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Epsti1 (NM_178825) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216826
Epsti1 (tGFP-tagged) - Mouse epithelial stromal interaction 1 (breast) (Epsti1) transcript variant b, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Epsti1 or NM_178825"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Epsti1
Synonyms 2310046K10Rik; 5033415K03Rik; BRESI1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216826 representing NM_178825
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACACCCGCAGTAAGGTGGTGGGCCCAGGCTTGGGCACTTCCTCCATCTCTCGGGACCACGCTGGAG
CTGGGCAGCGCAGGGAGCTGGGCTTGCAGCAAAACCGGAGACAGAGTTTGGAGGTGGCAGCTCCCGAGGG
CCCCAAGATGGAGAGGCAGGGGCATGCTGACCAGGGAAGCGCAGGCACATACACCCTGATAGCACCAAAC
GAGAGCAGAAGGCAGAAGATACAGAGAATTGCTGAGCAAGAACTGGCAGACCTGGAGAGATGGAAGCAAC
AGAACAGGGCAAAACCAGTCTACTTGGTGCCACAACGGCTGGGTGGAAGCCAGTCTGAGGCTGAAGTCAG
ACAGAAACAACAGCTGCAACAGATGCGTTCTAAATACCAGCAAAAGCTCAAGAGGGATGAATCTATTCGA
ATCAGGAAGGAAGCTGAGGAAGCCAAGTTCCAAAAAATGAAAGCAATCCAGAGGGAGAAGAGTAATAAAC
TAGAGGAGAAAAAGCAACTTCAAGAAGACATCAGAAGAGCCACATTTCGAGAGCATCATCAGTCCAAAAC
GGCTGAGCTCTTGAGCAGACTGGACACAGAGCGGAGAAACAGAAGTGCCTGTCTGATTGCTCCTCCTGCC
ACCCAGTCCTCGAGATGGGTGAGTGGAAACTCACGCAGGCTGCTGTCTGCCACCTTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216826 representing NM_178825
Red=Cloning site Green=Tags(s)

MYTRSKVVGPGLGTSSISRDHAGAGQRRELGLQQNRRQSLEVAAPEGPKMERQGHADQGSAGTYTLIAPN
ESRRQKIQRIAEQELADLERWKQQNRAKPVYLVPQRLGGSQSEAEVRQKQQLQQMRSKYQQKLKRDESIR
IRKEAEEAKFQKMKAIQREKSNKLEEKKQLQEDIRRATFREHHQSKTAELLSRLDTERRNRSACLIAPPA
TQSSRWVSGNSRRLLSATF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178825
ORF Size 687 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178825.4, NP_849147.1
RefSeq Size 1364 bp
RefSeq ORF 690 bp
Locus ID 108670
UniProt ID Q8VDI1
Cytogenetics 14 D3
Summary Plays a role in M1 macrophage polarization and is required for the proper regulation of gene expression during M1 versus M2 macrophage differentiation (PubMed:29217193). Might play a role in RELA/p65 and STAT1 phosphorylation and nuclear localization upon activation of macrophages (PubMed:29217193).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178825" in other vectors (3)
SKU Description Size Price
MR216826 Epsti1 (Myc-DDK-tagged) - Mouse epithelial stromal interaction 1 (breast) (Epsti1), transcript variant b 10 ug
$289.00 $300.00
MR216826L3 Lenti ORF clone of Epsti1 (Myc-DDK-tagged) - Mouse epithelial stromal interaction 1 (breast) (Epsti1), transcript variant b 10 ug
$600.00
MR216826L4 Lenti ORF clone of Epsti1 (mGFP-tagged) - Mouse epithelial stromal interaction 1 (breast) (Epsti1), transcript variant b 10 ug
$600.00

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