Habp4 (NM_019986) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG216950
Habp4 (tGFP-tagged) - Mouse hyaluronic acid binding protein 4 (Habp4), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Habp4
Synonyms 4933413D03Rik; 4933428J01Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216950 representing NM_019986
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGGGGCCCTGGGGAGCCCTGTAGCCGCCGCGGGCGCCGCGATGCAGGAGACGTTCGGCTGCGTCG
TGGCCAACCGCTTCCATCAGCTGCTGGACGACGAGTCGGACCCGTTCGACATCCTGCGGGAGGCCGAGCA
CCGGCGCCAGCAGCAGCTGCAGCGCAAGCGTCGCGATGAGGCGGCGGCGGCGGCCAGCGGGGCCGGGCAC
CGTGGCGGCAGGAGCCCGGCTGTGGCCTCAGGACACAGGCCTGGCGCAGGCGGCCGCAGGGAGTCGCAGA
AGGAGCGCAAGAGCCTCGCGGCGTCCGGCGCACAGCAGCCAGACAGCCCTGGGGGCCCGCAGCCGCCAGG
CCAGAAGCGGACTCCTCGAAGAGGGGAGCAGCAAGGATGGAATGACAACAGGGGGACAGACGTGGTGCTT
GAAAGAGCAGAGCGAAGGTCCTACAGGGAATACCGGCCCTATGAGACTGAGAGACAGGCTGACTTGCCAG
TGGAGAAGTTTACCGATGAAAAACCAGTTGACAGGTTTGATCGAGACAGACCACTGAGAGGACGTGGAGG
CCCCAGAGGAGGCCTGAGGAGCAGAGGCCGAGGCGGTCCTGGGAACAGAGCTTTTGACTCCTTTGACCAA
AGAGGGAAACGAGACTTTGAGAGATACAGTAGCAATGATAAAACAAACAGAATGGAGGACAGCATGGGTG
GCTGTGGCATTCGCCCCTGGGGATCAGGTAAAGACACTAGTGATACAGAGCCACCTGCACCCATGGAAGA
GACCTCGATGATGGAGGAGTGCCAGGGCGCCCTGGACGAGGAGTCTGCAGCCAAAGTTCCTGAGTTGGAG
GTAGAAGAGGAAAACCAAGTCCAAGAGATGACCTTAGATGAGTGGAAAAACCTTCAAGAACAAACCAGAC
CAAAGCCTGAGTTCAATATTCGGAAGCCAGAGTCCACAGTTCCTTCCAAGGCAGTGGTGATTCACAAGTC
CAGATACAGAGACGATATGGTGAAGGAGGACTATGAGGATGAGTCTCACGTCTTCCGGAAAGCTGCCAAT
GACATCACATCCCAGCTGGAGATTAACTTTGGTAACCTCCCTCGCCCTGGTCGGGGAGCCAGAGGCAGCA
CCCGGGGAGGCCGAGGGAGAATGAGAAGAACAGAGAACTATGGCCCCCGTGCAGAAGTGGTGACCCAAGA
TGTTGCTCCCAACCCGGATGACCCTGAGGACTTCCCCGCCCTGGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216950 representing NM_019986
Red=Cloning site Green=Tags(s)

MKGALGSPVAAAGAAMQETFGCVVANRFHQLLDDESDPFDILREAEHRRQQQLQRKRRDEAAAAASGAGH
RGGRSPAVASGHRPGAGGRRESQKERKSLAASGAQQPDSPGGPQPPGQKRTPRRGEQQGWNDNRGTDVVL
ERAERRSYREYRPYETERQADLPVEKFTDEKPVDRFDRDRPLRGRGGPRGGLRSRGRGGPGNRAFDSFDQ
RGKRDFERYSSNDKTNRMEDSMGGCGIRPWGSGKDTSDTEPPAPMEETSMMEECQGALDEESAAKVPELE
VEEENQVQEMTLDEWKNLQEQTRPKPEFNIRKPESTVPSKAVVIHKSRYRDDMVKEDYEDESHVFRKAAN
DITSQLEINFGNLPRPGRGARGSTRGGRGRMRRTENYGPRAEVVTQDVAPNPDDPEDFPALA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019986
ORF Size 1236 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019986.3, NP_064370.2
RefSeq Size 2584 bp
RefSeq ORF 1239 bp
Locus ID 56541
UniProt ID Q9JKS5
Cytogenetics 13 33.26 cM
Summary RNA-binding protein that plays a role in the regulation of transcription, pre-mRNA splicing and mRNA translation. Negatively regulates DNA-binding activity of the transcription factor MEF2C in myocardial cells in response to mechanical stress. Plays a role in pre-mRNA splicing regulation. Binds (via C-terminus) to poly(U) RNA. Involved in mRNA translation regulation, probably at the initiation step. Seems to play a role in PML-nuclear bodies formation (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019986" in other vectors (4)
SKU Description Size Price
MC210074 Habp4 (untagged) - Mouse hyaluronic acid binding protein 4 (Habp4), (10ug) 10 ug
$503.00
MR216950 Habp4 (Myc-DDK-tagged) - Mouse hyaluronic acid binding protein 4 (Habp4) 10 ug
$289.00 $457.00
MR216950L3 Lenti ORF clone of Habp4 (Myc-DDK-tagged) - Mouse hyaluronic acid binding protein 4 (Habp4) 10 ug
$757.00
MR216950L4 Lenti ORF clone of Habp4 (mGFP-tagged) - Mouse hyaluronic acid binding protein 4 (Habp4) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.