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Popdc2 (NM_022318) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216917
Popdc2 (tGFP-tagged) - Mouse popeye domain containing 2 (Popdc2) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Popdc2 or NM_022318"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Popdc2
Synonyms AV006127; P; Pop2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216917 representing NM_022318
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCCAATGGCAGCTCGGTGGCTCAGCTCCTCTGGCAGCCGCCCGTGTGCAGGAGCTGGAAACCGG
ATGTAGAAGGAGCTGTCTATCACCTGGCCAACTGTTTCTTGCTTATGGGCTTTATGGCAGGCAGTGGAGT
GTATGGATGCTTCTATCTTTTCGGCATCCTGGGCCCAGGCTACCTCTGCTGCGTGCTGTGGGGCTGGTTT
GATGCTTGTGGACTAGACATCGTCCTTTGGAACGTCCTCCTGACAGTGGCTTGCCTGCTTCAGCTGGCAC
AGCTGGTCTATCGTGTCCGGGTGAATACCCTCCCGGAGGAATTCAATCTCCTCTACAGGACACTGTGCCT
GCCCCTGCAGGTGCCCCTGCAGGTCTACAAGGAGATTGTCCACTGCTGCCATGAGCAGGTCTTGACGCTG
GCCACAGAGCAGACCTACGCTGTGGAGGGGGAGACCCCCATCAATCGCCTGTCCCTGCTCCTCTCAGGCC
GGGTTCGAGTGAGCCAAGACGGGCAGTTTCTGCACTACATCTTTCCGTATCAGTTCATGGACTCTCCTGA
ATGGGAATCACTGCATCCTTCTGAAGAGGGGACCTTCCAGGTGACGCTGACTGCGGAGACCGAATGTAGC
TACATTTCCTGGCCCCGGAAAAATCTTTACCTTCTTCTGAACAGAGAGCGATACATCTCCCGCCTCTTCT
CAGCCCTGCTGGGCTATGACATCTCGGAGAAGCTCTATACCCTCAATGACAAGCTGTTTGCCAAGTTTGG
GTTACGCTTCGACATCCGCCTTCCCAGCCTCTACCACGTTCTGAGTCCCTCTGCCTCAGACGGGGAACCA
GAGTCTGAGAAAGATGATGAAGAAGCCCTTGAGGCAGCTGTGTCCCCTGCTCAGGCCAGGCCCATCTGCA
TCGTGCCAACACCCCCTTGTTCCGCGCCTCCAGCAACCACCAACTTTCCCGTGCCTCTGCCCCGGGCCAG
GATGCCCAGGATGCCCAGGCCGGACAGCGGCAACCTGGCCTCTAGACGTCCTCTCCAGAACTCTTCTCAA
GTTATGTCCAGATCCCAGGCCCCTCTGGCTCCAATTCACACTCCTGAGCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216917 representing NM_022318
Red=Cloning site Green=Tags(s)

MSANGSSVAQLLWQPPVCRSWKPDVEGAVYHLANCFLLMGFMAGSGVYGCFYLFGILGPGYLCCVLWGWF
DACGLDIVLWNVLLTVACLLQLAQLVYRVRVNTLPEEFNLLYRTLCLPLQVPLQVYKEIVHCCHEQVLTL
ATEQTYAVEGETPINRLSLLLSGRVRVSQDGQFLHYIFPYQFMDSPEWESLHPSEEGTFQVTLTAETECS
YISWPRKNLYLLLNRERYISRLFSALLGYDISEKLYTLNDKLFAKFGLRFDIRLPSLYHVLSPSASDGEP
ESEKDDEEALEAAVSPAQARPICIVPTPPCSAPPATTNFPVPLPRARMPRMPRPDSGNLASRRPLQNSSQ
VMSRSQAPLAPIHTPEL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022318
ORF Size 1101 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022318.3
RefSeq Size 2027 bp
RefSeq ORF 1104 bp
Locus ID 64082
UniProt ID Q9ES82
Cytogenetics 16 B3
Summary This gene encodes a member of the Popeye domain containing family of membrane proteins. Proteins of this family contain three helical transmembrane domains and a conserved intracellular Popeye domain. In the adult mouse, this gene is expressed at high levels in cardiac myocytes, and mice deficient for this gene develop stress-induced cardiac pacemaker dysfunction. The protein binds to a two-pore domain potassium channel and recruits it to the plasma membrane. Cyclic adenosine monophosphate negatively regulates this interaction through the Popeye domain. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2015
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Documents
Resources
"NM_022318" in other vectors (3)
SKU Description Size Price
MR216917 Popdc2 (Myc-DDK-tagged) - Mouse popeye domain containing 2 (Popdc2), transcript variant 2 10 ug
$289.00 $457.00
MR216917L3 Lenti ORF clone of Popdc2 (Myc-DDK-tagged) - Mouse popeye domain containing 2 (Popdc2), transcript variant 2 10 ug
$757.00
MR216917L4 Lenti ORF clone of Popdc2 (mGFP-tagged) - Mouse popeye domain containing 2 (Popdc2), transcript variant 2 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.