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Nagk (NM_019542) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216982
Nagk (tGFP-tagged) - Mouse N-acetylglucosamine kinase (Nagk) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
  $703.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nagk
Synonyms Gnk
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216982 representing NM_019542
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCGCTTTATGGTGGCGTGGAAGGGGGAGGCACACGGTCCAAAGTCCTTTTACTTTCTGAGGATG
GGCAGATCCTGGCAGAAGCAGATGGACTGAGCACAAACCACTGGCTGATTGGCACAGACCAGTGTGTGGA
GAGGATCAATGAGATGGTGGACAGGGCCAAACAGAAGGCTGGAGTGGATCCTCTGGTGCCCCTTCGAAGC
CTGGGCCTGTCCCTGAGTGGTGGGGAGCAGGAGGATGCAGTGAGGCTCCTGATTGAGGAGTTGAGGCACC
GCTTTCCCAACCTGAGTGAAAACTACTTAATCACCACGGATGCAGCAGGTTCCATCGCCACAGCCACACC
GGACGGTGGGATTGTGCTCATCTCTGGAACAGGCTCCAACTGTAGGCTTATCAACCCTGATGGCTCCGAG
AGTGGCTGTGGAGGCTGGGGCCACATGATGGGAGACGAGGGCTCAGCCTACTGGATTGCACACCAAGCTG
TGAAAATTGTGTTTGATTCCATCGACAACCTGGAGGCAGCTCCTCATGATATTGGCCATGTCAAGCAGGC
CATGTTCGACTATTTCCAGGTGCCAGATCGGCTTGGAATCCTCACCCATTTGTATAGGGACTTTGATAAA
TGCAAGTTTGCTGGATTTTGCCAGAAAATTGCAGAAGGTGCACATCAGGGAGACCCTCTTTCCAGGTACA
TCTTCAGGAAGGCTGGAGAGATGCTGGGCAGACATGTTGTGGCAGTATTGCCAGAGATTGACCCGGTTTT
GTTCCAAGGGGAGCTTGGCCTCCCCATTCTGTGTGTGGGCTCAGTGTGGAAGAGCTGGGAGCTCCTGAAG
GAAGGCTTTCTCCTGGCGCTGACGCTGGGCCGAGAGCAGCAGGCACAGAACTCCTTCTCCAGCTTCACCC
TGATGAAGTTGCGGCATTCTTCTGCATTGGGGGGTGCCAGCCTGGGGGCCAGGCACATTGGATACCACCT
TCCCATGGACTACAGTATCAATGCCATTGCCTTCTATTCCTATACCTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216982 representing NM_019542
Red=Cloning site Green=Tags(s)

MAALYGGVEGGGTRSKVLLLSEDGQILAEADGLSTNHWLIGTDQCVERINEMVDRAKQKAGVDPLVPLRS
LGLSLSGGEQEDAVRLLIEELRHRFPNLSENYLITTDAAGSIATATPDGGIVLISGTGSNCRLINPDGSE
SGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNLEAAPHDIGHVKQAMFDYFQVPDRLGILTHLYRDFDK
CKFAGFCQKIAEGAHQGDPLSRYIFRKAGEMLGRHVVAVLPEIDPVLFQGELGLPILCVGSVWKSWELLK
EGFLLALTLGREQQAQNSFSSFTLMKLRHSSALGGASLGARHIGYHLPMDYSINAIAFYSYTF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019542
ORF Size 1029 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019542.2, NP_062415.1
RefSeq Size 1330 bp
RefSeq ORF 1032 bp
Locus ID 56174
UniProt ID Q9QZ08
Cytogenetics 6 C3
Summary Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019542" in other vectors (5)
SKU Description Size Price
MC200142 Nagk (untagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 1, (10ug) 10 ug
$457.00
MG205154 Nagk (tGFP-tagged) - Mouse N-acetylglucosamine kinase (Nagk) 10 ug
$657.00
MR216982 Nagk (Myc-DDK-tagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 1 10 ug
$503.00
MR216982L3 Lenti ORF clone of Nagk (Myc-DDK-tagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 1 10 ug
$803.00
MR216982L4 Lenti ORF clone of Nagk (mGFP-tagged) - Mouse N-acetylglucosamine kinase (Nagk), transcript variant 1 10 ug
$803.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.