Tcn2 (NM_001130458) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG217054
Tcn2 (tGFP-tagged) - Mouse transcobalamin 2 (Tcn2) transcript variant 2, (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tcn2
Synonyms AW208754; Tcn-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217054 representing NM_001130458
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCTCCTGAAGGCGCTGCTGCTCCTTTCTGGAGTCTTTGGGGCTCTTGCTGAATTCTGTGTAATAC
CACGGATAGACAGCCAGCTGGTAGAGAAGTTGGGTCAACGTCTTTTACCTTGGATGGATCGGCTTTCCTC
AGAGCAGTTGAACCCCAGCGTCTTCGTTGGCCTGCGACTTTCCAGCATGCAGGCTGGGACCAAAGAGGAC
CTCTACCTTCACAGCCTCAAGATCCATTACCAGCAGTGCCTCCTGCGGTCCACCTCCAGCGATGATAACA
GTAGTTGCCAGCCCAAACTTTCCGGGGGCAGCCTGGCGCTCTACCTGCTTGCTCTCAGGGCCAACTGCGA
ATTCTTCGGGAGCCGAAAGGGTGACAGGTTGATCTCACAGCTCAAGTGGTTCTTGGAGGATGAGAAGAAG
GCCATTGGGCACAATCATGAAGGCCACCCCAACACCAACTATTACCAGTATGGCCTCAGCATCCTGGCAC
TGTGTGTCCACCAGAAGCGGCTCCATGACAGCGTGGTGGGCAAGCTCCTGTATGCTGTTGAACATGACTA
CTTTACCTACCAGGGCCACGTCTCTGTGGACACAGAAGCCATGGCCGGCTTGGCCCTCACCTGTTTGGAG
CGATTCAATTTCAACTCTGATCTGAGACCACGAATCACCATGGCTATCGAGACAGTGCGAGAGAAAATCC
TGAAGTCCCAGGCCCCTGAGGGCTACTTTGGGAATATCTATAGCACCCCACTGGCACTACAGATGCTCAT
GACATCTCCTGCATCCGGGGTAGGGCTGGGCACAGCGTGCATCAAGGCAGGGACATCCCTGCTGCTCAGC
CTACAGGATGGGGCCTTCCAAAATCCTCTGATGATTTCCCAGCTGCTACCCATCCTGAACCACAAGACCT
ACTTGGATCTTATCTTCCCAGACTGCCAGGCATCCAGGGTCATGTTGGTGCCAGCTGTTGAGGACCCTGT
CCACATCTCAGAAGTCATCAGCGTCACGCTGAAGGTCGCCAGTGCCCTGTCACCATACGAACAGACATTT
TTTGTCTTTGCTGGATCTTCCTTGGAAGATGTCCTGAAGTTGGCACAAGATGGCGGAGGATTCACGTATG
GGACCCAGGCCTCCTTGTCAGGCCCCTACCTGACATCTGTACTAGGGAAAGATGCTGGGGACCGAGAATA
TTGGCAGCTCCTCCGAGCCCCTGACACACCCCTGCTGCAAGGCATTGCTGACTACAAACCCCAGGACGGG
GAAACCATTGAGCTGCGGCTAGTTAGATGG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217054 representing NM_001130458
Red=Cloning site Green=Tags(s)

MELLKALLLLSGVFGALAEFCVIPRIDSQLVEKLGQRLLPWMDRLSSEQLNPSVFVGLRLSSMQAGTKED
LYLHSLKIHYQQCLLRSTSSDDNSSCQPKLSGGSLALYLLALRANCEFFGSRKGDRLISQLKWFLEDEKK
AIGHNHEGHPNTNYYQYGLSILALCVHQKRLHDSVVGKLLYAVEHDYFTYQGHVSVDTEAMAGLALTCLE
RFNFNSDLRPRITMAIETVREKILKSQAPEGYFGNIYSTPLALQMLMTSPASGVGLGTACIKAGTSLLLS
LQDGAFQNPLMISQLLPILNHKTYLDLIFPDCQASRVMLVPAVEDPVHISEVISVTLKVASALSPYEQTF
FVFAGSSLEDVLKLAQDGGGFTYGTQASLSGPYLTSVLGKDAGDREYWQLLRAPDTPLLQGIADYKPQDG
ETIELRLVRW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001130458
ORF Size 1290 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001130458.1, NP_001123930.1
RefSeq Size 2058 bp
RefSeq ORF 1293 bp
Locus ID 21452
UniProt ID O88968
Cytogenetics 11 2.76 cM
Summary Primary vitamin B12-binding and transport protein. Delivers cobalamin to cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001130458" in other vectors (4)
SKU Description Size Price
MC209501 Tcn2 (untagged) - Mouse transcobalamin 2 (Tcn2), transcript variant 2, (10ug) 10 ug
$503.00
MR217054 Tcn2 (Myc-DDK-tagged) - Mouse transcobalamin 2 (Tcn2), transcript variant 2 10 ug
$289.00 $457.00
MR217054L3 Lenti ORF clone of Tcn2 (Myc-DDK-tagged) - Mouse transcobalamin 2 (Tcn2), transcript variant 2 10 ug
$757.00
MR217054L4 Lenti ORF clone of Tcn2 (mGFP-tagged) - Mouse transcobalamin 2 (Tcn2), transcript variant 2 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.