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Pigw (NM_027388) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216866
Pigw (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis class W (Pigw) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $668.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Pigw or NM_027388"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pigw
Synonyms 2610044A17Rik; Gwt1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216866 representing NM_027388
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCAAAAGCAGCTGAAGGAAGCCTTTGTCAGGAACCTCAGTGGGACCAGTGTGCTGGAAGTGACCC
AGGGCCTGTGCTTTCCTGCCTTCTGTATCCTGTGTAGAGGCCTTTGGATCATCTTCTCACAGCACGTATG
CTCTTTCTCAAACACCTGGAGCACACGATTCCTCATGGACTTTGTTGTCTTGATAGTCCCCCTAGTGATC
ACTCTGACTGTATTGTCTTCCTTTATCCTCCTCGAGAATCTCACTGTGATTGTCTGGGGGGCATGGCTGT
TGTATCAGATATACCACAGGAGGACTTGCTACGCCAAAGTGCCTGTCCAGAAAGTCTTTGCAAACTTCTT
GAAAATCAGCCTAGAATCTGAGTACAATCCCGCAATCACCTGTTACCGGGTCATTAACAGCGTATTTACG
GCTATTGCCATTTTGGCCGTGGATTTCCCACTTTTCCCTAGAAGATTTGCCAAAACTGAACTTTATGGGA
CAGGAGCAATGGATTTTGGAGTAGGTGGCTTTATTTTTGGGGCGGCAATGGTTTGTCCAGAGGTTAGAAG
GAAATCTATAGAAGAGTCTAGATTTAATTATCTTAGAAAATCGCTGTATTCTGTCTGGCCATTGGTCTTC
TTAGGAATGGGACGGTTGGTCATTATAAAGTCCATAGGCTACCAGGAACATTCTACCGAGTACGGCATTC
ACTGGAACTTTTTCTTTACCATCATAGTTGTGCGATTAGTAACGTCACTGCTTTTGATTATTTTTCCTTT
AAATAAGTCGTGGATTGTGGCTGTCAGCATAACTGTGGTATACCAGCTAGCCCTGGATTATACTCCACTC
AAGAGGATACTTTTGTATGGCACTGATGGCAGTGGCACAAGGGTTGGCTTCTTAAATGCTAACCGAGAGG
GAATAATTTCCACTTTGGGGTATGTGACAATACACATGGCTGGTGTACAAACGGGATTGTATGTGCTTAA
GGGAAGAGCACAAGTCAGAGACTGGATAAAAGCTACTTGTTGGGTTTTCTCAGTAGCTGTTGGCTTCTTC
ATTTCTCTTCATATAGTGCAAGTAAACATAGAAGCAGTATCTCGAAGAATGGCCAATTTAGCCTTTTGTC
TGTGGGTAGTTGCTTCCAGTCTGATGCTTCTTAGTTGTCTGTTACTGAGTGGTATAATTTTGAGTTTTGC
CCAATTTCTAATTAAAGGGTCTCTAGTTCCATGTTCTTGGAAACTTATACAGTCTCCTACTACACATAAA
AACCATTCTGAATCTCTAATCTTAGAAGCTGAAAAAAACCAACCCAGTCTTTGTTTGATCACAGCTCTGA
ACAGAAACCAGCTGTTTTTTTTCTTACTGTCAAATATAACAACTGGTCTGATTAACCTGACGATGGATAC
ATTACACACTGGTGCCTTGTGGACCTTAGTTGTGCTCAGTATTTATATGTTTACCAACTGTTTAGTTATA
TATGTACTTGATTTACAAGGTAAAACCATAAAGTTTTGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216866 representing NM_027388
Red=Cloning site Green=Tags(s)

MSQKQLKEAFVRNLSGTSVLEVTQGLCFPAFCILCRGLWIIFSQHVCSFSNTWSTRFLMDFVVLIVPLVI
TLTVLSSFILLENLTVIVWGAWLLYQIYHRRTCYAKVPVQKVFANFLKISLESEYNPAITCYRVINSVFT
AIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFIFGAAMVCPEVRRKSIEESRFNYLRKSLYSVWPLVF
LGMGRLVIIKSIGYQEHSTEYGIHWNFFFTIIVVRLVTSLLLIIFPLNKSWIVAVSITVVYQLALDYTPL
KRILLYGTDGSGTRVGFLNANREGIISTLGYVTIHMAGVQTGLYVLKGRAQVRDWIKATCWVFSVAVGFF
ISLHIVQVNIEAVSRRMANLAFCLWVVASSLMLLSCLLLSGIILSFAQFLIKGSLVPCSWKLIQSPTTHK
NHSESLILEAEKNQPSLCLITALNRNQLFFFLLSNITTGLINLTMDTLHTGALWTLVVLSIYMFTNCLVI
YVLDLQGKTIKFW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027388
ORF Size 1509 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027388.2, NP_081664.2
RefSeq Size 2677 bp
RefSeq ORF 1512 bp
Locus ID 70325
UniProt ID Q8C398
Cytogenetics 11 C
Summary Required for the transport of GPI-anchored proteins to the plasma membrane. Probable acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor. Acetylation during GPI-anchor biosynthesis is not essential for the subsequent mannosylation and is usually removed soon after the attachment of GPIs to proteins.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_027388" in other vectors (4)
SKU Description Size Price
MC217010 Pigw (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class W (Pigw), transcript variant 1, (10ug) 10 ug
$516.00
MR216866 Pigw (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class W (Pigw), transcript variant 1 10 ug
$289.00 $468.00
MR216866L3 Lenti ORF clone of Pigw (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class W (Pigw), transcript variant 1 10 ug
$768.00
MR216866L4 Lenti ORF clone of Pigw (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class W (Pigw), transcript variant 1 10 ug
$768.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.