Cbr4 (NM_145595) Mouse Tagged ORF Clone

CAT#: MG216862

Cbr4 (tGFP-tagged) - Mouse carbonyl reductase 4 (Cbr4), (10ug)

Product Images

Circular map for MG216862

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cbr4
• Synonyms: A730083J17Rik; BC009118
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216862 representing NM_145595
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAAAGTCTGTGCGGTTTTTGGAGGCTCCCGGGGGATCGGCAGGGCGGTAGCCCAGCTAATGGCCC
  AGAAGGGTTACCGTCTGGCGATCGTCTCCAGAAACCTGGAAGTGGCCAAAGTCACCGCCGGCGAGCTCGG
  CGGAAACCACTTGGCATTTAGGTGTGATGTTGCCAAAGAACAAGATGTTCAGAGTACATTTCAAGAGATG
  GAGAAACATTTAGGTCCAGTAAATTTCTTGGTAAATGCAGCCGGTATCAACAGAGGCAGTCTTCTAGTAA
  GAACAAAGACTGAAGACATGATCTCTCAGCTGCACACTAACCTCCTGGGCTCCATGCTGACCTGTAAAGC
  TGCCATGAAGACAATGATTCAGCAGGGAGGGTCTATTGTTAATGTGGGAAGTATTATTGGTTTGAAAGGC
  AACGTTGGCCAGTCTGCATACAGTGCCACCAAAGGAGGACTCGTTGGGTTTTCACGCTCGCTTGCTAAAG
  AGGTTGCACGGAAGAAAATCAGAGTGAATGTGGTGGCACCAGGATTTATTCGCACGGATATGACAAGACA
  CTTGAAAGAAGAACACTTCAAGAAAAACATTCCTCTTGGGAGGTTTGGAGAAACTCTTGAGGTAGCACAT
  GCCGTTGTGTTTCTTTTAGAGTCACCATACATCACAGGCCATGTTCTTATCGTGGATGGAGGATTGCAGC
  TCACCGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216862 representing NM_145595
  Red=Cloning site Green=Tags(s)
  
  MDKVCAVFGGSRGIGRAVAQLMAQKGYRLAIVSRNLEVAKVTAGELGGNHLAFRCDVAKEQDVQSTFQEM
  EKHLGPVNFLVNAAGINRGSLLVRTKTEDMISQLHTNLLGSMLTCKAAMKTMIQQGGSIVNVGSIIGLKG
  NVGQSAYSATKGGLVGFSRSLAKEVARKKIRVNVVAPGFIRTDMTRHLKEEHFKKNIPLGRFGETLEVAH
  AVVFLLESPYITGHVLIVDGGLQLTV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145595
• ORF Size: 708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145595.1
• RefSeq Size: 1115 bp
• RefSeq ORF: 711 bp
• Locus ID: 234309
• UniProt ID: Q91VT4
• Cytogenetics: 8 B3.1
• Gene Summary: The heterotetramer with HSD17B8 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis. Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD. The homotetramer has NADPH-dependent quinone reductase activity. Both homotetramer and the heterotetramer have broad in vitro substrate specificity and can reduce 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones.[UniProtKB/Swiss-Prot Function]