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Car1 (NM_001083957) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216910
Car1 (tGFP-tagged) - Mouse carbonic anhydrase 1 (Car1) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Car1
Synonyms AW555628; Ca1; Car-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216910 representing NM_001083957
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAGTGCAGACTGGGGATATGGAAGCGAAAATGGTCCTGACCAATGGAGCAAGCTGTATCCCATTG
CCAATGGAAACAACCAGTCTCCTATTGATATTAAAACCAGTGAAGCCAATCATGACTCCTCTCTGAAACC
ACTCAGCATCTCCTATAATCCTGCAACTGCCAAAGAAATTGTTAACGTGGGACATTCTTTCCATGTAATT
TTTGATGACAGTAGCAACCAATCTGTTCTGAAAGGTGGCCCTCTTGCTGATAGCTATCGGCTCACTCAGT
TCCATTTTCACTGGGGCAACTCAAACGACCATGGCTCTGAGCACACCGTGGATGGAACTAGATATTCTGG
AGAGCTTCACTTAGTTCACTGGAATTCTGCAAAGTACTCCAGTGCTTCTGAAGCCATCTCCAAGGCTGAT
GGCCTGGCAATCCTTGGCGTTTTGATGAAGGTTGGTCCAGCCAACCCAAGCCTGCAGAAAGTACTTGATG
CTCTAAACTCAGTTAAAACTAAGGGAAAACGAGCCCCATTCACAAATTTTGACCCATCCAGTCTGCTTCC
TTCATCTCTGGATTACTGGACCTACTTTGGCTCTCTGACTCACCCTCCTCTTCATGAAAGTGTGACCTGG
GTGATCTGCAAGGATAGCATCAGTCTAAGCCCAGAGCAGCTGGCCCAGCTCCGTGGTCTTCTGTCAAGTG
CAGAGGGAGAGCCTGCAGTTCCAGTTCTGAGCAACCACCGTCCACCCCAACCCCTGAAGGGCAGAACAGT
CAGAGCCTCATTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216910 representing NM_001083957
Red=Cloning site Green=Tags(s)

MASADWGYGSENGPDQWSKLYPIANGNNQSPIDIKTSEANHDSSLKPLSISYNPATAKEIVNVGHSFHVI
FDDSSNQSVLKGGPLADSYRLTQFHFHWGNSNDHGSEHTVDGTRYSGELHLVHWNSAKYSSASEAISKAD
GLAILGVLMKVGPANPSLQKVLDALNSVKTKGKRAPFTNFDPSSLLPSSLDYWTYFGSLTHPPLHESVTW
VICKDSISLSPEQLAQLRGLLSSAEGEPAVPVLSNHRPPQPLKGRTVRASF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001083957
ORF Size 783 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001083957.1, NP_001077426.1
RefSeq Size 1184 bp
RefSeq ORF 786 bp
Locus ID 12346
UniProt ID P13634
Cytogenetics 3 3.18 cM
Summary Reversible hydration of carbon dioxide.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001083957" in other vectors (3)
SKU Description Size Price
MR216910 Car1 (Myc-DDK-tagged) - Mouse carbonic anhydrase 1 (Car1), transcript variant 2 10 ug
$289.00 $300.00
MR216910L3 Lenti ORF clone of Car1 (Myc-DDK-tagged) - Mouse carbonic anhydrase 1 (Car1), transcript variant 2 10 ug
$600.00
MR216910L4 Lenti ORF clone of Car1 (mGFP-tagged) - Mouse carbonic anhydrase 1 (Car1), transcript variant 2 10 ug
$600.00

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