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Idh3g (NM_008323) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216960
Idh3g (tGFP-tagged) - Mouse isocitrate dehydrogenase 3 (NAD+) gamma (Idh3g) nuclear gene encoding mitochondrial protein, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Idh3g
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216960 representing NM_008323
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGAAGGTGGCGATAGCTGCTGGCGGTGCTGCAAAGGCAATGCTCAAGCCAACTCTCCTCTGCC
GTCCTTGGGAGGTTCTGGCTGCCCATGTGGCCCCCCGAAGGAGCATTTCCTCACAACAAACAATTCCTCC
ATCTGCTAAGTATGGTGGGCGGCATACAGTGACTATGATCCCAGGGGATGGCATCGGCCCAGAGCTCATG
TTGCATGTTAAGTCTGTATTCAGGCATGCATGTGTGCCGGTGGACTTTGAAGAGGTGCATGTAAGCTCCA
ACGCTGATGAGGAGGACATCCGCAATGCCATCATGGCCATCCGCCGGAACCGTGTGGCCCTGAAGGGCAA
CATTGAAACAAATCATAACCTGCCACCATCCCACAAATCTCGAAACAACATCCTTCGCACCAGCCTAGAC
CTCTATGCCAACGTCATCCACTGTAAGAGCCTGCCAGGAGTGGTGACCCGGCACAAGGACATAGACATCC
TCATTGTACGGGAAAACACAGAAGGCGAGTACAGCAGCCTGGAGCATGAGAGCGTAGCAGGAGTGGTGGA
GAGCTTGAAGATTATCACCAAAGCCAAGTCCCTGCGCATTGCTGAATATGCTTTCAAGCTGGCCCAGGAG
AGTGGGCGTAAGAAAGTGACGGCTGTGCACAAGGCCAACATCATGAAACTGGGTGATGGACTCTTCCTCC
AGTGCTGCAGGGAAGTAGCAGCCCACTACCCTCAGATCACCTTTGACAGCATGATTGTAGACAACACAAC
AATGCAGCTGGTATCCCGGCCTCAGCAGTTTGATGTCATGGTGATGCCTAATCTCTATGGTAACATTGTC
AACAACGTCTGTGCAGGGCTAGTTGGAGGCCCAGGCCTTGTGGCTGGGGCCAACTATGGCCATGTGTATG
CAGTATTCGAGACAGCTACAAGGAACACAGGCAAAAGTATTGCCAATAAGAACATTGCTAACCCGACTGC
CACACTGCTAGCAAGCTGCATGATGCTAGACCACCTCAAGCTCCACTCCTATGCCACTTCCATCCGCAAA
GCTGTCTTAGCATCCATGGACAATGAAAATATGCATACCCCAGATATTGGAGGCCAGGGCACCACATCCC
AAGCCATCCAGGACATCATTCGTCATATCCGCATCATTAATGGACGGGCTGTGGAGGCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216960 representing NM_008323
Red=Cloning site Green=Tags(s)

MALKVAIAAGGAAKAMLKPTLLCRPWEVLAAHVAPRRSISSQQTIPPSAKYGGRHTVTMIPGDGIGPELM
LHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLD
LYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQE
SGRKKVTAVHKANIMKLGDGLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIV
NNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRK
AVLASMDNENMHTPDIGGQGTTSQAIQDIIRHIRIINGRAVEA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008323
ORF Size 1179 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008323.1, NP_032349.1
RefSeq Size 1335 bp
RefSeq ORF 1182 bp
Locus ID 15929
UniProt ID P70404
Cytogenetics X 37.41 cM
Summary Regulatory subunit which plays a role in the allosteric regulation of the enzyme catalyzing the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008323" in other vectors (4)
SKU Description Size Price
MC208738 Idh3g (untagged) - Mouse isocitrate dehydrogenase 3 (NAD+), gamma (Idh3g), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$503.00
MR216960 Idh3g (Myc-DDK-tagged) - Mouse isocitrate dehydrogenase 3 (NAD+), gamma (Idh3g), nuclear gene encoding mitochondrial protein 10 ug
$289.00 $457.00
MR216960L3 Lenti ORF clone of Idh3g (Myc-DDK-tagged) - Mouse isocitrate dehydrogenase 3 (NAD+), gamma (Idh3g), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR216960L4 Lenti ORF clone of Idh3g (mGFP-tagged) - Mouse isocitrate dehydrogenase 3 (NAD+), gamma (Idh3g), nuclear gene encoding mitochondrial protein 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.