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Tmem8 (NM_021793) Mouse Tagged ORF Clone

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SKU
MG216983
Tmem8 (tGFP-tagged) - Mouse transmembrane protein 8 (five membrane-spanning domains) (Tmem8), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $904.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Tmem8 or NM_021793"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem8
Synonyms AW490096; GPI-PLA2; M83; Pgap6; Tmem8a
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216983 representing NM_021793
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCGGGTTGGGGCCGGGGGCACAGCGCGGGAGGCAGCGACCGGGTCACTGCTGCTGCTGCTGCTGC
TCCTGGCTCGGCCTCCGCCTGCCGCCGCCAGCAACAGCAAGGAGAGCGAGGCTGGACTGGTATCTGAACA
CTTCTCCCAGGCCCCACAGAAGCTGTCCTTCTACAGCTGGTATGGCAGCACCCGGCTCTTCCACTTCCGC
GTGCCCCCGGACACTGTGCTGCTCCGCTGGCTGCTGCACGTGTCCCAGGGAAGTCCCTCATGCACTGACG
AAGAGATCACAGTGCATTTCCGCTATGGCGCCCCTCCTGTCATCAACCCTCTGGGCACCAGCTTCCCTGA
CAACACCTTGAGCCACGCCTCCTTCCATATCAGGGCATTGTTGAGCACCTTGATGCTGGACAACACCTCA
GTCAACATCTCCCACCCTGCCCCTGGGGACTGGTTCCTGGTTGCTCACCTCCCTCCCTCCTCCCAGAAGA
TCCAGGTGAAGGGCTTTGTTCCAACCTGTGCCTACATCTTCCAGCCTGATATGCTGGTAATGAGGGTGGT
TGAAGTCTCCACCCTAGAGCCTGATGTTCCCCTCCCACAGACGCTGCTCTCCTACCCCAGCTACCTCAAG
ATCTTTGTCCCTGAATACACCCAGGAGCTTCGGTTGGAACTGCAGGGCTGTGTGTCCAGTGTGAGCCCCG
GCTGCCCAGTACGTGTCACAGTGGGGGCAACCACCCTGCCTAGAAACTTCCAGAGGGTGCTGACTTGCAC
TGGCCTCGCTCCATCCTGCCATTTGCTACTGTCCTCTCCACCCTGGGGCCGGTGGTTACAAGTGACATTT
GAGAGCCTGGCTGAACCTCATGTGACAGTGGGCTTTACTGCTAAAGCTGTCTTTACAGTATGTAGGCCAT
GGAGTGTGACCATCCATCATCTTATACAAAACAACCCAAACCAGACTTATGATACCTCTGCAATCCAGCT
GTCCCAAAGCGCTGTCCACCGGGACCTGGGCAGGAGTAGCAGGGTGGACAGTGGCCCCTTCTGTCTCCTG
AACTATCCAGTCCTGAGGGAGGACACAGATGTGGTGTCTGTACACTTCCAGCCCCTGAATGGGGCCTTCG
TGCTAGTGCATTCAAGCATGCCTTCTGTAATGCAGCTCCGCCTTGATACAGGCATGGACAGCGGAGGCTC
CTTCATCATCGTCCTGAGGACTAACAAGACAGAGGTCACAAATGGCACCTTGGTAGCAGCTTGTGTGAAT
GCTGCCTCCCCTTTCCTCAGCTTCAACACTTCACTCAACTGCACCACAGCCTTTTTCCAGGGCTACCCCA
TGTTTCTGAGAGCCTCATCTCACATGGCCAACCTCATCATGCCCTTCCCGGAGACAGACAACTGGTACCT
CTCCCTGCAGCTTGTGTGCCCTGAGAGTCCTGAGGATTGTGAACAGGCTGTGGTTCGCGTGGAGACCATC
TTGTATCTGGTGCCCTGTTTGAATGACTGTGGGCCCTACGGCCAGTGTCTCCTGCTCCGTAGATACGGCT
ATGTGTACGCAGGCTGCAGCTGCAAGGCAGGTTGGCGTGGGTGGAGCTGCACCGACAACAGCACAGCGCA
GACCGTAGCCCAGCAGAGGGCAGCAGCGCTTCTGCTCACGCTCAGCAACCTCATGTTCCTGGCCCCCATT
GCTATATCCCTGCACCGTTCCTTCCTGGTCGAGGCCTCTGTCTACTTTTACACCATGTTCTTCTCCACGT
TCTACCACGCCTGTGACCAACCCGGGGAGGCAGTGTTGTGCATCCTCAGTTATGACACGCTACAGTATTG
CGACTTTCTGGGCTCTGGGGCGTCCACCTGGGTGACCATTCTTTGCATGGCCCGTCTGAAGACAATCCTG
AAACAGGTACTACTTGTCCTCGGCACACTCGTCATCGCCATGTCCTTGCAGATGGATCGCAGGGGCATCT
GGAACTTGATGGGACCTTGCGTCTTTGCTTTTGTGATCATGGCCTCCATGTGGATATACCGCTGTGGGCA
CCGGGGACAGTGCTACCCAACCTCCTGGCAGCGCTGGGTCTTCTACCTCCTGCCTGGCATCTCCATGGCT
TCCGTTGGCATCGCCATGTATACTTCAATGATGACCAGTGACAATTACTACTACACCCACAGCATTTGGC
ACATCCTACTGGCTGGTAGTGCAGCATTTCTTCTGCCACCACGAGAGGAAAAAGCTGGGTCCTGGGCCTG
TTTGCAGAAGTTCCCTTGTCACTACCAGATCTGCAGGAATGATCGGGATGAGTTGTACACAGTGACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216983 representing NM_021793
Red=Cloning site Green=Tags(s)

MGRVGAGGTAREAATGSLLLLLLLLARPPPAAASNSKESEAGLVSEHFSQAPQKLSFYSWYGSTRLFHFR
VPPDTVLLRWLLHVSQGSPSCTDEEITVHFRYGAPPVINPLGTSFPDNTLSHASFHIRALLSTLMLDNTS
VNISHPAPGDWFLVAHLPPSSQKIQVKGFVPTCAYIFQPDMLVMRVVEVSTLEPDVPLPQTLLSYPSYLK
IFVPEYTQELRLELQGCVSSVSPGCPVRVTVGATTLPRNFQRVLTCTGLAPSCHLLLSSPPWGRWLQVTF
ESLAEPHVTVGFTAKAVFTVCRPWSVTIHHLIQNNPNQTYDTSAIQLSQSAVHRDLGRSSRVDSGPFCLL
NYPVLREDTDVVSVHFQPLNGAFVLVHSSMPSVMQLRLDTGMDSGGSFIIVLRTNKTEVTNGTLVAACVN
AASPFLSFNTSLNCTTAFFQGYPMFLRASSHMANLIMPFPETDNWYLSLQLVCPESPEDCEQAVVRVETI
LYLVPCLNDCGPYGQCLLLRRYGYVYAGCSCKAGWRGWSCTDNSTAQTVAQQRAAALLLTLSNLMFLAPI
AISLHRSFLVEASVYFYTMFFSTFYHACDQPGEAVLCILSYDTLQYCDFLGSGASTWVTILCMARLKTIL
KQVLLVLGTLVIAMSLQMDRRGIWNLMGPCVFAFVIMASMWIYRCGHRGQCYPTSWQRWVFYLLPGISMA
SVGIAMYTSMMTSDNYYYTHSIWHILLAGSAAFLLPPREEKAGSWACLQKFPCHYQICRNDRDELYTVT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021793
ORF Size 2307 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021793.2, NP_068565.1
RefSeq Size 3480 bp
RefSeq ORF 2310 bp
Locus ID 60455
UniProt ID Q9ESN3
Cytogenetics 17 A3.3
Summary Involved in the lipid remodeling steps of GPI-anchor maturation. Lipid remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchor proteins (GPI-AP). Has phospholipase A2 activity that removes an acyl-chain at the sn-2 position of GPI-anchors during the remodeling of GPI. Required for the shedding of the GPI-AP TDGF1, but not CFC1, at the cell surface. Shedding of TDGF1 modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. Also indirectly involved in the translocation of RAC1 from the cytosol to the plasma membrane by maintaining the steady state amount of CAV1-enriched plasma membrane subdomains, stabilizing RAC1 at the plasma membrane.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_021793" in other vectors (3)
SKU Description Size Price
MR216983 Tmem8 (Myc-DDK-tagged) - Mouse transmembrane protein 8 (five membrane-spanning domains) (Tmem8) 10 ug
$589.00 $704.00
MR216983L3 Lenti ORF clone of Tmem8 (Myc-DDK-tagged) - Mouse transmembrane protein 8 (five membrane-spanning domains) (Tmem8) 10 ug
$1,004.00
MR216983L4 Lenti ORF clone of Tmem8 (mGFP-tagged) - Mouse transmembrane protein 8 (five membrane-spanning domains) (Tmem8) 10 ug
$1,004.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.