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Slc14a1 (NM_028122) Mouse Tagged ORF Clone

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SKU
MG216943
Slc14a1 (tGFP-tagged) - Mouse solute carrier family 14 (urea transporter) member 1 (Slc14a1) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc14a1
Synonyms 2610507K20Rik; 3021401A05Rik; UT-B; Utb1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216943 representing NM_028122
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGATAGTCCCACCATGGTTAAAGTAGACCGGGGTGAAAACCAGATTTTATCATGCCGCGGGAGAA
GGTGTGGCTTCAAAGTACTTGGCTACGTCACGGGTGACATGAAGGAATTCGCCAACTGGCTGAAAGACAA
ACCCGTGGTGCTCCAGTTCATGGACTGGATACTTCGTGGCATATCCCAGGTGGTGTTTGTCAGCAACCCC
ATCAGTGGAATCCTGATTCTGGTGGGACTTCTGGTCCAGAACCCCTGGTGGGCTCTCTGTGGCTGTGTAG
GAACTGTGGTCTCCACTCTGACAGCCCTCTTGCTTAGCCAAGACAGATCGGCGATAGCAGCGGGGCTCCA
AGGTTACAATGCCACCCTGGTAGGCATCCTCATGGCTGTCTTCTCAAACAAGGGCGACTATTTCTGGTGG
CTGATATTCCCTGTATCTGCTATGTCTATGACTTGCCCGGTTTTCTCGAGCGCGTTGAGCTCCGTGCTCA
GCAAGTGGGACCTGCCCGTCTTCACTCTCCCTTTCAACATGGCGTTGTCGATGTACCTGTCAGCCACAGG
ACACTACAATACGTTTTTCCCAAGTAAACTCTTCACACCTGTCAGCTCCGTGCCCAACATCACGTGGTCT
GAGCTCAGCGCCCTGGAGCTATTGAAGTCTCTTCCGGTGGGAGTCGGTCAGATATATGGCTGTGACAACC
CGTGGACAGGCGGCATTTTCCTATGTGCTATCCTGCTCTCCTCCCCACTCATGTGCCTGCACGCTGCTAT
TGGATCGTTGCTGGGTGTCATCGCGGGACTCAGTCTTGCAGCTCCATTTGAAGACATCTACTTTGGGCTC
TGGGGTTTCAACAGCTCTCTGGCCTGCATTGCAATTGGAGGGATGTTCATGGCACTCACCTGGCAGACCC
ACCTCCTGGCTCTTGCCTGTGCCCTGTTCACTGCCTACTTCGGAGCCTGTATGGCACACCTGATGGCTGT
GGTTCACCTGCCAGCTTGTACCTGGTCCTTCTGTTTGGCCACACTACTCTTTCTCTTGCTGACCACGAAA
AATCCCAACATCTACAGGATGCCCCTCAGCAAAGTTACCTACTCTGAGGAGAACCGCATCTTCTACCTCC
AAAACAAGAAAAGGATGGTTGAAAGCCCCCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216943 representing NM_028122
Red=Cloning site Green=Tags(s)

MEDSPTMVKVDRGENQILSCRGRRCGFKVLGYVTGDMKEFANWLKDKPVVLQFMDWILRGISQVVFVSNP
ISGILILVGLLVQNPWWALCGCVGTVVSTLTALLLSQDRSAIAAGLQGYNATLVGILMAVFSNKGDYFWW
LIFPVSAMSMTCPVFSSALSSVLSKWDLPVFTLPFNMALSMYLSATGHYNTFFPSKLFTPVSSVPNITWS
ELSALELLKSLPVGVGQIYGCDNPWTGGIFLCAILLSSPLMCLHAAIGSLLGVIAGLSLAAPFEDIYFGL
WGFNSSLACIAIGGMFMALTWQTHLLALACALFTAYFGACMAHLMAVVHLPACTWSFCLATLLFLLLTTK
NPNIYRMPLSKVTYSEENRIFYLQNKKRMVESPL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028122
ORF Size 1152 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028122.4, NP_082398.1
RefSeq Size 3675 bp
RefSeq ORF 1155 bp
Locus ID 108052
UniProt ID Q8VHL0
Cytogenetics 18 E3
Summary Urea channel that facilitates transmembrane urea transport down a concentration gradient. A constriction of the transmembrane channel functions as selectivity filter through which urea is expected to pass in dehydrated form. The rate of urea conduction is increased by hypotonic stress. Plays an important role in the kidney medulla collecting ducts, where it allows rapid equilibration between the lumen of the collecting ducts and the interstitium, and thereby prevents water loss driven by the high concentration of urea in the urine. Facilitates urea transport across erythrocyte membranes. May also play a role in transmembrane water transport, possibly by indirect means.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028122" in other vectors (5)
SKU Description Size Price
MC212166 Slc14a1 (untagged) - Mouse solute carrier family 14 (urea transporter), member 1 (Slc14a1), transcript variant 2, (10ug) 10 ug
$503.00
MG205999 Slc14a1 (tGFP-tagged) - Mouse solute carrier family 14 (urea transporter), member 1 (Slc14a1) 10 ug
$657.00
MR216943 Slc14a1 (Myc-DDK-tagged) - Mouse solute carrier family 14 (urea transporter), member 1 (Slc14a1), transcript variant 2 10 ug
$289.00 $457.00
MR216943L3 Lenti ORF clone of Slc14a1 (Myc-DDK-tagged) - Mouse solute carrier family 14 (urea transporter), member 1 (Slc14a1), transcript variant 2 10 ug
$757.00
MR216943L4 Lenti ORF clone of Slc14a1 (mGFP-tagged) - Mouse solute carrier family 14 (urea transporter), member 1 (Slc14a1), transcript variant 2 10 ug
$757.00

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