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Aen (NM_001162939) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217018
Aen (tGFP-tagged) - Mouse apoptosis enhancing nuclease (Aen) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
  $703.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Aen
Synonyms 2700083B06Rik; Isg20l1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217018 representing NM_001162939
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGCAGGGGAAGTCCCCGAGTCTACTCAGTGTCCATCTCTCACCAGCCTGAATACCAAGGATGTGG
TTCGGAGAAGACACAAGAGGAGAAGCCGGCAGCACCAGCGATTCATGGCCCGCAAGGCCTTGCTTCAGGA
GCAGGAGCTGTTGAGCACGGCACCAGGGCCAGGTCTCTGCCTTCTGCCTTCACCATCACAGATGCCAGCG
GTCACAGAGGCTTCGGACAGCAGGAGGCAACGTCCCAAGGCCAGGTCCGGCAGCAATGGCCTGTGCAGCA
AAAAGTCTGTCCCCAGAGAAGCTCCAAGACCTGGGCCCATCAAGTGTGTGGCCATTGACTGTGAGATGGT
GGGTACGGGCCCCCAAGGACGGGTGAGCGAGCTAGCCCGCTGCTCTGTGGTGAGCTACAGTGGTGACGTT
CTCTATGACAAATACATCCGGCCTGAAATGCCCATTGTGGACTATCGCACCCGCTGGAGTGGCATCACCC
GCCAGCACATGCACAAGGCTATCCCCTTTCAGGTGGCCCAGAAGGAGATCCTTAAGCTGTTGAAGGGCAA
GGTGGTGGTGGGGCACGCACTGCACAATGACTTCCAGGCCCTGAAGTACGTCCACCCTCGGAGCCAGACC
CGGGATACCACTTATGTCCCAAACCTGCTCAGCCAGCCCAGCTCCCTTATCCGAACTCGGGTCTCTCTTA
AGGACCTGGCCTTGAACCTGCTGCACAAGAAGATCCAGGTCGGTCACCAGGGCCACTCATCTGTGGAAGA
TGCCATGACAGCCATGGAGTTGTACCAGCTGGTGGAGGTGCAGTGGGAGCAGCAAGTGGCCAGCTCTGCC
CAGGCCCCTGCTGAGGACAGGGGGCCCGACAGCAGCACTGACGTGGAGCAGTACATGGACGACCAGTACT
GGCCCGAGGACCTGACCCAGAGCACCGGGGCCGAGACAAATGGAGGCCCAGATAGACAGGAGGGAGAGGA
AGGGCAGGGAGCCAGGAGTGCTCCTCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217018 representing NM_001162939
Red=Cloning site Green=Tags(s)

MVAGEVPESTQCPSLTSLNTKDVVRRRHKRRSRQHQRFMARKALLQEQELLSTAPGPGLCLLPSPSQMPA
VTEASDSRRQRPKARSGSNGLCSKKSVPREAPRPGPIKCVAIDCEMVGTGPQGRVSELARCSVVSYSGDV
LYDKYIRPEMPIVDYRTRWSGITRQHMHKAIPFQVAQKEILKLLKGKVVVGHALHNDFQALKYVHPRSQT
RDTTYVPNLLSQPSSLIRTRVSLKDLALNLLHKKIQVGHQGHSSVEDAMTAMELYQLVEVQWEQQVASSA
QAPAEDRGPDSSTDVEQYMDDQYWPEDLTQSTGAETNGGPDRQEGEEGQGARSAPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001162939
ORF Size 1008 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001162939.1, NP_001156411.1
RefSeq Size 2466 bp
RefSeq ORF 897 bp
Locus ID 68048
UniProt ID Q9CZI9
Cytogenetics 7 D2
Summary Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001162939" in other vectors (4)
SKU Description Size Price
MC210680 Aen (untagged) - Mouse apoptosis enhancing nuclease (Aen), transcript variant 2, (10ug) 10 ug
$330.00
MR217018 Aen (Myc-DDK-tagged) - Mouse apoptosis enhancing nuclease (Aen), transcript variant 2 10 ug
$503.00
MR217018L3 Lenti ORF clone of Aen (Myc-DDK-tagged) - Mouse apoptosis enhancing nuclease (Aen), transcript variant 2 10 ug
$803.00
MR217018L4 Lenti ORF clone of Aen (mGFP-tagged) - Mouse apoptosis enhancing nuclease (Aen), transcript variant 2 10 ug
$803.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.