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Otop2 (NM_172801) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216988
Otop2 (tGFP-tagged) - Mouse otopetrin 2 (Otop2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $724.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Otop2
Synonyms 4732464P15
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216988 representing NM_172801
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGAGGAACTGGTCCCGCATCCCAATGAGAGCCTGCCGGGGCCTCGGGCCAGCCCCCGTGAGGTGT
GGAAAAAGGGCGGCCGCCTGCTGTCGGTGCTGTTGGCTGTCAACGTGCTGCTGCTCGCCTGCACGCTCAT
CAGCGGCGGTGCCTTCAACAAGGTGGCGGTGTATGACACGGACGTGTTCGCTCTGCTCACCACCATGATG
CTGCTGGCCGCGCTATGGATAGTCTTCTACCTCCTCCGCACCGCTCGCTGTCCAGACGCGGTGCCTTACC
GGGATGCTCATGCTGGTCCCATCTGGCTCCGAGGTGGGCTGGTGCTGTTTGGAATCTGCACCCTTGTCAT
GGATGTCTTCAAGACTGGCTACTATTCCAGTTTCTTTGAGTGCCAGTCGGCCATCAAGATCCTGCACCCC
ATCATCCAAGCCGTGTTTGTCATTGTCCAGACTTACTTTCTCTGGATCTCCGCTAAAGACTGTATCCATA
CCCACCTGGACCTGACCCGGTGTGGGCTCATGTTCACCCTCGCCACCAACCTCGCCATCTGGATGGCAGC
CGTAGTGGATGAGTCCGTGCACCAGGCCCACTCCTACAGTGGTTCCCATGGCAACACCAGCCACACCCGC
CTCAACCCTGACTCAAAGCGTGCAGGAGGTGCAGCTGAAGAAGACCCGTGCCTCTGCAGCACAGCCATCT
GTCAGATCTTCCAGCAAGGCTATTTCTACCTGTATCCCTTCAACATCGAGTACAGCCTCTTTGCTTCCAC
CATGCTGTATGTCATGTGGAAGAATGTGGGAAGGCTGCTCGCCTCCACCCACGGCCACGGCCACACACCG
TCCAGGGTCAGCCTGTTCCGGGAGACCTTCTTTGCCGGCCCAGTCTTGGGCCTGCTCCTCTTTGTGGTGG
GGTTGGCTGTCTTCATCCTTTATGAGGTCCAGGTGAGTGGAGAGAGAGGCCACACCCGACAGGCCCTGGT
CATCTACTACAGCTTTAACATTGTCTGTCTGGGGCTCATGACTCTGGTCAGCCTGAGTGGTTCAGTCATC
TATCGTTTTGACCGTCGGGCCATGGACCATCATAAGAACCCGACACGGACCCTGGATGTGGCCCTGCTAA
TGGGCGCCGCCCTGGGCCAATACGCTATCTCCTACTACTCTATCGTGGCTGTGGTGGTGGGCTCTCCCAG
AGACCTGCAGGGGGCGCTCAACCTGTCCCACGCTCTACTCATGATCGCTCAGCACACCTTCCAGAATGTC
TTCATCATTGAGAGCCTCCATCGGGGACCTCCTGGGGCTGAGCCTCGTGAAATGCCCCCCAAGGAGCCCT
GCCAAGGCATCACCTTTGCTAACCTGGATGCTATCCGCACCTTGCCTTCCTGTCCACCCACCCCCAGGCT
GGTCATTCCCAACCTAGAAAGTCCTCAGGAAGCAGTGGCTATCATCTCGGCCCCCAGGTGCCACTGGCGA
CGTCGGTGCCTGAAAGACATCTCTCTGTTTCTCCTGCTCTGTAACGTCATCCTGTGGATCATGCCTGCCT
TTGGCGCTCGCCCGCACTTCAGCAATACCGTGGAGGTGGACTTTTACGGTTACTCCCTCTGGGCGGCCAT
CGTCAACATCTGCCTACCTTTTGGCATCTTCTACCGAATGCACGCTGTCTCCAGTCTACTGGAGGTCTAT
GTACTGTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216988 representing NM_172801
Red=Cloning site Green=Tags(s)

MSEELVPHPNESLPGPRASPREVWKKGGRLLSVLLAVNVLLLACTLISGGAFNKVAVYDTDVFALLTTMM
LLAALWIVFYLLRTARCPDAVPYRDAHAGPIWLRGGLVLFGICTLVMDVFKTGYYSSFFECQSAIKILHP
IIQAVFVIVQTYFLWISAKDCIHTHLDLTRCGLMFTLATNLAIWMAAVVDESVHQAHSYSGSHGNTSHTR
LNPDSKRAGGAAEEDPCLCSTAICQIFQQGYFYLYPFNIEYSLFASTMLYVMWKNVGRLLASTHGHGHTP
SRVSLFRETFFAGPVLGLLLFVVGLAVFILYEVQVSGERGHTRQALVIYYSFNIVCLGLMTLVSLSGSVI
YRFDRRAMDHHKNPTRTLDVALLMGAALGQYAISYYSIVAVVVGSPRDLQGALNLSHALLMIAQHTFQNV
FIIESLHRGPPGAEPREMPPKEPCQGITFANLDAIRTLPSCPPTPRLVIPNLESPQEAVAIISAPRCHWR
RRCLKDISLFLLLCNVILWIMPAFGARPHFSNTVEVDFYGYSLWAAIVNICLPFGIFYRMHAVSSLLEVY
VLS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172801
ORF Size 1689 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172801.2, NP_766389.2
RefSeq Size 2350 bp
RefSeq ORF 1692 bp
Locus ID 237987
UniProt ID Q80SX5
Cytogenetics 11 E2
Summary Proton-selective channel that specifically transports protons into cells. Proton-selective channel activity is probably required in cell types that use changes in intracellular pH for cell signaling or to regulate biochemical or developmental processes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172801" in other vectors (4)
SKU Description Size Price
MC219129 Otop2 (untagged) - Mouse otopetrin 2 (Otop2), (10ug) 10 ug
$577.00
MR216988 Otop2 (Myc-DDK-tagged) - Mouse otopetrin 2 (Otop2) 10 ug
$289.00 $524.00
MR216988L3 Lenti ORF clone of Otop2 (Myc-DDK-tagged) - Mouse otopetrin 2 (Otop2) 10 ug
$824.00
MR216988L4 Lenti ORF clone of Otop2 (mGFP-tagged) - Mouse otopetrin 2 (Otop2) 10 ug
$824.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.