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Nek6 (NM_021606) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG217007
Nek6 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Nek6 or NM_021606"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nek6
Synonyms 1300007C09Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG217007 representing NM_021606
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGACAGCCCAGCCACATGCCTCATGGAGGGAGCCCAAACCACCTCTGCCATGCACTGGGGCCTG
CACCCCCTCCAGACCCACAGAGGCTCCCAAACACCCTGTCTTTTCGCTGCTCACTGGCAGACTTCCAAAT
TGAGAAGAAGATTGGCCGAGGACAGTTCAGTGAGGTTTACAAGGCCACTTGCCTGCTGGACAGGAAGACA
GTGGCTCTGAAGAAGGTGCAGATCTTTGAGATGATGGATGCCAAAGCCAGGCAAGACTGTGTCAAGGAAA
TTGGTCTCCTAAAGCAACTGAACCATCCGAATATCATCAAGTATCTGGACTCCTTCATCGAAGACAATGA
GCTGAACATTGTGCTGGAGTTGGCTGACGCGGGTGACCTCTCACAGATGATCAAGTATTTCAAGAAGCAG
AAGCGGCTTATCCCTGAGAGGACCGTGTGGAAGTACTTCGTGCAGCTGTGCAGCGCTGTGGAGCACATGC
ACTCCCGACGTGTGATGCACCGAGACATCAAGCCCGCCAACGTGTTCATCACAGCTACGGGCATTGTGAA
GCTTGGTGACCTCGGCCTGGGCCGCTTCTTCAGCTCGGAGACCACTGCGGCCCACTCACTAGTGGGAACA
CCATACTACATGTCACCCGAGAGAATCCATGAGAATGGCTACAACTTCAAGTCAGACATCTGGTCCCTAG
GCTGCCTGCTGTATGAGATGGCAGCTCTCCAGAGCCCCTTCTATGGAGATAAGATGAATCTCTTCTCCCT
CTGCCAAAAGATTGAGCAGTGTGACTACCCGCCTCTCCCGGGAGAGCACTACTCTGAGAAGCTTCGGGAA
CTGGTCAGTATGTGTATCTACCCTGACCCTGACCACCGACCTGACATTGTATATGTGCACCAGGTGGCTA
GACAGATGCACGTCTGGACATCCAGCACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG217007 representing NM_021606
Red=Cloning site Green=Tags(s)

MAGQPSHMPHGGSPNHLCHALGPAPPPDPQRLPNTLSFRCSLADFQIEKKIGRGQFSEVYKATCLLDRKT
VALKKVQIFEMMDAKARQDCVKEIGLLKQLNHPNIIKYLDSFIEDNELNIVLELADAGDLSQMIKYFKKQ
KRLIPERTVWKYFVQLCSAVEHMHSRRVMHRDIKPANVFITATGIVKLGDLGLGRFFSSETTAAHSLVGT
PYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLFSLCQKIEQCDYPPLPGEHYSEKLRE
LVSMCIYPDPDHRPDIVYVHQVARQMHVWTSST

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021606
ORF Size 939 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021606.3, NP_067619.1
RefSeq Size 3168 bp
RefSeq ORF 942 bp
Locus ID 59126
UniProt ID Q9ES70
Cytogenetics 2 24.41 cM
Summary Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, RPS6KB1 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (By similarity). Phosphorylates STAT3.UniProtKB/Swiss-Prot Function
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Resources
"NM_021606" in other vectors (4)
SKU Description Size Price
MG204452 Nek6 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6) 10 ug
$500.00
MR217007 Nek6 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6), transcript variant 2 10 ug
$289.00 $300.00
MR217007L3 Lenti ORF clone of Nek6 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6), transcript variant 2 10 ug
$600.00
MR217007L4 Lenti ORF clone of Nek6 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)-related expressed kinase 6 (Nek6), transcript variant 2 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.