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Elovl7 (NM_029001) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217050
Elovl7 (tGFP-tagged) - Mouse ELOVL family member 7 elongation of long chain fatty acids (yeast) (Elovl7), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Elovl7 or NM_029001"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Elovl7
Synonyms 9130013K24Rik; AI840082
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217050 representing NM_029001
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTCAGTGATCTTACATCGAGGACTGTGCGTTTTTATGATAATTGGATCAAAGATGCTGATCCGA
GAGTTGAAGACTATCTCCTCATGTCCTCGCCTCTGCCACAAACCATCATCCTGGGCCTCTATGTCTATTT
TGTTACGTCTCTGGGACCAAAGCTCATGGAGAACCGGAAGCCCTTTGAACTCAAGAAAGCGATGATAACG
TACAATTTTTTCATAGTACTGTTTTCTGTGTATATGTGTTATGAGTTTGTGATGTCTGGCTGGGGTACAG
GTTACTCGTTTCGATGTGACATTGTTGACTATTCTCAGTCGCCAAGAGCAATGAGGATGGTGCACACCTG
CTGGCTTTATTACTTCTCCAAATTCATCGAGCTGTTAGACACTATCTTTTTTGTTCTGCGTAAGAAAAAC
AGCCAAGTGACTTTCCTTCATGTCTTCCATCATACGATCATGCCGTGGACCTGGTGGTTTGGAGTCAAAT
TTGCTGCAGGTGGTCTGGGAACATTCCATGCCTTTTTAAATACAGCTGTGCACGTGGTCATGTATTCTTA
CTATGGGCTATGTGCAATGGGACCAGCCTACCAGAAGTATTTATGGTGGAAAAAACATTTGACATCTTTG
CAGCTTGTCCAGTTTGTTCTTGTCACTATCCACATAGGCCAGATCTTCTTCATGGAGGACTGCAATTACC
AGTACCCAGTTTTTCTGTATATCATCATGAGTTACGGATGCATCTTCCTGCTGCTCTTTCTCCACTTTTG
GTACCGTGCTTACACCAAGGGTCAGAGGTTGCCCAAAACTCTAGAAAATGGAAATTGCAAAAGCAAGCGC
CAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217050 representing NM_029001
Red=Cloning site Green=Tags(s)

MAFSDLTSRTVRFYDNWIKDADPRVEDYLLMSSPLPQTIILGLYVYFVTSLGPKLMENRKPFELKKAMIT
YNFFIVLFSVYMCYEFVMSGWGTGYSFRCDIVDYSQSPRAMRMVHTCWLYYFSKFIELLDTIFFVLRKKN
SQVTFLHVFHHTIMPWTWWFGVKFAAGGLGTFHAFLNTAVHVVMYSYYGLCAMGPAYQKYLWWKKHLTSL
QLVQFVLVTIHIGQIFFMEDCNYQYPVFLYIIMSYGCIFLLLFLHFWYRAYTKGQRLPKTLENGNCKSKR
H

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029001
ORF Size 843 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029001.5, NP_083277.3
RefSeq Size 5453 bp
RefSeq ORF 846 bp
Locus ID 74559
UniProt ID Q9D2Y9
Cytogenetics 13 D2.1
Summary Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme with higher activity toward C18 acyl-CoAs, especially C18:3(n-3) acyl-CoAs and C18:3(n-6)-CoAs. Also active toward C20:4-, C18:0-, C18:1-, C18:2- and C16:0-CoAs, and weakly toward C20:0-CoA. Little or no activity toward C22:0-, C24:0-, or C26:0-CoAs. May participate in the production of saturated and polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029001" in other vectors (4)
SKU Description Size Price
MC211536 Elovl7 (untagged) - Mouse ELOVL family member 7, elongation of long chain fatty acids (yeast) (Elovl7), (10ug) 10 ug
$330.00
MR217050 Elovl7 (Myc-DDK-tagged) - Mouse ELOVL family member 7, elongation of long chain fatty acids (yeast) (Elovl7) 10 ug
$289.00 $300.00
MR217050L3 Lenti ORF clone of Elovl7 (Myc-DDK-tagged) - Mouse ELOVL family member 7, elongation of long chain fatty acids (yeast) (Elovl7) 10 ug
$600.00
MR217050L4 Lenti ORF clone of Elovl7 (mGFP-tagged) - Mouse ELOVL family member 7, elongation of long chain fatty acids (yeast) (Elovl7) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.