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Haus8 (NM_029621) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216935
Haus8 (tGFP-tagged) - Mouse 4HAUS augmin-like complex subunit 8 (Haus8) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP tGFP DDK
  $703.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Haus8 or NM_029621"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Haus8
Synonyms 2410004L22Rik; Hice1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216935 representing NM_029621
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATTCCTCAGAGCGGGACGCAGGGAAGTCTGCAGCTGCTGGTGCTTGTGCTGTTCCCAAGACAA
AGGGAAGGAGAGTGCAAGGAAGAAGAGTGGTGGAGTCCCGGTATCTGCAATATGACAAGAAAGCAAAAAA
GGTTTCTGTAGCGGCCAAAGAAGAGAAGCCACCTGAAGGAAGGAAAGCCAGCACAGTGCCTAGGAGCAGA
GAAGAGAGCCAAGTGATGGGGACAGGCAACCTGCAGTCTACCATGCTAGAAGGGCATGGCATGAATCCAC
CAGACCTAGACCTCTCTGCCATCGACGACAAAATCTTGTCCAGGAAGGCTTCTTGGCCAGACAGGGAAAT
GACAGACAAAGCTAAGTCCACATCTTTTATCAGCTGTGACAAAAAAAGAATCCTTCGGAAGAAGAGACGG
GATCTGCAGGAGACCATGGACATGATGGAGTCACAGACACTGCTGATGACCCTGCTGTCTGTGAAGATGG
AGAACAACCTGGCTCTGCTGGAGGAGAGGGCAGAGAAAGACTTGGCAGCCATGTGCCATGAGAAAGAGAG
GCTGCAGAGGCAGGCACTGGAGCTTCGGCGCCAGCTGCTTCTCCGGCAGAAACATCAGGAGCTGGCCGCC
ACCCTTGATGCCCAGATTGAGGTGCTGAGTCCTCTTCAGCCTGTGTTAGAGCGCTTCAAAGAGGAATACA
TGACACTGGGCAGAGCCCTGGACACCACGCGACATGAGCTGCCCATGCAGGCTGTCCACATGGAAGGAAG
TGGGCAGGAGCTCCTAGATGATCTGGAGCCAGCCCTGAGGATCACCCTGCAGCTCCTGGGTGATCTGAGC
ATCTGCTCCCCATATGCCACTGCACAGGTGCAGGGTGCCAGCGCACAGGAGCCTGGAGCCAGCACACAGC
TGAGTTGTCTGCTGAAGGAACTGAAGGGCTTGGTCACTGAGAAAGACCTGGAGCTCCGCAGACTTGTCAG
CCAGGTGGTGGAACTTTCCTCCCAGGCCAGTAAAGAAGCAGCTTTGATGAACCAGGAAGTCTGGGAAGAG
GCAGAGGGTGCCCTCACCAGCAGCCAGTGGTACTTCAGTCCAGATGCCTGCAGGGATGATAGTCCCTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216935 representing NM_029621
Red=Cloning site Green=Tags(s)

MADSSERDAGKSAAAGACAVPKTKGRRVQGRRVVESRYLQYDKKAKKVSVAAKEEKPPEGRKASTVPRSR
EESQVMGTGNLQSTMLEGHGMNPPDLDLSAIDDKILSRKASWPDREMTDKAKSTSFISCDKKRILRKKRR
DLQETMDMMESQTLLMTLLSVKMENNLALLEERAEKDLAAMCHEKERLQRQALELRRQLLLRQKHQELAA
TLDAQIEVLSPLQPVLERFKEEYMTLGRALDTTRHELPMQAVHMEGSGQELLDDLEPALRITLQLLGDLS
ICSPYATAQVQGASAQEPGASTQLSCLLKELKGLVTEKDLELRRLVSQVVELSSQASKEAALMNQEVWEE
AEGALTSSQWYFSPDACRDDSPS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029621
ORF Size 1119 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029621.3, NP_083897.2
RefSeq Size 1683 bp
RefSeq ORF 1119 bp
Locus ID 76478
UniProt ID Q99L00
Cytogenetics 8 B3.3
Summary Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_029621" in other vectors (5)
SKU Description Size Price
MC200334 Haus8 (untagged) - Mouse 4HAUS augmin-like complex, subunit 8 (Haus8), transcript variant 2, (10ug) 10 ug
$457.00
MG205745 Haus8 (tGFP-tagged) - Mouse RIKEN cDNA 2410004L22 gene (2410004L22Rik) 10 ug
$657.00
MR216935 Haus8 (Myc-DDK-tagged) - Mouse 4HAUS augmin-like complex, subunit 8 (Haus8), transcript variant 2 10 ug
$503.00
MR216935L3 Lenti ORF clone of Haus8 (Myc-DDK-tagged) - Mouse 4HAUS augmin-like complex, subunit 8 (Haus8), transcript variant 2 10 ug
$803.00
MR216935L4 Lenti ORF clone of Haus8 (mGFP-tagged) - Mouse 4HAUS augmin-like complex, subunit 8 (Haus8), transcript variant 2 10 ug
$803.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.