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Als2cl (NM_146228) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216819
Als2cl (tGFP-tagged) - Mouse ALS2 C-terminal like (Als2cl) transcript variant 3, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,072.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Als2cl
Synonyms 79H19D; 79H19E; BC022645; D930044G19Rik; mRn.49018
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216819 representing NM_146228
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTAGCTCTGAGGAGGCAGACCTGCTCCGGCTGGAGGAGGTCTTCTCAACGACTCTTGCTCGAACCA
TCAGTCTCATCCTGCAGCCTCTGCTCTTAGCTGACCCGGAGCCCTCAGATCCTTGTGGCAAAGAGTGCCT
TCGGCTTCTGCAGCAGCTGCATGAGAGTGCCCAGCGGTTGTGGTATGTGACAGAGCAAAGCCTGCTTTCG
CTGCGGCAGAGGCTATACCACCCACCTTCCAAGGGTCTGGAGGCCGTGCTGTTACTGAGCAATGCTGACC
ATGTCCTACAAGCCCACATGGAATACATCAAGTCCTACACTGATTGCGTGGTGGCGCAAGCCTTTCAGAA
GGTCTCAAAGAAGAGGAGTGAGTTCTGGAGGAGCCAGCGCAAGGCACTGAGGCAGCTACTGTCCTCTGGG
AGCTCTGAGGGCTCTGTGGGCACCACGATGTGCCAGGCCCTCCGCCAGCCACTCTCCCAGCACGTGCAGA
AATACTTGCTTCTCCTGCTGAGCCTCAGGGACACCCTTGATGAGAGCCACCCGGCCCAGGAGCTGGTGAT
GCATGCTATCACCCTGTTTGGAAACCTGCAGTCCTTCATGGGACAGGCCTTGGACCAAGCTGTGGCCACA
CAGGCCCTGTGGCACAGCCTCAGCAGCCGGCTGAGGGATGTGCTCTGCAGCCCTGCTCACCGACTCTTGC
AGGACAGCCAGGACATCCCCGTGGTGGTCACTCCACTGCGGGCTGAGCGCGTGCTGCTCTTTGATGACTC
GCTTGTCCTGTTGCAGGGCCACAATACCCACACGTTTGACCTGAAGTTGGTGTGGGTAAAACCCGGCCAA
GACAAGTGTGTGCTTCATATCCTCACTCCTGAAGAAGAGATCTCCTTCTGCACCAGAGACCCCCAGGGCC
AGGTTGTCTGGCAGTGGAAGGTGACCCAGGCTGTGTGCCAGGCCCTCTGTGGCAAGAAGGACTTCCCTGT
GCTGGGATCAGGCCGGGAGACGTCTGTGCCTCCTGAATGTCGCTGTGTGGCCTATACCTTCTGCCGAGAG
GGGCGGCTCTGCCAGGCTACCTATGATGGCGAGTGGTGTCGAGCTAAGCCCCATGGCAAGGGAACCCTGA
AGTGGCCAGATGGGCGGAACCACGTGGGGACTTTTTACCAGGGCTTGGAGCATGGCTTTGGCATCTGCCT
GGTGCCCCAGGCCTCTGAGGACAAGTTTGACTGTTACAAGTGCCACTGGCGGGAAGGCAGGATGTGTGAG
TACGGCATCTGTGAGTATGGCACTGACGAGGTGTACAAGGGCTATTTTCAGGCCGGTCTGCGGCACGGGT
TTGGCATCCTCGAGAGTGCCCCACAGGCTCCCCAGCCCTTCAGGTACACGGGCCACTGGGAGAGAGGCCA
GAGGAGTGGCTACGGCATCGAGGAGGACAGGGACAGGGGCGAGCGCTACATTGGCATGTGGCAGGCTGAC
CAGCGTCATGGCCCTGGGGTCGTGGTCACCCAGGCAGGCGTCTGCTATCAGGGCACATTCCAAGGGGACA
AGATGGCTGGCCCAGGCATCCTGCTCTGCGAGGACGACTCTCTGTACGAAGGTACTTTTACCAGGGACCT
GACACTCCTAGGGAAGGGCAAGGTCACCTTCCCCAATGGTTTTACCTTGGATGGTTCTTTCAGTAGTGGG
ACAGATAAAGGACTGTACACACAGGGAGTGCTGGACACGGCTGCCCTCCCACCTGACCCAAGCAGCACAC
GCAAGAGACAGCTGGGACTGGGCACCTTCCCTGTGGAGAGCCGCTGGCAGGGAGTCTACAGCCCCTTCCG
GGACTTCTTGCGTTTAGGCTGTCCTGGGGAACAGCAGGAAGCCCTCCTGGGCTTCCACACGCAGAGCTCC
AGGGAGCTACGCAAGTCCCAGGAGTGCCTGTGTTGTGAGAGGAGCCACCCCGAGGACTGTGTGGGCAGCA
TGGAAGATACCCTGAAGGAGCTGCTGCAGCACCGCAAGCCCAAGGCCCTGCAGCAGTACCTCAGGAAGGC
TCTGAGCAATTCCCGACACCCCCTGGGGCAGCTGCTCCGCACCCTGATGCTGACCTTCCAGGCCACATAT
TCAGGCATCGGGGCCAACAAACACCTGCAGGAGATGGCCCAGGAGGAGGTGAAGCAGCATGCCCGGGAGC
TTTGGGCTGCCTACAGGGGTCTACTGAAGGTTGCCTTGCAGCACCAGGGCCAGACCCTGGAGGAGGAGAA
CATGGAGACCAGGGACCTGCAGGTGCACGGGTTGCTCTTGCCCCTCATCCTGCCCAGCTTCTACTCAGAG
CTCTTCACACTGTACCTGCTTCTCCATGAGAGAGAGGATGGGCTGTACAGCCGGGGCATCACCAACCTCA
GCCTCTTCCCTGACACCAAGCTGCTGGAGTTCCTGGATGTGCAGAAGCATCTGTGGCCCCTCAAGGACCT
CAAGCTGACAAGCAATCAGAGATATTCTCTGGTCCGGGACAAGTGTTTCCTGACGGCCACTGAGTGTCTG
CAGAAGATCATCACCACAGTGCACCCTCGGGAGAAGCTGGAGGTACTTGAGAAGACCTATGGGGAAATTG
AGGCCACGGTGTCACGGGTGCTGGGCTGCAAATACAAACTGCCCATGGATGACCTATTGCCACTGCTTAT
CTATGTGGTGTCCCGAGCTCGAATTCAGCATCTGGGAGCTGAGATCCACCTGATCCGTGACATGATGGAT
CCCGTCCACACAGGTGGCCTCCATGACTTCCTGCTGACAGCTTTAGAGTCCTGTTATGAGCACATCCAGA
AGGAAGACATGAGGCCGCACCATCTGCCCGGGCACTGGGACGCTAGAGATCTCTGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216819 representing NM_146228
Red=Cloning site Green=Tags(s)

MSSSEEADLLRLEEVFSTTLARTISLILQPLLLADPEPSDPCGKECLRLLQQLHESAQRLWYVTEQSLLS
LRQRLYHPPSKGLEAVLLLSNADHVLQAHMEYIKSYTDCVVAQAFQKVSKKRSEFWRSQRKALRQLLSSG
SSEGSVGTTMCQALRQPLSQHVQKYLLLLLSLRDTLDESHPAQELVMHAITLFGNLQSFMGQALDQAVAT
QALWHSLSSRLRDVLCSPAHRLLQDSQDIPVVVTPLRAERVLLFDDSLVLLQGHNTHTFDLKLVWVKPGQ
DKCVLHILTPEEEISFCTRDPQGQVVWQWKVTQAVCQALCGKKDFPVLGSGRETSVPPECRCVAYTFCRE
GRLCQATYDGEWCRAKPHGKGTLKWPDGRNHVGTFYQGLEHGFGICLVPQASEDKFDCYKCHWREGRMCE
YGICEYGTDEVYKGYFQAGLRHGFGILESAPQAPQPFRYTGHWERGQRSGYGIEEDRDRGERYIGMWQAD
QRHGPGVVVTQAGVCYQGTFQGDKMAGPGILLCEDDSLYEGTFTRDLTLLGKGKVTFPNGFTLDGSFSSG
TDKGLYTQGVLDTAALPPDPSSTRKRQLGLGTFPVESRWQGVYSPFRDFLRLGCPGEQQEALLGFHTQSS
RELRKSQECLCCERSHPEDCVGSMEDTLKELLQHRKPKALQQYLRKALSNSRHPLGQLLRTLMLTFQATY
SGIGANKHLQEMAQEEVKQHARELWAAYRGLLKVALQHQGQTLEEENMETRDLQVHGLLLPLILPSFYSE
LFTLYLLLHEREDGLYSRGITNLSLFPDTKLLEFLDVQKHLWPLKDLKLTSNQRYSLVRDKCFLTATECL
QKIITTVHPREKLEVLEKTYGEIEATVSRVLGCKYKLPMDDLLPLLIYVVSRARIQHLGAEIHLIRDMMD
PVHTGGLHDFLLTALESCYEHIQKEDMRPHHLPGHWDARDLW

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146228
ORF Size 2856 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146228.3
RefSeq Size 5103 bp
RefSeq ORF 2859 bp
Locus ID 235633
UniProt ID Q60I26
Cytogenetics 9 60.79 cM
Summary Acts as a guanine nucleotide exchange factor (GEF) for Rab5 GTPase. Regulates the ALS2-mediated endosome dynamics (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_146228" in other vectors (4)
SKU Description Size Price
MC222813 Als2cl (untagged) - Mouse ALS2 C-terminal like (Als2cl), transcript variant 3, (10ug) 10 ug
$960.00
MR216819 Als2cl (Myc-DDK-tagged) - Mouse ALS2 C-terminal like (Als2cl), transcript variant 3 10 ug
$589.00 $872.00
MR216819L3 Lenti ORF clone of Als2cl (Myc-DDK-tagged) - Mouse ALS2 C-terminal like (Als2cl), transcript variant 3 10 ug
$1,172.00
MR216819L4 Lenti ORF clone of Als2cl (mGFP-tagged) - Mouse ALS2 C-terminal like (Als2cl), transcript variant 3 10 ug
$1,172.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.