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C1qtnf4 (NM_026161) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217017
C1qtnf4 (tGFP-tagged) - Mouse C1q and tumor necrosis factor related protein 4 (C1qtnf4), (10ug)
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ORF Plasmid:
tGFP DDK
  $530.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol C1qtnf4
Synonyms 0710001E10Rik; 9430004J15Rik; CTRP4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217017 representing NM_026161
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTGCTCTTGCTGGGCTTCCTAGGCCCGGCGGCCTGCTGGGCACTGGGCCCGGCTGGCCCTGGCT
CCTCGGAGCTGCGGTCAGCCTTCTCGGCGGCTCGCACCACCCCGCTGGAGGGCACGTCGGAGATGGCGGT
GACCTTCGACAAGGTGTACGTGAACATCGGGGGTGACTTCGACGCAGCCACCGGGCGGTTCCGCTGTCGC
GTGCCGGGCGCCTACTTCTTCTCCTTCACGGCCGGCAAGGCCCCGCACAAGAGCCTGTCGGTGATGCTGG
TGCGCAACCGCGACGAGGTGCAGGCGCTGGCTTTCGACGAGCAGCGACGGCCAGGCGCGCGGCGCGCGGC
CAGCCAGAGCGCCATGCTGCAGCTCGACTACGGCGACACGGTGTGGCTGCGGCTGCACGGCGCTCCGCAG
TACGCGCTCGGCGCGCCGGGCGCCACCTTCAGCGGCTACCTGGTGTACGCGGACGCCGACGCCGACGCGC
CTGCGCGCGGCCCCGCGGCCCCGGAGCCGCGCTCGGCCTTCTCCGCGGCGCGCACGCGCAGCCTGGTGGG
CTCGGACGCCGCCCCCGGCCCGCGCCACCGGCCGTTGGCCTTCGACACCGAGCTGGTAAACATAGGTGGC
GACTTCGACGCGGCGGCCGGCGTGTTCCGCTGCCGCCTGCCGGGAGCCTATTTCTTCTCCTTCACGCTGG
GCAAGCTGCCGCGCAAGACGCTGTCGGTGAAGCTGATGAAGAACCGCGACGAGGTGCAGGCCATGATTTA
CGACGACGGCGCTTCGAGGCGCCGTGAGATGCAGAGTCAGAGCGTGATGCTGCCGCTGCGGCGCGGCGAC
GCCGTCTGGCTACTTAGCCACGATCACGATGGCTATGGCGCCTACAGCAACCACGGCAAGTACATCACTT
TCTCAGGCTTCCTGGTGTACCCTGACCTCGCCGCCGCCGGCCCGCCGGCCCTCAAGCCCCCAGAGCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217017 representing NM_026161
Red=Cloning site Green=Tags(s)

MLLLLLGFLGPAACWALGPAGPGSSELRSAFSAARTTPLEGTSEMAVTFDKVYVNIGGDFDAATGRFRCR
VPGAYFFSFTAGKAPHKSLSVMLVRNRDEVQALAFDEQRRPGARRAASQSAMLQLDYGDTVWLRLHGAPQ
YALGAPGATFSGYLVYADADADAPARGPAAPEPRSAFSAARTRSLVGSDAAPGPRHRPLAFDTELVNIGG
DFDAAAGVFRCRLPGAYFFSFTLGKLPRKTLSVKLMKNRDEVQAMIYDDGASRRREMQSQSVMLPLRRGD
AVWLLSHDHDGYGAYSNHGKYITFSGFLVYPDLAAAGPPALKPPEL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026161
ORF Size 978 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026161.3, NP_080437.2
RefSeq Size 1383 bp
RefSeq ORF 981 bp
Locus ID 67445
UniProt ID Q8R066
Cytogenetics 2 E1
Summary May be involved in the regulation of the inflammatory network. The role as pro- or anti-inflammatory seems to be context dependent (By similarity). Seems to have some role in regulating food intake and energy balance when administered in the brain. This effect is sustained over a two-day period, and it is accompanied by decreased expression of orexigenic neuropeptides in the hypothalamus 3 h post-injection (Probable).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026161" in other vectors (2)
SKU Description Size Price
MC210555 C1qtnf4 (untagged) - Mouse C1q and tumor necrosis factor related protein 4 (C1qtnf4), (10ug) 10 ug
$330.00
MR217017 C1qtnf4 (Myc-DDK-tagged) - Mouse C1q and tumor necrosis factor related protein 4 (C1qtnf4) 10 ug
$330.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.