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Als2cr12 (NM_175370) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216937
Als2cr12 (tGFP-tagged) - Mouse amyotrophic lateral sclerosis 2 (juvenile) chromosome region candidate 12 (human) (Als2cr12), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Als2cr12
Synonyms 4933405P16Rik; 4933425F06Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216937 representing NM_175370
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCCAATCCTTTCATCTACTGTACCTGCTGTGACTGCTGCAAATTGGGACAACCAAAGCTAATCA
AGACCCCTTATCCACTTCCAAAGCACCCCACGGGGAAGTTTAAGCCAGTTTTACCTCCACCAATTTCAAA
AGAACACAACTCACTCCTGTCCCAACCAGGAAAATCTACTGTTTCCCCAAGGGACAAGGTCCAGTCAGGA
AACACAGAGAGCAGCAAAGCACCTTCTGAAGTGATTCAAGTGTCACCTGGCTATACACTCATTCGAAATC
GAGAACAGATATCAGTCACCTTGGGAGATGAGATGTTCAACAGGAAAAAGCACTTGGAATCAGATGTCTT
GAGCAAAGTCAAATTTTCCAGGACTGATATCATTTCTGACCTTCAGGAGCAAATTGCTGAGTTGATGGCA
ATAATAGAACAGATGAACAGAGACCAGCAGTCTGCCCTGAAACTGCTCTCCAAAGATCTGGATCTCCGCT
GTTCCAACATGAAGCAGAAGTTTGAAACCGAGAGCAGGGAACTCAAAGAGACTCACAGAGAGGAGCTCGA
GAGGTTAGAGAACAACTACAAGGAGGCCCTGAAGGCAGAAAAGGCCTTGGCCGAAGAGAAACTAGATAAG
ATGAGCAAGGAATATAAGTATCTGAAGAGTATGTTCCATGTGTTTCAGGATAGCATTTACGAGGAAATGG
AAGACAAGTGGCTGAGACGAAAGGCCGAATGGGAGAAAGATGAGAAGATGGAGCGGGAAAAGATCCTGCT
ACAACAAAAATGTAGAATAATAAAAAAGTTTGAGTTACAGTCGGAAGAAAAAAAGAAGAAAATGAATGAG
TCCATCAGTGCTGTTTCTGATAACTTCGCTCGAGAGAAAGAGGAGCTCCTAAGACAACATGATGAAGACA
TCCTGCAAATACAGGAGCTGAGAAAATCCAAAGAGATCTTGGAGGCTGAGTTGCGGGCACAAGCTACTGT
TCTGGAGACCCTGAACACAAACCTTTTCCAATGCCAGAAAGAACTCGAGAGACAGAAAACTATTGCGGCA
AATCTAGAGAAATTGTTCCAGACAAAGCTTGCTGAAGCTGAAGAAAAGCATAAGTACAACATAAAGACCC
CGACAGAAGAAAATAACTGCCTCAGATGT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216937 representing NM_175370
Red=Cloning site Green=Tags(s)

MDPNPFIYCTCCDCCKLGQPKLIKTPYPLPKHPTGKFKPVLPPPISKEHNSLLSQPGKSTVSPRDKVQSG
NTESSKAPSEVIQVSPGYTLIRNREQISVTLGDEMFNRKKHLESDVLSKVKFSRTDIISDLQEQIAELMA
IIEQMNRDQQSALKLLSKDLDLRCSNMKQKFETESRELKETHREELERLENNYKEALKAEKALAEEKLDK
MSKEYKYLKSMFHVFQDSIYEEMEDKWLRRKAEWEKDEKMEREKILLQQKCRIIKKFELQSEEKKKKMNE
SISAVSDNFAREKEELLRQHDEDILQIQELRKSKEILEAELRAQATVLETLNTNLFQCQKELERQKTIAA
NLEKLFQTKLAEAEEKHKYNIKTPTEENNCLRC

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175370
ORF Size 1149 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175370.5, NP_780579.1
RefSeq Size 1579 bp
RefSeq ORF 1152 bp
Locus ID 108812
UniProt ID Q8BVM7
Cytogenetics 1 C1.3
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Documents
Resources
"NM_175370" in other vectors (4)
SKU Description Size Price
MC212182 Als2cr12 (untagged) - Mouse amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 12 (human) (Als2cr12), (10ug) 10 ug
$503.00
MR216937 Als2cr12 (Myc-DDK-tagged) - Mouse amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 12 (human) (Als2cr12) 10 ug
$289.00 $457.00
MR216937L3 Lenti ORF clone of Als2cr12 (Myc-DDK-tagged) - Mouse amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 12 (human) (Als2cr12) 10 ug
$757.00
MR216937L4 Lenti ORF clone of Als2cr12 (mGFP-tagged) - Mouse amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 12 (human) (Als2cr12) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.