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Rsg1 (NM_001081174) Mouse Tagged ORF Clone

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SKU
MG216859
Rsg1 (tGFP-tagged) - Mouse RIKEN cDNA 6330545A04 gene (6330545A04Rik), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Rsg1 or NM_001081174"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rsg1
Synonyms 6330545A04Rik; Gm723
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216859 representing NM_001081174
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGACCTCCCATGCACGGTTCGGTGATTGTCCCAGACTGGCATGAGACGGTCGAGGGTAAGGAGT
ACTTAGCCTGCATCCTGCGCAAGAATCGCCGGCGGGAGTTTGGGTTGCTTGAACGCCCGGTGCTGCCCCC
TTCCGTGGTCATTGATACCGCCAGCTATAAGATCTTTGTGTCCGGGAAGAGTGGTGTGGGCAAGACAGCA
CTGGTGGCAAAGCTGGCCGGCCTGGAAGTGCCTATAGTACACCATGAGACCACTGGCATTCAGACCACTG
TGGTGTTTTGGCCAGCCAAGCTGAAGGCCAGCGACTGTGTTGTCATGTTCCGTTTTGAATTCTGGGACTG
TGGGGAGTCTGCCCTCAAGAAGTTTGATCACATGCTGCCGGCTTGTAAAGAGAATGCAGATGCCTTCCTC
TTCCTCTTTTCTTTCACCGATCGTGCCTCCTTCGAAGACCTTCCTGGACAGTTGACTCGTGTAGCCGGTG
AAGCTCCTGGGCTCGTCAAGATAGTTATCGGGTCCAAGTTTGATCAGTACATGCACACAGATGTGCCAGC
GCGAGACCTCACAGCCTTCCGGCAGGCCTGGGAACTGCCCTTGTTCCGGGTGAAGAGTGTGCCAGGGCGG
CGGCTGGCAGACGGACGAACACTGGATGGCAGGGCTGGGCTAGCTGACACAGCCCATGTGCTCAATGGCC
TTGCAGAACAGTTGTGGCACCAGGACCAGGTGGCAGCCGGCCTGCTCCCCAGCTCCCCAGAGAGTGCTCC
CGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216859 representing NM_001081174
Red=Cloning site Green=Tags(s)

MARPPMHGSVIVPDWHETVEGKEYLACILRKNRRREFGLLERPVLPPSVVIDTASYKIFVSGKSGVGKTA
LVAKLAGLEVPIVHHETTGIQTTVVFWPAKLKASDCVVMFRFEFWDCGESALKKFDHMLPACKENADAFL
FLFSFTDRASFEDLPGQLTRVAGEAPGLVKIVIGSKFDQYMHTDVPARDLTAFRQAWELPLFRVKSVPGR
RLADGRTLDGRAGLADTAHVLNGLAEQLWHQDQVAAGLLPSSPESAPG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081174
ORF Size 774 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081174.2, NP_001074643.1
RefSeq Size 951 bp
RefSeq ORF 777 bp
Locus ID 76166
UniProt ID A2A825
Cytogenetics 4 D3
Summary Potential effector of the planar cell polarity signaling pathway. Plays a role in targeted membrane trafficking most probably at the level of vesicle fusion with membranes. Involved in cilium biogenesis by regulating the transport of cargo proteins to the basal body and to the apical tips of cilia. More generally involved in exocytosis in secretory cells (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081174" in other vectors (4)
SKU Description Size Price
MC211715 Rsg1 (untagged) - Mouse RIKEN cDNA 6330545A04 gene (6330545A04Rik), (10ug) 10 ug
$330.00
MR216859 Rsg1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 6330545A04 gene (6330545A04Rik) 10 ug
$289.00 $300.00
MR216859L3 Lenti ORF clone of Rsg1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 6330545A04 gene (6330545A04Rik) 10 ug
$600.00
MR216859L4 Lenti ORF clone of Rsg1 (mGFP-tagged) - Mouse RIKEN cDNA 6330545A04 gene (6330545A04Rik) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.