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Ogfod1 (NM_177767) Mouse Tagged ORF Clone

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SKU
MG216856
Ogfod1 (tGFP-tagged) - Mouse 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (Ogfod1) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $962.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ogfod1
Synonyms 4930415J21Rik; AA387199; AA939912; AW061076; mKIAA1612
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216856 representing NM_177767
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACGGGAAGCGGCCGGCGGATCCTGGCCCAGCGCGGCCAATGAAGAAGGGAAAGAAGCAGGTATCGG
CAGAGTTTTCGGACGCTGTCACTGAAGAAATCCTGAGGAAGCAGGTGGCTGAGGCCTGGAGCTGCCGGAC
ACCGTTCAGCCATGAAGCTATTGCCTTGGACATGGATCCTTTCCTTCACTGTGTGATCCCAAACTTCATC
CAAAGCCAAGACTTCTTGGAAGGACTTCACAAAGAGCTTTTGAGCCTGGACTTCCATGAAAAGTACAATG
ATTTATATAAGTTCCAACAGTCTGATGATTTGAAGAACCGAAAAGAGCCCCATATCTCAGCTTTAAGGAA
ACTTATGTTTGAAGATTTTCGGGCCTGGCTTTCTAAGGTTTCCGGCATTGACCTAGAACCAACCATTGAC
ATGTCGTGTGCGAAATACGAGTTCACTGATGCTCTGCTCTGCCATGATGATGAACTGGAGGGGCGCCGGA
TCGCTTTCATTCTGTACCTGGTTCCTTCTTGGGACAGGGACTTGGGGGGCACCCTGGACCTTTATGACAC
TGATGAACACTTGCAGCCAAAGCAGATTGTCAAGTCTCTTATCCCTTCCTGGAACAAACTGGTTTTCTTT
GAAGTGTCTCCAGTATCCTTTCACCAGGTGTCTGAAGTCCTGTCTGAAGAAACATCACGTTTGTCTATAA
GTGGCTGGTTTTATGGTCCTTCATTAACCAGGCCTCCTACCTACTTTGAGCCCCCAATCCCTCGAAACCC
TCACATCCCACAAGATCATGAAATTTTATATGAGTGGATCAACCCTGCTTACCTGGAAATGGATTATCAA
ATGCAAATTCAAGAAGAATTTGAAGAAAGGTCTGAAATTCTCCTGAAGGAGTTTCTTAAGCCTGAGAAAT
TTGCAGAGGTCTGTGAAGCCTTGGAGAAAGGAGATGTGGAATGGAAAAGCCATGGTCCCCCAAACAAAAG
GTTTTATGAGAAAGCAGAAGAAAATAACCTCCCTGACGTGCTGAAGGAATGCATGGGCCTGTTCCGCTCT
GAGGCGCTCTTCCTGCTGCTCTCCAACCTCACTGGCCTGAAGCTTCACTTCTTGGCCCCTTCAGAAGATG
ATGAGACTGAGGAGAAAGGAGAGGGGGAGACAGCCAGTGCTGCTGCTGGCACTGAAGAAGGGACTAGCCG
ACGCCCCTCGGGGCCAGAGAATAATCAGGTGGCCGCTGGCAGTCACAGTCAAGAGAACGGTGAACAGGCA
GACCCAGAAGCACAGGAAGAGGAAGCAAAGAAAGAATCGAGCGTTCCCATGTGCCAGGGGGAGCTGAGGC
GTTGGAAGACTGGCCATTACACATTAGTCCACGACAACACCAAGACTGAATTTGCCCTGGACCTGTTTCT
GTACTGTGGCTGTGAAGGCTGGGAGCCCGAATATGGCGGCTTTACCTCCTACATTGCTAAAGGTGAAGAT
GAAGAGCTGCTCATAGTGAATCCAGAAAACAACTCTTTGGCGTTGGTCTACAGAGACAGAGAAACGCTGA
GGTTTGTGAAACACATCAACCACCGCAGCCTAGAGCAGAGCAAAGCCTTCCCAAGCAGAAGCGGCTTCTG
GGACTTCGCATTCATCTACTATGAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216856 representing NM_177767
Red=Cloning site Green=Tags(s)

MNGKRPADPGPARPMKKGKKQVSAEFSDAVTEEILRKQVAEAWSCRTPFSHEAIALDMDPFLHCVIPNFI
QSQDFLEGLHKELLSLDFHEKYNDLYKFQQSDDLKNRKEPHISALRKLMFEDFRAWLSKVSGIDLEPTID
MSCAKYEFTDALLCHDDELEGRRIAFILYLVPSWDRDLGGTLDLYDTDEHLQPKQIVKSLIPSWNKLVFF
EVSPVSFHQVSEVLSEETSRLSISGWFYGPSLTRPPTYFEPPIPRNPHIPQDHEILYEWINPAYLEMDYQ
MQIQEEFEERSEILLKEFLKPEKFAEVCEALEKGDVEWKSHGPPNKRFYEKAEENNLPDVLKECMGLFRS
EALFLLLSNLTGLKLHFLAPSEDDETEEKGEGETASAAAGTEEGTSRRPSGPENNQVAAGSHSQENGEQA
DPEAQEEEAKKESSVPMCQGELRRWKTGHYTLVHDNTKTEFALDLFLYCGCEGWEPEYGGFTSYIAKGED
EELLIVNPENNSLALVYRDRETLRFVKHINHRSLEQSKAFPSRSGFWDFAFIYYE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177767
ORF Size 1635 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177767.4, NP_808435.3
RefSeq Size 5329 bp
RefSeq ORF 1638 bp
Locus ID 270086
UniProt ID Q3U0K8
Cytogenetics 8 C5
Summary Prolyl 3-hydroxylase that catalyzes 3-hydroxylation of 'Pro-62' of small ribosomal subunit uS12 (RPS23), thereby regulating protein translation termination efficiency. Involved in stress granule formation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_177767" in other vectors (4)
SKU Description Size Price
MC218900 Ogfod1 (untagged) - Mouse 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (Ogfod1), transcript variant 1, (10ug) 10 ug
$559.00
MR216856 Ogfod1 (Myc-DDK-tagged) - Mouse 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (Ogfod1), transcript variant 1 10 ug
$762.00
MR216856L3 Lenti ORF clone of Ogfod1 (Myc-DDK-tagged) - Mouse 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (Ogfod1), transcript variant 1 10 ug
$1,062.00
MR216856L4 Lenti ORF clone of Ogfod1 (mGFP-tagged) - Mouse 2-oxoglutarate and iron-dependent oxygenase domain containing 1 (Ogfod1), transcript variant 1 10 ug
$1,062.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.