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Upf1 (NM_001122829) Mouse Tagged ORF Clone

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SKU
MG216969
Upf1 (tGFP-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,178.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Upf1 or NM_001122829"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Upf1
Synonyms B430202H16Rik; NORF1; PNORF-1; Rent1; Upflp
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216969 representing NM_001122829
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGTGGAGGCGTACGGCCCCAGCTCGCAAACACTCACCTTCTTGGACACTGAGGAGGCCGAGCTGC
TCGGCGCCGACACCCAGGGCTCCGAGTTCGAATTCACCGACTTCACCCTTCCCAGCCAGACGCAGACGCC
CCCCGGCGGCCCCGGCGGCGCGGGAGGCCCGGGCGGAGCGGGCGCAGGCGGCGCGGCCGGCCAGCTCGAC
GCACAAGTTGGACCAGAGGGCATCTTGCAAAATGGGGCTGTGGATGACAGTGTGGCCAAGACCAGCCAGC
TGCTAGCTGAGCTGAACTTCGAGGAAGATGAAGAGGACACATACTACACTAAGGACCTCCCAGTCCACGC
CTGCAGTTACTGTGGAATCCATGATCCTGCCTGCGTGGTTTACTGTAATACCAGCAAGAAGTGGTTCTGC
AATGGCCGAGGAAATACTTCTGGCAGCCACATTGTGAATCACCTCGTGAGGGCAAAATGCAAGGAAGTGA
CGCTGCACAAGGACGGGCCTCTGGGCGAGACCGTGCTGGAGTGCTACAACTGTGGCTGCCGCAACGTCTT
CCTGCTGGGCTTCATCCCTGCGAAGGCCGACTCTGTGGTGGTGCTGTTGTGCAGGCAGCCCTGTGCCAGC
CAGAGCAGCCTGAAGGACATCAACTGGGACAGCTCACAGTGGCAGCCCCTAATCCAGGACCGGTGCTTTC
TGTCATGGCTGGTCAAGATTCCCTCTGAGCAGGAGCAGCTGCGAGCACGGCAGATCACGGCACAGCAGAT
CAACAAGCTGGAAGAGCTCTGGAAGGAAAATCCTTCAGCCACTCTGGAGGACCTGGAGAAGCCAGGCGTA
GACGAGGAGCCACAGCACGTGCTCCTGCGTTACGAGGATGCTTACCAGTACCAGAACATCTTCGGGCCAC
TGGTCAAGCTGGAGGCTGACTATGACAAGAAGTTGAAGGAGTCACAGACTCAAGATAACATCACGGTCAG
GTGGGACCTGGGCCTTAACAAGAAGAGAATCGCCTTCTTCACTTTGCCCAAGACTGACTCTGGTAATGAG
GATTTAGTCATAATTTGGTTAAGAGACATGCGGCTCATGCAGGGTGATGAGATCTGTCTGCGGTACAAAG
GGGATCTGGCGCCCCTGTGGAAGGGGATTGGCCACGTCATCAAGGTTCCTGATAATTATGGTGATGAGAT
TGCTATTGAGCTCCGCAGCAGCGTGGGTGCCCCTGTGGAAGTGACCCACAACTTCCAAGTGGATTTTGTG
TGGAAGTCAACCTCTTTTGATAGGATGCAGAGTGCACTGAAGACCTTCGCTGTGGACGAGACCTCTGTGT
CAGGGTATATTTACCACAAGCTGCTGGGCCACGAGGTGGAGGATGTGGTCATCAAGTGCCAGCTGCCAAA
GCGCTTCACAGCTCAGGGGCTCCCTGACCTCAACCACTCTCAGGTGTATGCTGTGAAGACCGTGCTGCAG
AGACCACTCAGCCTCATCCAGGGCCCTCCAGGCACAGGCAAGACTGTGACATCAGCCACTATTGTCTACC
ACCTTGCTCGGCAGGGCAATGGGCCTGTACTGGTTTGTGCTCCAAGTAACATCGCTGTGGACCAGCTCAC
AGAGAAGATCCACCAGACAGGACTGAAGGTCGTACGCCTCTGTGCCAAGAGCCGTGAGGCCATTGACTCC
CCAGTGTCCTTCCTGGCTTTGCACAACCAGATCAGGAACATGGACAGCATGCCTGAGCTGCAGAAGCTGC
AGCAGCTAAAGGATGAGACAGGCGAGCTGTCATCTGCAGATGAGAAGCGGTACCGGGCGCTTAAGCGCAC
AGCTGAGAGAGAACTTCTCATGAATGCAGATGTCATATGCTGCACATGTGTGGGTGCTGGTGACCCGAGG
CTGGCCAAGATGCAGTTCCGTTCCATCCTCATTGACGAGAGCACCCAGGCCACTGAGCCTGAGTGCATGG
TGCCTGTAGTCCTTGGGGCCAAGCAGCTAATCCTCGTCGGTGACCACTGCCAGCTGGGCCCAGTGGTGAT
GTGCAAGAAGGCAGCCAAGGCCGGACTGTCACAATCGCTCTTCGAGCGCTTGGTGGTGCTGGGCATCCGG
CCCATCCGCCTGCAGGTGCAATACCGCATGCACCCTGCACTCAGCGCCTTTCCGTCCAACATCTTCTACG
AGGGCTCATTGCAGAATGGCGTCACTGCAGCGGATCGTGTCAAAAAAGGCTTTGACTTCCAGTGGCCACA
ACCTGACAAGCCTATGTTCTTCTACGTGACGCAGGGCCAGGAGGAGATTGCCAGCTCTGGCACATCCTAC
CTCAACAGGACGGAGGCAGCCAATGTGGAGAAGATAACTACGAAGCTGTTGAAGGCAGGTGCAAAGCCTG
ACCAGATCGGCATCATCACCCCCTACGAGGGCCAGCGCTCTTACTTGGTGCAGTACATGCAGTTCAGCGG
CTCCCTGCACACAAAGCTCTACCAGGAAGTGGAAATTGCCAGTGTGGACGCCTTCCAGGGCCGGGAGAAG
GACTTCATCATTCTGTCCTGCGTGCGCGCCAATGAACATCAGGGCATTGGGTTCCTAAACGACCCCCGGC
GTCTGAATGTGGCTCTCACCAGAGCAAGATATGGCGTGATCATTGTGGGTAACCCAAAGGCCCTGTCGAA
GCAGCCCCTGTGGAATCACCTGCTGAGCTACTACAAGGAACAGAAGGCGCTAGTGGAAGGGCCGCTCAAC
AACCTACGTGAGAGCCTCATGCAGTTCAGCAAGCCTCGCAAACTTGTCAACACTGTCAACCCGGGTGCCC
GCTTCATGACTACTGCCATGTACGATGCCCGTGAGGCCATCATCCCCGGGTCTGTCTATGACCGCAGCAG
CCAGGGCCGGCCCTCGAACATGTACTTCCAGACCCATGACCAGATTAGTATGATCAGCGCAGGCCCCAGC
CACGTGGCTGCCATGAACATCCCTATTCCCTTCAACTTGGTCATGCCTCCCATGCCGCCACCTGGCTACT
TCGGACAGGCCAACGGGCCGGCAGCTGGTCGGGGCACCCCAAAAACCAAGACTGGCCGTGGGGGCCGCCA
GAAGAACCGCTTTGGGCTTCCTGGGCCCAGCCAGACCACCCTTCCCAACAGCCAGGCCAGCCAGGACGTG
GCCTCCCAGCCCTTTTCACAGGGTGCCCTCACACAGGGTTACGTGTCCATGAGCCAGCCCTCTCAGATGA
GCCAGCCTGGCCTCTCCCAGCCAGAACTGTCCCAGGACAGCTACCTCGGTGATGAGTTTAAATCACAGAT
TGACGTGGCACTCTCACAAGACTCCACATACCAGGGAGAGCGGGCATACCAGCACGGCGGGGTCACCGGG
CTGTCCCAGTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216969 representing NM_001122829
Red=Cloning site Green=Tags(s)

MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGAGGPGGAGAGGAAGQLD
AQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFC
NGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCAS
QSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGV
DEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAFFTLPKTDSGNE
DLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFV
WKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVVIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQ
RPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDS
PVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPR
LAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIR
PIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSY
LNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREK
DFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLSYYKEQKALVEGPLN
NLRESLMQFSKPRKLVNTVNPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQISMISAGPS
HVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKTKTGRGGRQKNRFGLPGPSQTTLPNSQASQDV
ASQPFSQGALTQGYVSMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTG
LSQY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001122829
ORF Size 3372 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001122829.2, NP_001116301.1
RefSeq Size 4631 bp
RefSeq ORF 3375 bp
Locus ID 19704
UniProt ID Q9EPU0
Cytogenetics 8 34.15 cM
Summary RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (By similarity). Essential for embryonic viability.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001122829" in other vectors (4)
SKU Description Size Price
MC223643 Upf1 (untagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 1, (10ug) 10 ug
$1,077.00
MR216969 Upf1 (Myc-DDK-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 1 10 ug
$589.00 $978.00
MR216969L3 Lenti ORF clone of Upf1 (Myc-DDK-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 1 10 ug
$1,278.00
MR216969L4 Lenti ORF clone of Upf1 (mGFP-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 1 10 ug
$1,278.00

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