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Rb1cc1 (NM_009826) Mouse Tagged ORF Clone

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SKU
MG216914
Rb1cc1 (tGFP-tagged) - Mouse RB1-inducible coiled-coil 1 (Rb1cc1), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,559.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rb1cc1
Synonyms 2900055E04Rik; 5930404L04Rik; Cc1; FIP200; LaXp180
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216914 representing NM_009826
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTTATATGTGTTTCTGGTTAACACCGGAACCACGCTGACATTTGACACTGAGCTAACTGTGCAAA
CTGTGGCTGATCTTAAGCATGCCATTCAAAGCAAATACAAGATTGCTATTCAGCACCAGGTTCTGGTGGT
CAATGGAGGAGAATGCATGGCTGCAGATCGAAGAGTGTGTACTTACAGCGCTGGGACGGACACAAATCCA
ATTTTTCTTTTTAATAAAGAAATGATCTTATGTGACCGTGCACCTGCTATTCCTAAAGCTACCTTTTCAA
CAGAAAATGACATGGAAATAAAAGTTGAAGAGTCTCTTATGATGCCTGCAGTTTTCCACACTGTTGCTTC
AAGGACACAGCTTGCAGTGGAAATGTATGACGTTGCCAAGAAGCTCTGCTCTTTCTGTGAAGGGCTTGTC
CATGATGAACATCTTCAGCACCAAGGCTGGGCTGCAATCATGGCCAATCTGGAGGACTGTTCAAATTCAT
ACCAAAAACTTCTTTTCAAGTTTGAAAGTATTTATTCTGATTATCTTCAATCCATAGAAGACATCAAGTT
AAAACTTACTCATTTAGGAACTGCTGTTTCAGTAATGGCCAAGATTCCACTATTGGAGTGCCTAACCAGA
CATAGTTACAGGGAATGTTTGGGAAGACCGGATTCTTTGAATGAACATGAAGGCTCAGAGAAAGCTGAGA
TGAAAAGATCTACTGAATTGGTGCTCTCTCCTGATATGCCTAGAACATCGAACACATCCTTGGTAACCTC
ATTTCACAAGTCAATGGAGCATGTAGCTCCAGATCCCACCGGTACTGAACGTGGCAAAGAACTTAGGGAA
TCTTGTCAAAGTACTGTCCAGCAAGAAGAAGCTTCAGTGGATGCTAAAGACAGTGATCTGCCTTTTTTTA
ATGTTTCTTTGTTAGACTGGATAAATGTTCAAGATAGACCCAATGATGTGGAATCTCTGGTCAGGAAGTG
CTTTGATTCTATGAGCAGGCTTGACCCAAAGATTATTCAACCATTTATGTTAGAATGCCATCAAACTATT
GCCAAACTTGATAATCAGAATATGAAAGCCATTAAAGGGCTTGAAGATCGGCTGTATGCCTTGGACCAGA
TGATTGCTAGCTGTAGCCGGCTGGTAAATGAACAGAAAGAGCTTGCTCAGGGATTTTTAGCTAATCAGAT
GAGAGCTGAAAACTTGAAGGATGCATCTGTGTTACCTGATCTGTGTCTGAGTCATGCAAATCAACTAATG
ATTATGTTGCAAAACCACAGAAAACTGTTGGATATTAAACAGAAGTGCACCACTGCCAAACAAGAGCTAG
CAAACAATCTCCACGTCAGACTGAAGTGGTGTTGTTTTGTGATGCTTCATGCTGATCAAGATGGAGAAAA
ACTGCAGGCACTGCTCCGCCTTGTAATAGAGCTGTTAGAAAGAGTCAGAATTGTTGAGGCTCTTAGTACA
GTTCCTCAGATGTATTGCCTAGCTGTTGTTGAGGTTGTAAGAAGAAAAATGTTCATTAAACACTACAGAG
AGTGGGCTGGTGCTTTAGTCAAAGACGGAAAACAACTATATGAAGCTGAAAAGTCAAAAAGGGAATCCTT
TGGGAAATTATTTAGGAAGTCCTTTTTAAGAAATCGTCTGTTTAAAGGACTGGACTCCTGGCCTTCCTCA
TTTTGTACTCAGAAGCCTCGAAAATTTGACTGTGAACTTCCAGATATATCGTTAAAAGATTTACAGTTTC
TTCAATCATTTTGTCCTTCAGAAGTGCAGCCATTCCTCAGGGTCCCCTTACTTTGTGACTTTGAACCTCT
ACACCAGCATGTACTTGCCCTACATAATTTGGTAAAAGCAGCACAAAGTTTGGATGAAATGTCACAGACT
ATTACAGATCTCCTAAATGAACAAAAGGTATCCACAAGTCAGGCATCCCCACAGTCAGCTGCTTCTCCAA
GAATAGAAAGTACAACAGGCATTACAACCACTACCTCACCAAAAACTCCTCCTCCACTAACTGTTCAGGA
CACCTTATGTCCGGCAGTGTGTCCCTTAGAAGAATTATCTCCAGATAGTATCGATGCTCATACATTTGAT
TTTGAAACCATCTCCCATCCAAACACAGAACAACCTGTTCACCAAGCTTCTATAGACTTGGATTCATTAG
CAGAAAGCCCTGAGTCTGACTTTATGTCTGCTGTGAATGAGTTTGTGATAGAAGAAAATTTATCGTCTCC
AAACCCTATAAGTGATCCACAAAGTCCAGAAATGATGGTGGAGTCACTTTACTCTTCAGTCATCAATGCA
ATAGATAGTAGGCGTATGCAAGACACAAGTACACGTGGAAACGAGGGCTTTGGGGATCGGGCTGCTCTAC
ATGTCCAGCTGGAGAAATGCAGAGCTGCTGCACAAGACTCTCACAGCAGTATACAAACCATCAAGGACGA
TCTGTGCCATTTCAGAACATTTGTACAAAAAGAACAGTGTGACTTAGCAAATTATTTAAAATGTACAGCT
GTAGAAATAAGAAATATTATTGAAAAAGTAAAATGTTCTCTAGAAATAACACTAAAGGAAAAGCATCAGC
AAGAACTCCAATCTTTAAAAATTGAGTATGAATGTAAACTTGATGCTCTAGTAAAAGACAGTGAAGAAAA
TGTAAATAAAATTTTAAAATTGAAAGAAAATTTAGTATCCCTTGAAGAGGCTTTACAAAATAAAGACAAT
GAATTCACTTCGATTAAACATGAAAAGGATGCTATTGTCTGTGTGCAGCAAGAAAAGGATCAGAAGTTGT
TAGAGATGGAAAAGATAATGCATACTCAACATTGTGAAATTAAAGAACTGAAGCAGTCACGAGAGATGGC
ATTAGAAGACCTGAAAAAGCTGCATGATGAAAAAATCGAGTCATTGAGAGCTGAATTTCAGTGCTTAGAA
CAAAATCACCTGAAGGAATTAGAGGACACACTGCACATCAGGCACACACAGGAGTTTGAGAAAGTTATGA
CAGACCACAATATGTCTTTGGAGAAATTAAAAAAAGAAAATCAGCAAAGAATTGACCAGATGCTAGAATC
TCATGCCTCAACTATTCAGGAAAAAGAGCAACAGCTGCAGGAGTTGAAACTCAAAGTTTCTGACTTGTCA
GACATGAGATGTAAGTTAGAGGTTGAACTTGCACTAAAGGAAGCAGAAACAGATGAGATAAAGATCTTGT
TGGAAGAGAGCAGAACACAGCAGAAGGAAATGCTGAAGTCTTTACTTGAACAAGAGACCGAAAACTTAAG
AACAGAAATAAGTAAACTAAACCAAAAAATTCATGATAATAATGAGAGTTACCAGGTGGGTTTGTCAGAG
TTAAGAGCTTTAATGACAATTGAAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAAGAAGAAT
CTAATATACTTAAGGCTGAATTAGACAATGTTACATCTTTGCATCGCCAAGCATTTGAAATAGAAAAAAA
ACTGAAAGAACAAATAGTTGAATTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTTGAAAAACAGAAA
GATGAAAAAATAACCCAACAAGAAGAGAAGTATGAAGCACGTATCCAGAACCTTGAGAAAGACAAGGAGA
GACTGGTCAAGAACCACGAGCAAGACAAAGAACACTTAATTCAGGAGCTTAATTTTGAAAAAAACAAAGC
TGTTCAAACTGCACTAGATGAATTTAAGGTGGAGAGAGAACTTGTTGAGAAAGAGTTATTAGAAAAAGTT
AAACATCTTGAGAATCAAATAGCCAAAACTCCTGCCTTTGAGTCAGCCAGAGAAGATTCTTCAAGCTTAG
TTGCGGAACTTCAAGAGAAACTTCAAGAAGAAAAAGCTAAGTTTCTGGAACAACTTGAAGAACAAGAGAA
AAGAAAGAATGAGGAAATGCAAAATGTCAGAACCTCTTTGATTGCTGAGCAGCAGACCAACTTTAACACA
GTCTTAACAAGAGAGAAAATGAGGAAAGAAAACATAATAAATGATCTTAGTGATAAGCTAAAAAGTACAA
TGCAGCAGCAAGAGCGGGATAAAGATTTGATAGAGTCGCTCTCTGAGGACCGAGCTCGTTTGCTTGAAGA
GAAGAAGCAGCTTGAAGAGGAAGTGAGTAAACTCCGCACTAGCAGTTTTCTTTCCTCAGCACCTGTGGCT
GCAGCCCCAGAGCTCTATGGTGCGTGTGCACCTGAGCTCCCAGGGGAGCCAGAGAGATCAGTCATGGAGA
CGGCAGATGAAGGAAGACTGGATTCCGCAATGGAGACAAGCATGATGTCTGTCCAAGAAAACATGTTATC
TGAAGAGAAGCAGAGGATCATGCTCCTAGAACGGACATTGCAGTTGAAAGAAGAAGAAAACAAGCGGTTA
AATCAAAGACTGATGTCTCAGAGTTTGTCCTCAGTCTCTTCAAGGCATTCTGAAAAAATAGCCATTAGAG
ATTTTCAGGTGGGAGATTTGGTTCTCATCATCCTAGATGAGCGGCACGACAATTATGTATTGTTTACTGT
TAGTCCTACTTTATATTTTCTGCACTCAGAGTCTCTTCCTGCCCTGGATCTCAAACCAGGTGAGGGAGCT
TCAGGTGCATCTAGAAGACCCTGGGTCCTTGGGAAAGTAATGGAAAAGGAATACTGTCAAGCCAAAAAGG
CACAAAACAGATTTAAAGTTCCTTTGGGGACAAAGTTTTACAGAGTGAAAGCTGTGTCATGGAATAAGAA
AGTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216914 representing NM_009826
Red=Cloning site Green=Tags(s)

MKLYVFLVNTGTTLTFDTELTVQTVADLKHAIQSKYKIAIQHQVLVVNGGECMAADRRVCTYSAGTDTNP
IFLFNKEMILCDRAPAIPKATFSTENDMEIKVEESLMMPAVFHTVASRTQLAVEMYDVAKKLCSFCEGLV
HDEHLQHQGWAAIMANLEDCSNSYQKLLFKFESIYSDYLQSIEDIKLKLTHLGTAVSVMAKIPLLECLTR
HSYRECLGRPDSLNEHEGSEKAEMKRSTELVLSPDMPRTSNTSLVTSFHKSMEHVAPDPTGTERGKELRE
SCQSTVQQEEASVDAKDSDLPFFNVSLLDWINVQDRPNDVESLVRKCFDSMSRLDPKIIQPFMLECHQTI
AKLDNQNMKAIKGLEDRLYALDQMIASCSRLVNEQKELAQGFLANQMRAENLKDASVLPDLCLSHANQLM
IMLQNHRKLLDIKQKCTTAKQELANNLHVRLKWCCFVMLHADQDGEKLQALLRLVIELLERVRIVEALST
VPQMYCLAVVEVVRRKMFIKHYREWAGALVKDGKQLYEAEKSKRESFGKLFRKSFLRNRLFKGLDSWPSS
FCTQKPRKFDCELPDISLKDLQFLQSFCPSEVQPFLRVPLLCDFEPLHQHVLALHNLVKAAQSLDEMSQT
ITDLLNEQKVSTSQASPQSAASPRIESTTGITTTTSPKTPPPLTVQDTLCPAVCPLEELSPDSIDAHTFD
FETISHPNTEQPVHQASIDLDSLAESPESDFMSAVNEFVIEENLSSPNPISDPQSPEMMVESLYSSVINA
IDSRRMQDTSTRGNEGFGDRAALHVQLEKCRAAAQDSHSSIQTIKDDLCHFRTFVQKEQCDLANYLKCTA
VEIRNIIEKVKCSLEITLKEKHQQELQSLKIEYECKLDALVKDSEENVNKILKLKENLVSLEEALQNKDN
EFTSIKHEKDAIVCVQQEKDQKLLEMEKIMHTQHCEIKELKQSREMALEDLKKLHDEKIESLRAEFQCLE
QNHLKELEDTLHIRHTQEFEKVMTDHNMSLEKLKKENQQRIDQMLESHASTIQEKEQQLQELKLKVSDLS
DMRCKLEVELALKEAETDEIKILLEESRTQQKEMLKSLLEQETENLRTEISKLNQKIHDNNESYQVGLSE
LRALMTIEKDQCISELISRHEEESNILKAELDNVTSLHRQAFEIEKKLKEQIVELQTRLNSELSALEKQK
DEKITQQEEKYEARIQNLEKDKERLVKNHEQDKEHLIQELNFEKNKAVQTALDEFKVERELVEKELLEKV
KHLENQIAKTPAFESAREDSSSLVAELQEKLQEEKAKFLEQLEEQEKRKNEEMQNVRTSLIAEQQTNFNT
VLTREKMRKENIINDLSDKLKSTMQQQERDKDLIESLSEDRARLLEEKKQLEEEVSKLRTSSFLSSAPVA
AAPELYGACAPELPGEPERSVMETADEGRLDSAMETSMMSVQENMLSEEKQRIMLLERTLQLKEEENKRL
NQRLMSQSLSSVSSRHSEKIAIRDFQVGDLVLIILDERHDNYVLFTVSPTLYFLHSESLPALDLKPGEGA
SGASRRPWVLGKVMEKEYCQAKKAQNRFKVPLGTKFYRVKAVSWNKKV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009826
ORF Size 4764 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009826.4, NP_033956.2
RefSeq Size 7046 bp
RefSeq ORF 4767 bp
Locus ID 12421
UniProt ID Q9ESK9
Cytogenetics 1 A1
Summary Involved in autophagy (PubMed:23262492, PubMed:19258318). Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1 (PubMed:23392225, PubMed:23285000, PubMed:19258318). Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (PubMed:21525242). Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (PubMed:21807966). Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways (By similarity). Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (PubMed:21795712). Functions as a DNA-binding transcription factor (PubMed:12095676). Is a potent regulator of the RB1 pathway through induction of RB1 expression (PubMed:15968549). Plays a crucial role in muscular differentiation (PubMed:15968549). Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (PubMed:19940130, PubMed:21088496).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009826" in other vectors (4)
SKU Description Size Price
MC224720 Rb1cc1 (untagged) - Mouse RB1-inducible coiled-coil 1 (Rb1cc1), (10ug) 10 ug
$1,359.00
MR216914 Rb1cc1 (Myc-DDK-tagged) - Mouse RB1-inducible coiled-coil 1 (Rb1cc1) 10 ug
$589.00 $1,359.00
MR216914L3 Lenti ORF clone of Rb1cc1 (Myc-DDK-tagged) - Mouse RB1-inducible coiled-coil 1 (Rb1cc1) 10 ug
$1,659.00
MR216914L4 Lenti ORF clone of Rb1cc1 (mGFP-tagged) - Mouse RB1-inducible coiled-coil 1 (Rb1cc1) 10 ug
$1,659.00

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