NM_001081116, codon optimized.
Due to the complexity of NM_001081116, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACGGTAGCCCAAGACCCCCCTTGTACCGCTCTGTTTCTTTCAAACTTCTGGAACGGTGGAGCG
GCGGCCCTGGCCCCAGAGAGGAAGACGCAGATACACCAGGCCTCAGGAGAAGGGCTTCCTGCCGACCAGC
GGCTGCCGTGCCTGGACAACCGTCAAGACGGGTCAGCAAGCTGGCCTCTGGACCTCCTGCGGCTCCCGCC
CAGCCTAGGCCCCTGCGCTCTCTGTCTCCAAGCGTGAGACAGTTGTCTAGGCGCTTTGACGCAGCTGGGT
TGGACGACGATTCCACTGGCACCCGCGACGGTGGATGCTCTAGTGGTACCACTGAAGAGGCAGCTGAAGG
CAGTGAAAGGGGAGCTTGGCCAAGCGTTACCGAAATGCGCAAGCTCTTCGGAGGGCCCTCCAGCCGACGC
CCTAGCATGGACAGCGAAGCACTCGGCAGCACCAGCCCTGACCGAGTGTCTTGGGAGCCTCCAACTCGGG
ATCCAAGGCAGCCTCCCACGCCCCCACCCCGCACTTGCTTCCCTCTGGCCGGTTTGAGGAGTGCGCGGCC
GCTGTCCGGGCCCGGAATTGAAGGCAGGAGACGGCGGCAGCACCAACAACAGGAGCGAGCCCAGAGGCCC
GCCGACGGTCTTCATAGCTGGCACAGCTTTTCCCAACCCCAGGCAGGCGCAAGGGCTAGCTCCTCTTCAA
GCATCGCCAGTAGCTACCCTGTGTCACGAAGTCGCGCCGCGTCTTCCAGCGAGGAAGAGGAGGAAGGGCC
TCAGTCACAATTGGGCCCACAGAGCCCGGCCTACCTGGGAGGTCATAGTTCCGGGAGTGACGAAGACCCA
AATGGAGAGGACGGACGCCGCTGGAGAGGCAGAGGACTCAGACCCGGTCGATCTCAGCTCGTGCATGGCT
GTTCCCAGGACTCCGATGAACTGAACCCAGGCGGTCTGGGTTCTGCCGGTGGTGTCGGCTCCCCTGAGCC
CCCTACCAGCCCCAGGACATCAATGGACGAGTGGGGAACTGGGACACAGCCCTGCCTGCCTGGACCCCAG
GAGTCCCTTAGACCTATGTCCGATACGGGGGGCGCTCCTTTTAGAGTCGCAAAAGTCTCTTTTCCCGCTT
ATCTGGCCAGTCCCGCAGGGAGCCGGGGAAGTAGTAGGTATAGTAGCACCGAGACACTGAAGGATGATGA
TTTGTGGTCCTCAAGGTCTTCCGTAGGGTGGGGCGTGTATAGAAGTCCCTCTTTTGGTACTGGCGACGGA
CTGCTCCTCCGGCCCCAAACACGCAGTAGGAGCAAAGGACCCGTGGGAACCGCTCGGCCTCTGCGCGACG
GCGGGCTGGACCTGGACAAGAATCGCCAGCGAAAAAGCCTCAGCAATCCAGACATCGCTAGCGAGACTTT
GACATTGCTGTCCTTTCTTCGAAGCGACCTGTCCGAACTTCGGGTGCGAAAGCCCAGTGGTGGGCCAGGG
AACCGACCCCTCGACGGGAGGGACTCACCATCTGCCGGCTCCCCCATGGAACAATCCGAGTCCACTCTCT
CCCAGAGCCCCACTTCTCCTACCACCCGGCCAACTCTGAAAGACCTGACGGCTACACTGAGACGAGCCAA
AAGCTTTAGCTGCAGCGAAAAACCGATGGCTCGCCGGCTGCTGCGCACTTCTGCTCTGAAACCTAGCTCA
AGCGAGCTGCTGCTTGCTGGAAGCGGAGCCGAGGAAGACCCGCTGCCACTTGTGGTTCAGGATCAATATG
TCCAGGAGGCCAGACAGGTCTTCGAGAAAATCCAAAGGATGGGGGCCCAACAGGACGACGGAAATGACGT
GTGTCCGACCAGCCCCGATTGGGCTGGCGATATGACTCAAGGCCACAGATCCCAGGAAGAGCTTAGCGGC
CCCGAGTCAAATCTGACGGATGAGGGAATCGGCGCCGATCCGGAACCTCTGGGCGCTGCTTTCTGCTCAC
TGGACCCAGCTGGGGTCTGGCGACCTTTGTCTTCCACATCAGCTCAAACCAATCACCACCTCGGTGCTGG
AACCGAGGACAGTCTCGGGGGAAGAGCTCTTGTTTCCCCCGAAACACCACCTACTCCTGGTGCACTGAGA
AGACGGCGAAAAGTACCCCCGAGTGGACCCAACGGGACAGAGTTGTCCAATGGAGAGGCATCCGAGGCCT
ACCGCAGCCTGAGCGACCCCATTCCTCAACGGCACCGCGCTGCAACGTCAGAGGAGCCGTCCGGCTTTAG
CGTAGACTCTAACCTGCTGGGGTCTTTGAACAGCAAAACTGGCCTGCCCGTTACGCCAGCGATGGACGAG
GGACTCACGTCAGGGCATTCAGACTGGTCTGTGGTGAGCGAAGAAAACAAGGATTACCAAGAGGTGATCC
AGTCCATTGTCCAGGGTCCGGGAGCACTCGGGAGGATGGGGGAGGATCGAATTGCTGGGAAGACACCAAA
AAAGAAATCATTGAGCGACCCCAGCCGCCGCGGAGAATTGACAGGGCCAGGGTTCGAGGGTCCAGGCGGC
GAACCAATTCGAGAAGTTGAGCCTATGCTTCCTCCTTCTTCCTCTGAGCCAATCCTTGCCGAGCAGTGGA
CTGAGCCCGAAGATCCAGCTCCCGCCCGGGGCCGGGCACAATCAGAGCGGTCCCTGCCAGCCCCCCCAGC
CTCCAGCACTGCTCACCATGACTTTCACCTGGACCCTAAATTGACCTCAGTGCTGAGCCCCAGACTGACA
CGGCGGGGGTCAAAGAAGAGGCCTGCACGGTCTTCCCACCAGGAGCTGCGCCGGGAGGAGGGGAACCAGG
ATCAAACCGGATCATTGACTCAGACTAGGTCTTCCAGTAAGCACGTGCGCCACGCTTCCGTCCCTGCCAC
ATTTACTCCTATCGTGGTGCCCGAGCCAGCCATGTCCGTGGGCCCCCCCGTCGCCGCTCCGGAGCCAGTT
GGCTTCCCAGTGAGGGGCCACCCCGCTCTCCAGGCACCTTCTCTGGAGGATGTGACCAAGCGGTACATGT
TGACTCTGCACTCCGGGGATGTACCCGCCCCCGGCCCTGTCGATCTTCCATGTCTGCCTCCTTCCGCGCC
TCCCAGCACCGAGACAAAGCCGAGCGGCGCTGCCAGGGCCACACCAGACGAGCCAGCTCCTGCAAGCAAG
TGTTGCAGCAAACCTCAGGTTGATATGCGGAAGCACGTCACCATGACATTGCTGGACACAGAGCAGAGCT
ATGTGGAATCCCTCAGGACCCTGATGCAAGGCTATATGCAACCTCTGAAACAACCCGAGAACTCCCTCCT
CTGTGACCCATCCTTGGTAGACGAGATTTTTGACCAAATCCCTGAGCTGTTGGAACACCACGAGCAGTTT
TTGGAACAGGTCCGCCATTGTGTGCAGACATGGCACGCACAGCAAAAGGTGGGTGCCCTCCTGGTCCAGA
GTTTTTCTAAGGACGTGCTGGTCAATATTTATTCTGCCTATATCGACAACTTTCTGAATGCAAAGGACGC
AGTTCGGGTGGCAAAAGAAGCCAGACCCGCTTTTCTGAAATTCCTGGAACAAAGCATGCGCGAGAACAAG
GAGAAGCAAGCCCTGTCCGACCTCATGATTAAACCGGTGCAGAGAATCCCTCGATACGAGCTGCTGGTCA
AAGATCTGCTGAAACACACACCCGAGGACCACCCAGATCATCCACTGCTGCTGGACGCTCAAAGGAACAT
CAAACAAGTCGCTGAACGGATCAACAAGGGCGTACGGAGCGCCGAGGAAGCCGAAAGGCACGCCAGGGTC
CTGCAGGAGATCGAGGCTCACATCGAGGGCATGGAAGACCTTCAGGCACCGCTGCGCCGCTTTCTTAGGC
AGGAAATGGTAATCGAAGTTAAAGCAATTGGAGGAAAAAAAGATAGATCCTTGTTTCTCTTTACGGACCT
TATTGTCTGCACGACGCTCAAACGCAAGAGCGGAAGCCTTAGAAGGTCTTCTATGAGTCTTTATACCGCC
GCATCCGTGATCGATACTGCCTCCAAGTACAAGATGCTTTGGAAGCTGCCCTTGGAAGACACCGACATTA
TAAAGGGAGCATCTCAGGCCACAAACCGGGAGACAATCCAGAAGGCCATCTCACGCCTGGATGAGGACCT
CGCAACACTGGGCCAGATGTCAAAGCTCTCCGAGAGCCTCGGGTTCCCTCACCAGAGTCTGGATGATGCT
CTCCGCGACCTCTCTGCCGCCATGCACAGGGACCTCTCCGAGAAGCAAGCACTGTGTTGCTCACTCGCAT
TCCCTCCTACAAAGCTTGAGTTGTGTGCCACACGGCCCGAGGGCACAGATTCATATATCTTCGAATTTCC
TCACCCAGACGCCAGATTGGGCTTTGAGCAAGCTTTTGACGAGGCTAAGCGCAAACTCGCCAGCAGCAAG
AGCTGCCTCGATCCCGAATTCCTGAAGGCTATCCCGATCATGAAGACTAGATCCGGAATGCAGTTCTCAT
GTGCCGCCCCTACTTTTAGCTCTTGTCCAGAACCGGCCCCTGAGGTGTGGGTATGTAACTCCGATGGGTA
CGTAGGGCAGGTGTGCCTGCTCAGTCTCAGAGCTGAGCCAGACGTGGAGGCTTGTATTGCCGTCTGTTCA
GCTCGAATTCTCTGCATTGGAGCCGTCCCTGGCCTGCAGCCTCGCTGTCCCCGGGAGCAGCCCGAGCCTC
TCAGAAATCCCCCCGAAACAACCCTCGAATCTACAGGACCTGAACTGGACGTCGAGGCCACCGCCGAGGA
GGAAGCGGCCACCACCCTCGCAGAACCTGGACCTCAACCTTGCCTTCACATTAGTATCTCCGGGTCAGGC
CTGGAGATGGAACCCGGCCCTGCTAAGGGCGATCCTCAGCCAGAGCTTGTCCCCTTCGATAGTGATTCTG
ATGACGAATCCTCCCCCTCCCCAAGTGGTACGTTGCAGTCCCAGGCCTCCCAATCTACAATCTCTAGCTC
ATTTGGTAATGAGGAAACACCGAGCTCCAAGGAGGCAACCGCAGAGACAACAAGCTCCGAGGAGGAGCAA
GAGCCCGGATTTCTGTCACTTAGCGGCAGTTTCGGCCCAGGAGGCCCTTGTGGAACATCTCCGATGGACG
GGAGGGCCCTTCGACGCTCTAGCAGGGGGTCATTTACCAGAGGATCTCTGGAAGATCTTCTGAGCGTCGA
TCCAGAGGCATACCAGAGCTCAGTGTGGCTGGGTACCGAGGATGGCTGCGTGCACGTTTACCAGTCCAGT
GATTCCATTCGGGACCGCAGGAACTCCATGAAGCTGCAGCATGCTGCTAGCGTGACCTGCATCCTGTATC
TCAATAACAAGGTCTTCGTATCTCTGGCAAACGGCGAACTGGTTGTCTACCAGCGGGAGGCTGGGCGATT
CTGGGATCCTCAGAATTTTAAGTCAATGACTCTCGGCTCACAGGGTAGCCCTATCACCAAAATGGTTTCC
GTGGGAGGACGACTGTGGTGCGGATGTCAAAACAGAGTGCTTGTCCTCTCCCCCGACACTCTTCAACTGG
AGCATACCTTTTATGTGGGTCAGGACAGTTCCCGGTCAGTAGCATGCATGGTTGACAGCAGTCTGGGAGT
GTGGGTCACACTGAAAGGCTCAGCTCACGTGTGCCTGTATCACCCCGACACTTTTGAGCAGCTCGCCGAG
GTTGATGTTACACCACCTGTGCACAGAATGCTTGCAGGCAGCGATGCTATCATCAGACAGCACAAAGCGG
CGTGCCTGAGGATCACAGCTTTGCTTGTGTGTGCCGAACTGCTCTGGGTTGGCACCTCAGCCGGCGTGGT
GCTCACAATTCCAACAAGCCCCTCAACCGTGTCCTGTCCAAGGGCCCCACTGAGTCCCGCCGGACTCTGC
CAGGGCCACACTGGTCACGTCAGATTTCTGGCCGCCGTGCAGCTGCCAGAAGGATTTAACCTGCTGTGCT
CTACTCCCCCACCGCCTCCGGATACCGGACCCGAGAAACTGCCATCTCTGGACCACCGAGACAGCCCTCG
GCGCAGAGGACCAACCAGCGCCCGGCCAAAAATGCTGGTTATCTCCGGCGGTGACGGCAGCGAAGACTTC
AGGCTCAGCTCAGGCGGAGGAGGTTCTTCCGAGACGGTGGGTAGAGATGACTCCACGAATCACCTTCTCT
TGTGGCGCGTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217055 representing NM_001081116
Red=Cloning site Green=Tags(s)

MADGSPRPPLYRSVSFKLLERWSGGPGPREEDADTPGLRRRASCRPAAAVPGQPSRRVSKLASGPPAAPA
QPRPLRSLSPSVRQLSRRFDAAGLDDDSTGTRDGGCSSGTTEEAAEGSERGAWPSVTEMRKLFGGPSSRR
PSMDSEALGSTSPDRVSWEPPTRDPRQPPTPPPRTCFPLAGLRSARPLSGPGIEGRRRRQHQQQERAQRP
ADGLHSWHSFSQPQAGARASSSSSIASSYPVSRSRAASSSEEEEEGPQSQLGPQSPAYLGGHSSGSDEDP
NGEDGRRWRGRGLRPGRSQLVHGCSQDSDELNPGGLGSAGGVGSPEPPTSPRTSMDEWGTGTQPCLPGPQ
ESLRPMSDTGGAPFRVAKVSFPAYLASPAGSRGSSRYSSTETLKDDDLWSSRSSVGWGVYRSPSFGTGDG
LLLRPQTRSRSKGPVGTARPLRDGGLDLDKNRQRKSLSNPDIASETLTLLSFLRSDLSELRVRKPSGGPG
NRPLDGRDSPSAGSPMEQSESTLSQSPTSPTTRPTLKDLTATLRRAKSFSCSEKPMARRLLRTSALKPSS
SELLLAGSGAEEDPLPLVVQDQYVQEARQVFEKIQRMGAQQDDGNDVCPTSPDWAGDMTQGHRSQEELSG
PESNLTDEGIGADPEPLGAAFCSLDPAGVWRPLSSTSAQTNHHLGAGTEDSLGGRALVSPETPPTPGALR
RRRKVPPSGPNGTELSNGEASEAYRSLSDPIPQRHRAATSEEPSGFSVDSNLLGSLNSKTGLPVTPAMDE
GLTSGHSDWSVVSEENKDYQEVIQSIVQGPGALGRMGEDRIAGKTPKKKSLSDPSRRGELTGPGFEGPGG
EPIREVEPMLPPSSSEPILAEQWTEPEDPAPARGRAQSERSLPAPPASSTAHHDFHLDPKLTSVLSPRLT
RRGSKKRPARSSHQELRREEGNQDQTGSLTQTRSSSKHVRHASVPATFTPIVVPEPAMSVGPPVAAPEPV
GFPVRGHPALQAPSLEDVTKRYMLTLHSGDVPAPGPVDLPCLPPSAPPSTETKPSGAARATPDEPAPASK
CCSKPQVDMRKHVTMTLLDTEQSYVESLRTLMQGYMQPLKQPENSLLCDPSLVDEIFDQIPELLEHHEQF
LEQVRHCVQTWHAQQKVGALLVQSFSKDVLVNIYSAYIDNFLNAKDAVRVAKEARPAFLKFLEQSMRENK
EKQALSDLMIKPVQRIPRYELLVKDLLKHTPEDHPDHPLLLDAQRNIKQVAERINKGVRSAEEAERHARV
LQEIEAHIEGMEDLQAPLRRFLRQEMVIEVKAIGGKKDRSLFLFTDLIVCTTLKRKSGSLRRSSMSLYTA
ASVIDTASKYKMLWKLPLEDTDIIKGASQATNRETIQKAISRLDEDLATLGQMSKLSESLGFPHQSLDDA
LRDLSAAMHRDLSEKQALCCSLAFPPTKLELCATRPEGTDSYIFEFPHPDARLGFEQAFDEAKRKLASSK
SCLDPEFLKAIPIMKTRSGMQFSCAAPTFSSCPEPAPEVWVCNSDGYVGQVCLLSLRAEPDVEACIAVCS
ARILCIGAVPGLQPRCPREQPEPLRNPPETTLESTGPELDVEATAEEEAATTLAEPGPQPCLHISISGSG
LEMEPGPAKGDPQPELVPFDSDSDDESSPSPSGTLQSQASQSTISSSFGNEETPSSKEATAETTSSEEEQ
EPGFLSLSGSFGPGGPCGTSPMDGRALRRSSRGSFTRGSLEDLLSVDPEAYQSSVWLGTEDGCVHVYQSS
DSIRDRRNSMKLQHAASVTCILYLNNKVFVSLANGELVVYQREAGRFWDPQNFKSMTLGSQGSPITKMVS
VGGRLWCGCQNRVLVLSPDTLQLEHTFYVGQDSSRSVACMVDSSLGVWVTLKGSAHVCLYHPDTFEQLAE
VDVTPPVHRMLAGSDAIIRQHKAACLRITALLVCAELLWVGTSAGVVLTIPTSPSTVSCPRAPLSPAGLC
QGHTGHVRFLAAVQLPEGFNLLCSTPPPPPDTGPEKLPSLDHRDSPRRRGPTSARPKMLVISGGDGSEDF
RLSSGGGGSSETVGRDDSTNHLLLWRV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081116
ORF Size 6171 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081116.1, NP_001074585.1
RefSeq Size 10250 bp
RefSeq ORF 6174 bp
Locus ID 207212
UniProt ID Q80U35
Cytogenetics 7 E2
Summary Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPases.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081116" in other vectors (2)
SKU Description Size Price
MC225155 Arhgef17 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 17 (Arhgef17), (10ug) 10 ug
$1,868.00
MR217055 Arhgef17 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 17 (Arhgef17). Note: ORF is codon optimized 10 ug
$1,867.00

file-alt Citations

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