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Arhgap28 (NM_172964) Mouse Tagged ORF Clone

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SKU
MG216999
Arhgap28 (tGFP-tagged) - Mouse Rho GTPase activating protein 28 (Arhgap28), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $868.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arhgap28
Synonyms AU044757; AW550892; E130310N06
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216999 representing NM_172964
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGAGGACTCGGGCGGCGTGGTGCTGACCGCCTACCACTCGCACGCGCGCTCGCAACCGCAGG
GCGCTGAGCCACGCTGCGCGTCCCGGGCCAGCCATCCGCTCAGCAGAAAATCCATTCCTCGCTGCCGACG
AATCAACAGGATGCTCTCCAATGAATCCCTGCATCCTCCCTCCTTTAGTCGCTCCAACTCTCAAGCCTCA
GTAGACAGCAGCGCATCCATGGAGGAGTTCCTGCGGGAAATTGAGAGTATCAAAGAGAGCAGCGTGGGGG
CGTCGCAGGAGCAGCCACCCACTGCTGCTGCTGCTGCTGCTGAAGTCAAGCCAGTGGATGAAGGAGAGCT
TGAAGCGGAGTGGCTACAAGATGTGGGATTGTCAACTCTGATCTCTGGCAACGAAGAGGAAGACGGTAAA
GCGCTCCTGTCTACATTGACTCGGACCCAAGCTGCTGCAGTGAAAAAGAGATACAACACATATACTCAGA
CTCTGAGGAAAAAGAATAAACAACCCGTCAGGGATGTCAGAGACATCTTCGGAGTCAGCGAATCTCCTCC
TAGTGATTCTTGTGAGCATGCTACTCAGTTGGACGGTACCAAGGAAGAAAAAGATCTGCCAGGAGTTACC
AAGACAAGCAGACCTCTGCCAGACGATGCTTCTCTCAGTAGTACCACCCTATCCAATGGCGCCCAGGATG
AAGAAGGTGGTTTTGTGGCCCTACAGAGTGGTTCTGTGTCAATACTTGAGGCTATCCCGGATATTGCTGT
TCACACCAATGGATCTGCAGATGCTGAGCAGTCGGTTCAGAGCACATTGAGTGATGATGATTATCACGGG
AAGAACGTTCCAGCAGAGGCTGAAGAGCTGTCCTTTGAGGTCTCTTACTCAGAAATGGTGACAGAAATGC
CTGATAGAAATAAATGGAAGAAGTCGGACATTAAGAAAGAAGACTATGTCTTAACTAAATTTATCATTCA
GAAAACAAGATTTGGGTTGACCGAGACGGGGGACCTGTCTGTGGAAGACATGAAGAAAATCCGCCACCTC
TCTCTGATTGAGCTGACAGCCTTCTTCGATGCTTTTGGGATACAACTGAAGAGAAACAAAACCGAGCGAG
TGAGAGGCCGAGACAACGGGATTTTCGGAGTGCCACTTACAGTCCTCCTGGACAATGACCGGAAGAAGGA
CCCTGCAGTGAAAGTTCCCCTGGTGTTACAAAAATTTTTTCAGAAAGTCGAGGAATCAGGTCTGGAATCG
GAAGGAATTTTTCGACTCTCGGGATGTACTGCCAAAGTCAAGCAGTACCGCGAAGAACTGGATGCCAGGT
TCAATGCTGACAAGTTCAAGTGGGACAAGATGTGTCACAGAGAAGCTGCGGTCATGTTGAAAGCATTCTT
CAGAGAGCTGCCCACCTCTCTCTTCCCTGTTGAATATATACCCGCCTTCATCTCTCTGATGGAAAGAGGA
CCTGACATCAAAGTGCAGTTTCAAGCCTTGCACCTCATGGTCATGGCCCTGCCTGATGCCAACAGGGACA
CAGCGCAGGCTCTGATGGCATTCTTCAATAAAGTGATCGCCAACGAATCCAAGAACCGCATGAACCTGTG
GAACATTTCCACAGTGATGGCGCCCAACTTGTTCTTCAGCAGAAGCAAGCACTCTGACTGCGAGGAACTG
CTGCTGGCCAACACGGCGACCCACATCATCCGCCTGATGCTGAAGTACCAGAAGATTCTGTGGAAGGTTC
CATCTTTCTTGATCACCCAGGTCAGAAGGATGAATGAGGCCACCATGCTGTTGAAGAAGCAGCTCCCGAG
TATGAAGAAGCTGCTCAGGAGGAAGACCCTGGATCGAGAGGTTTCAATCCTCAAGACCTCAAAGGTACCA
CAAAAATCACCTTCCTCAAGAAGAATGTCTGATGTGCCAGAAGGTGTCATAAGGGTCCACGCTCCACTTC
TGTCCAAAGTGTCCATGGCCATTCAGCTCAACAGTCAGACCAAAGCCAAAGATATCCTGGCGAAATTTCA
GTATGAGAACAGTCATGGTTCATCTGAACATATTAAGATGCAGAACCAAAGGTTATATGAAGTTGGAGGA
AATATAGGACAACACTGTTTAGATCCTGATGCATATATATTGGATGTGTATCATATAAATCCTCATGCAG
AATGGGTCATCAAGCCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216999 representing NM_172964
Red=Cloning site Green=Tags(s)

MEVEDSGGVVLTAYHSHARSQPQGAEPRCASRASHPLSRKSIPRCRRINRMLSNESLHPPSFSRSNSQAS
VDSSASMEEFLREIESIKESSVGASQEQPPTAAAAAAEVKPVDEGELEAEWLQDVGLSTLISGNEEEDGK
ALLSTLTRTQAAAVKKRYNTYTQTLRKKNKQPVRDVRDIFGVSESPPSDSCEHATQLDGTKEEKDLPGVT
KTSRPLPDDASLSSTTLSNGAQDEEGGFVALQSGSVSILEAIPDIAVHTNGSADAEQSVQSTLSDDDYHG
KNVPAEAEELSFEVSYSEMVTEMPDRNKWKKSDIKKEDYVLTKFIIQKTRFGLTETGDLSVEDMKKIRHL
SLIELTAFFDAFGIQLKRNKTERVRGRDNGIFGVPLTVLLDNDRKKDPAVKVPLVLQKFFQKVEESGLES
EGIFRLSGCTAKVKQYREELDARFNADKFKWDKMCHREAAVMLKAFFRELPTSLFPVEYIPAFISLMERG
PDIKVQFQALHLMVMALPDANRDTAQALMAFFNKVIANESKNRMNLWNISTVMAPNLFFSRSKHSDCEEL
LLANTATHIIRLMLKYQKILWKVPSFLITQVRRMNEATMLLKKQLPSMKKLLRRKTLDREVSILKTSKVP
QKSPSSRRMSDVPEGVIRVHAPLLSKVSMAIQLNSQTKAKDILAKFQYENSHGSSEHIKMQNQRLYEVGG
NIGQHCLDPDAYILDVYHINPHAEWVIKP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172964
ORF Size 2187 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172964.4, NP_766552.3
RefSeq Size 5317 bp
RefSeq ORF 2190 bp
Locus ID 268970
UniProt ID Q8BN58
Cytogenetics 17 E1.1-E1.2
Summary GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172964" in other vectors (4)
SKU Description Size Price
MC221202 Arhgap28 (untagged) - Mouse Rho GTPase activating protein 28 (Arhgap28), (10ug) 10 ug
$735.00
MR216999 Arhgap28 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 28 (Arhgap28) 10 ug
$589.00 $668.00
MR216999L3 Lenti ORF clone of Arhgap28 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 28 (Arhgap28) 10 ug
$968.00
MR216999L4 Lenti ORF clone of Arhgap28 (mGFP-tagged) - Mouse Rho GTPase activating protein 28 (Arhgap28) 10 ug
$968.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.