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Ldb3 (NM_001039071) Mouse Tagged ORF Clone

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SKU
MG216809
Ldb3 (tGFP-tagged) - Mouse LIM domain binding 3 (Ldb3) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $877.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ldb3
Synonyms AW742271; PDLIM6; ZASP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216809 representing NM_001039071
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTACAGTGTGACTCTGACTGGGCCTGGGCCCTGGGGCTTCCGTCTGCAGGGAGGCAAGGACTTCA
ACATGCCCCTCACTATCTCCCGGATCACTCCAGGCAGCAAGGCAGCCCAGTCCCAGCTCAGCCAAGGAGA
CCTGGTGGTGGCCATTGATGGCGTCAACACAGACACCATGACCCACCTGGAGGCCCAGAACAAGATCAAG
TCGGCCAGCTACAACCTGAGCCTCACTCTACAGAAGTCCAAGCGGCCTATTCCCATCTCCACGACAGCGC
CTCCCATCCAGTCCCCGCTGCCAGTGATCCCCCACCAGAAGGACCCTGCTCTGGACACGAACGGCAGCCT
GGCGACTCCCAGCCCCAGCCCTGAGGCAAGGGCCAGCCCAGGCGCCCTGGAGTTCGGGGACACCTTTAGC
TCCTCCTTCTCCCAGACCTCTGTCTGCAGCCCACTCATGGAGGCCTCGGGGCCTGTCCTTCCTCTGGGTA
GCCCAGTGGCCAAGGCCAGCTCAGAGGGTGCACAGGGCTCAGTCAGCCCCAAAGTTCTGCCAGGCCCCAG
CCAACCGAGGCAATATAACAACCCCATTGGCCTGTACTCAGCAGAGACCCTGAGGGAGATGGCTCAGATG
TATCAGATGAGCCTCCGAGGGAAGGCCTCAGGTGCTGGACTCCTAGGAGGGAGCCTCCCTGTGAAAGACT
TGGCGGTGGACAGCGCCTCTCCTGTGTACCAGGCTGTGATCAAAACCCAAAGCAAGCCGGAAGATGAGGC
TGATGAGTGGGCCCGCCGGTCCTCCAACCTGCAGTCTCGCTCCTTCCGCATCCTGGCCCAGATGACCGGG
ACAGAATACATGCAAGACCCTGATGAAGAGGCTCTGCGAAGGTCAAGGCCACAGGCCTCTGCTTACAGTC
CTGCAGCCGCCGCTTCTCCAGCACCCTCTGCCCATACCAGCTACAGCGAGGGTCCAGCTGCCCCTGCACC
CAAGCCTCGGGTCGTAACCACTGCCAGCATCCGGCCTTCTGTCTACCAGCCAGTGCCTGCATCTTCCTAC
AGCCCATCTCCAGGAGCCAATTACAGTCCAACTCCCTATACCCCTTCGCCAGCTCCTGCTTATACCCCCT
CTCCTGCTCCCACCTATACCCCCTCACCTGCACCTACATACTCCCCTTCCCCTGCACCAGCTTATACCCC
CTCACCTGCCCCAAACTATACCCCTACACCATCAGCTGCCTACAGTGGGGGCCCTTCAGAATCTGCCAGC
CGTCCACCCTGGGTGACCGATGACAGCTTCTCTCAGAAGTTTGCTCCTGGAAAGAGCACTACCACTGTCA
GCAAGCAGACCCTGCCCCGGGGGGCTCCAGCCTACAACCCAACAGGTCCACAAGTGACACCTCTTGCCAG
GGGCACCTTCCAGAGAGCTGAGCGCTTCCCAGCTAGCAGCCGGACTCCCCTCTGTGGGCACTGTAACAAT
GTCATCAGGGGCCCTTTTCTGGTGGCCATGGGCCGCTCTTGGCACCCAGAAGAGTTCAATTGTGCCTACT
GCAAGACCTCCCTAGCAGATGTGTGCTTTGTGGAGGAGCAGAACAACGTTTACTGCGAGCGGTGTTACGA
GCAGTTCTTTGCCCCGATCTGTGCCAAATGTAACACCAAAATCATGGGGGAAGTGATGCACGCTCTGCGA
CAGACATGGCACACCACCTGCTTTGTCTGTGCAGCCTGCAAGAAGCCCTTTGGGAACAGCCTCTTCCACA
TGGAGGATGGAGAGCCATACTGTGAGAAAGACTACATCAACCTGTTCAGCACCAAGTGCCATGGATGCGA
TTTCCCTGTGGAAGCTGGTGACAAGTTTATCGAGGCCCTGGGGCACACCTGGCATGACACCTGCTTCATT
TGTGCGGTCTGCCACGTGAATCTGGAGGGTCAGCCATTCTACTCTAAGAAAGACAAACCACTGTGCAAGA
AGCACGCACATGCCATCAACGTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216809 representing NM_001039071
Red=Cloning site Green=Tags(s)

MSYSVTLTGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQSQLSQGDLVVAIDGVNTDTMTHLEAQNKIK
SASYNLSLTLQKSKRPIPISTTAPPIQSPLPVIPHQKDPALDTNGSLATPSPSPEARASPGALEFGDTFS
SSFSQTSVCSPLMEASGPVLPLGSPVAKASSEGAQGSVSPKVLPGPSQPRQYNNPIGLYSAETLREMAQM
YQMSLRGKASGAGLLGGSLPVKDLAVDSASPVYQAVIKTQSKPEDEADEWARRSSNLQSRSFRILAQMTG
TEYMQDPDEEALRRSRPQASAYSPAAAASPAPSAHTSYSEGPAAPAPKPRVVTTASIRPSVYQPVPASSY
SPSPGANYSPTPYTPSPAPAYTPSPAPTYTPSPAPTYSPSPAPAYTPSPAPNYTPTPSAAYSGGPSESAS
RPPWVTDDSFSQKFAPGKSTTTVSKQTLPRGAPAYNPTGPQVTPLARGTFQRAERFPASSRTPLCGHCNN
VIRGPFLVAMGRSWHPEEFNCAYCKTSLADVCFVEEQNNVYCERCYEQFFAPICAKCNTKIMGEVMHALR
QTWHTTCFVCAACKKPFGNSLFHMEDGEPYCEKDYINLFSTKCHGCDFPVEAGDKFIEALGHTWHDTCFI
CAVCHVNLEGQPFYSKKDKPLCKKHAHAINV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039071
ORF Size 1983 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039071.2, NP_001034160.1
RefSeq Size 4854 bp
RefSeq ORF 1986 bp
Locus ID 24131
UniProt ID Q9JKS4
Cytogenetics 14 B
Summary May function as an adapter in striated muscle to couple protein kinase C-mediated signaling via its LIM domains to the cytoskeleton.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001039071" in other vectors (4)
SKU Description Size Price
MC220170 Ldb3 (untagged) - Mouse LIM domain binding 3 (Ldb3), transcript variant 2, (10ug) 10 ug
$678.00
MR216809 Ldb3 (Myc-DDK-tagged) - Mouse LIM domain binding 3 (Ldb3), transcript variant 2 10 ug
$677.00
MR216809L3 Lenti ORF clone of Ldb3 (Myc-DDK-tagged) - Mouse LIM domain binding 3 (Ldb3), transcript variant 2 10 ug
$977.00
MR216809L4 Lenti ORF clone of Ldb3 (mGFP-tagged) - Mouse LIM domain binding 3 (Ldb3), transcript variant 2 10 ug
$977.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.