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Tecr (NM_027179) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216948
Tecr (tGFP-tagged) - Mouse trans-23-enoyl-CoA reductase (Tecr) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
  $530.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Tecr or NM_027179"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tecr
Synonyms 2410016D23Rik; A230102P12Rik; AI173355; D17Ertd178e; Gpsn2; SC2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216948 representing NM_027179
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCACTACGAGGTGGAGATTCGGGATGCAAAGACGAGGGAGAAGCTGTGCTTCCTGGACAAGGTAG
AGCCTCAGGCCACCATTTCTGAAATCAAGACCCTTTTCACCAAGACACAGGGGAAGTCCCTGAAAGATGA
AGATGTCTTACAGAAGCTTCCTGTGGGCACCACAGCCACACTCTACTTCCGGGACCTCGGGGCCCAGATC
AGCTGGGTGACGGTCTTCCTGACGGAGTATGCCGGGCCCCTTTTCATCTACCTGCTCTTCTACTTCCGGG
TACCCTTCATTTATGGCCGCAAATACGACTTTACGTCCAGTCGGCATACGGTGGTGCACCTCGCCTGCAT
GTGCCACTCGTTCCACTACATCAAGCGCCTGCTGGAGACTCTCTTCGTGCACCGATTCTCTCACGGAACC
ATGCCTTTGCGAAACATCTTCAAAAACTGCACCTACTATTGGGGCTTTGCTGCATGGATGGCTTATTACA
TCAACCACCCTCTCTACACACCCCCTACCTATGGAGTTCAGCAGGTTAAGCTGGCACTGGCCGTTTTTGT
GATCTGCCAGCTTGGGAACTTCTCCATCCACATGGCTCTTCGGGACCTTCGGCCTGCTGGGTCGAAAACC
AGGAAGATCCCATACCCCACCAAGAACCCCTTCACCTGGCTGTTCCTGTTGGTGTCCTGTCCCAACTACA
CTTATGAGGTGGGCTCCTGGATTGGCTTTGCCATCTTGACTCAGTGTGTCCCAGTGGCCCTCTTCTCCCT
GGTGGGCTTCACCCAGATGACTATCTGGGCCAAGGGCAAACACCGCAGCTACCTGAAGGAGTTCCGCGAC
TACCCGCCCCTGCGCATGCCCATTATTCCCTTCCTGCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216948 representing NM_027179
Red=Cloning site Green=Tags(s)

MKHYEVEIRDAKTREKLCFLDKVEPQATISEIKTLFTKTQGKSLKDEDVLQKLPVGTTATLYFRDLGAQI
SWVTVFLTEYAGPLFIYLLFYFRVPFIYGRKYDFTSSRHTVVHLACMCHSFHYIKRLLETLFVHRFSHGT
MPLRNIFKNCTYYWGFAAWMAYYINHPLYTPPTYGVQQVKLALAVFVICQLGNFSIHMALRDLRPAGSKT
RKIPYPTKNPFTWLFLLVSCPNYTYEVGSWIGFAILTQCVPVALFSLVGFTQMTIWAKGKHRSYLKEFRD
YPPLRMPIIPFLL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027179
ORF Size 879 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027179.1, NP_081455.1
RefSeq Size 1145 bp
RefSeq ORF 882 bp
Locus ID 106529
UniProt ID Q9CY27
Cytogenetics 8 40.22 cM
Summary Involved in both the production of very long-chain fatty acids for sphingolipid synthesis and the degradation of the sphingosine moiety in sphingolipids through the sphingosine 1-phosphate metabolic pathway (By similarity). Catalyzes the last of the four reactions of the long-chain fatty acids elongation cycle (By similarity). This endoplasmic reticulum-bound enzymatic process, allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle (By similarity). This enzyme reduces the trans-2,3-enoyl-CoA fatty acid intermediate to an acyl-CoA that can be further elongated by entering a new cycle of elongation (By similarity). Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators (By similarity). Catalyzes the saturation step of the sphingosine 1-phosphate metabolic pathway, the conversion of trans-2-hexadecenoyl-CoA to palmitoyl-CoA (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027179" in other vectors (4)
SKU Description Size Price
MC212125 Tecr (untagged) - Mouse trans-2,3-enoyl-CoA reductase (Tecr), transcript variant 2, (10ug) 10 ug
$330.00
MR216948 Tecr (Myc-DDK-tagged) - Mouse trans-2,3-enoyl-CoA reductase (Tecr), transcript variant 2 10 ug
$330.00
MR216948L3 Lenti ORF clone of Tecr (Myc-DDK-tagged) - Mouse trans-2,3-enoyl-CoA reductase (Tecr), transcript variant 2 10 ug
$630.00
MR216948L4 Lenti ORF clone of Tecr (mGFP-tagged) - Mouse trans-2,3-enoyl-CoA reductase (Tecr), transcript variant 2 10 ug
$630.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.