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Diexf (NM_145415) Mouse Tagged ORF Clone

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SKU
MG216881
Diexf (tGFP-tagged) - Mouse expressed sequence AA408296 (AA408296), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $1,255.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Diexf
Synonyms AA408296; AW045375; Def
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216881 representing NM_145415
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAAACGCCGGAACCGGGGCCGCAGCCAGATGCTCAGCACCATGACCAAGAAGCAGAAGAAGCACC
TCCGCGACTTCGGCGAGGAGCACCCCTTCTACGACAGGGTTTCCAAGAAGGAAGCCAAGCCGCAGATTTG
TCAGCTGCCAGAGAGTTCAGATTCTTCACATTCTGAAAGTGAATCAGAGAGTGAACAAGAGCACGTTTCA
GGGTACCACAGACTGCTTGCTACATTAAAGAATGTTTCTGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGG
AGGAGGAGGAGGAGGAGGAAGAGGAAGAGGAAGATGACAGTGCTGTAGGTGATGCAGAAATGAATGAGGA
AGCTGGCAGTGAGGATGGCAGTGTGGGAGAAGCTGCTGTCTCGGAGGCTGCTGAGGAGGCTGCTGAGACA
CAGGAACATATGTCTCTAGCTGACAACTCAAAAGAAAAAGATGGTGAAGAGCCACCTGGTGTATCACAGA
AATCCTCAGAGGAGTTCACAGATGTGAAACACGAGTCACTGTTCAGCCTGGAAACTAATTTTCTAGAAGA
GGATAGCGGGGGCAGTTGTTCCCAGAGGCCATCCCAAGATCCGTTTCAACAACATGTCAACAAAGAACTG
AAAGAGAAGGAAATTCAAGCTGCTGCTTCCAGCCCTCCAGCTACCCAGCAACTGAAATGGCCAGTCCTGG
GACATCTTGTCTTTTCATCAAAGTTCCAGAAGACGGAAACATTTAAACCTCCAAAGGATATTGACCTAAA
GTTACTTCATCTTCAGAAGCCTCTAGAATCCACCTGGGCAAAAACCAATAGCCAATTTCTATCTGGACCT
CAAAAATCAAATAGCTCCTTTACCCCGCTCCAGAAAGAGCTGTTCTTAATTATGAATTCTTACCGGGACC
TGTTCTACCCTGAGAGGACTGCCCTGAAGAATGGAGAAGAAGTCCGCCATGTATACTGCCTGCATGCCAT
AAACCATGTCCTCAAAGCCAATGCTCAGGTGCTGGCTAACAACAGTAGGCGCCGGAGCCAGAAACTTGGG
GTGGGTGAAGATGATGACTTCAGGGACCAAGGGCTCACCAGGCCTAAGGTACTTATAGTGGTGCCCTTCC
GGGAAGCTGCCCTTCGGGTGGTCCAGCTCTTCATCAGCCTCCTTGAAGGGGACAGCAAGAAGAAAATCAT
TGTAAGCAATAAAAAGCGGTTTCAGGGGGAGTATGGATCTGATCCTGAGGAGAGACCTCCCAACCTGAAG
AGGCCTGAGGATTATGAAGCTGTGTTCGTGGGCAATATTGATGACCACTTCAGGATTGGAGTGGCAATAC
TTCAGAGGAGCATCCGTCTCTACGCCCCGTTTTACTCCTCCGACATCCTCATTGCCTCCCCCCTGGGCTT
GAGGACCATCATTGGTGGTGAAGGAGAGAAGAAGAGAGATTTTGACTTCCTGTCTTCCGTTGAGCTGCTC
ATCATTGACCAGGCTGACATTTACCTGATGCAGAACTGGGAACATGTCTTGCATTTGATGAACCACATGA
ACCTGCTGCCCTTGGACTCACACGGGGTGGACTTCTCGAGAGTCCGAATGTGGAGCCTCAATAACTGGTC
CAAGTACTACCGACAGACACTGCTCTTTGGTGCCCTGCAGGATGCCCAGATCAACTCTGTGTTCAACAAG
CACTGCATCAATGCTCAAGGCCAGGTGGCTGTGAGGAATGTCCCCATGACGGGCTCCATCAGTCATGTGT
TGGTGCAGCTCCCTCACGTCTTCCAGAGAATGGAAGCACAAGACCTATCTTCAGTGATCGATGCCAGGTT
TCACTTTTTCATAAACAAGATTTTGCCTCAGTACCGGGATGCTGTCATGTCTCACACACTCATCTATGTC
CCCTCCTACTTTGACTTTGTGCGTCTCCGAAATTACTTCAAGAAAGAAGAGCTGAACTTCACACACATCT
GCGAGTATACGCAGAAGTCTGGGATCTCCAGGGCTCGACACTTCTTTCTGCAAGGAGAGAAGCAGTTTCT
GCTCCTCACAGAACGCTTCCATTTCTACAAAAGGTACACAATAAAAGGCATCAGGAACCTGATATTCTAT
GAACTGCCAACGTACCCTCACTTCTACAGTGAGGTCTGTAACATGCTGAGAGCCACCAGCAGAGGAGAGG
AGGCCACGTGGACGTGCACCGTGCTGTATTCCAAGTATGATGCCCAGAGGCTGGCGGCTGTGGTTGGTGT
GGAGCGGGCGGCACAGATGCTGCAGTCTCCAAAGAATGTCCACCTCTTCGTCACCGGAGAAAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216881 representing NM_145415
Red=Cloning site Green=Tags(s)

MGKRRNRGRSQMLSTMTKKQKKHLRDFGEEHPFYDRVSKKEAKPQICQLPESSDSSHSESESESEQEHVS
GYHRLLATLKNVSEEEEEEEEEEEEEEEEEEEEEDDSAVGDAEMNEEAGSEDGSVGEAAVSEAAEEAAET
QEHMSLADNSKEKDGEEPPGVSQKSSEEFTDVKHESLFSLETNFLEEDSGGSCSQRPSQDPFQQHVNKEL
KEKEIQAAASSPPATQQLKWPVLGHLVFSSKFQKTETFKPPKDIDLKLLHLQKPLESTWAKTNSQFLSGP
QKSNSSFTPLQKELFLIMNSYRDLFYPERTALKNGEEVRHVYCLHAINHVLKANAQVLANNSRRRSQKLG
VGEDDDFRDQGLTRPKVLIVVPFREAALRVVQLFISLLEGDSKKKIIVSNKKRFQGEYGSDPEERPPNLK
RPEDYEAVFVGNIDDHFRIGVAILQRSIRLYAPFYSSDILIASPLGLRTIIGGEGEKKRDFDFLSSVELL
IIDQADIYLMQNWEHVLHLMNHMNLLPLDSHGVDFSRVRMWSLNNWSKYYRQTLLFGALQDAQINSVFNK
HCINAQGQVAVRNVPMTGSISHVLVQLPHVFQRMEAQDLSSVIDARFHFFINKILPQYRDAVMSHTLIYV
PSYFDFVRLRNYFKKEELNFTHICEYTQKSGISRARHFFLQGEKQFLLLTERFHFYKRYTIKGIRNLIFY
ELPTYPHFYSEVCNMLRATSRGEEATWTCTVLYSKYDAQRLAAVVGVERAAQMLQSPKNVHLFVTGEK

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145415
ORF Size 2304 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145415.2, NP_663390.2
RefSeq Size 5548 bp
RefSeq ORF 2307 bp
Locus ID 215193
UniProt ID Q8BTT6
Cytogenetics 1 H6
Summary Regulates the p53 pathway to control the expansion growth of digestive organs.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145415" in other vectors (4)
SKU Description Size Price
MC221457 Diexf (untagged) - Mouse digestive organ expansion factor homolog (zebrafish) (Diexf), (10ug) 10 ug
$1,126.00
MR216881 Diexf (Myc-DDK-tagged) - Mouse digestive organ expansion factor homolog (zebrafish) (Diexf) 10 ug
$1,055.00
MR216881L3 Lenti ORF clone of Diexf (Myc-DDK-tagged) - Mouse digestive organ expansion factor homolog (zebrafish) (Diexf) 10 ug
$1,355.00
MR216881L4 Lenti ORF clone of Diexf (mGFP-tagged) - Mouse digestive organ expansion factor homolog (zebrafish) (Diexf) 10 ug
$1,355.00

file-alt Citations

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