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Ap3m2 (NM_001122820) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216775
Ap3m2 (tGFP-tagged) - Mouse adaptor-related protein complex 3 mu 2 subunit (Ap3m2) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ap3m2
Synonyms 5830445E16Rik; AP-3B
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216775 representing NM_001122820
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTCACAGCCTTTTCTTGATTAACTCTTCCGGAGATATTTTCCTGGAGAAACACTGGAAAAGTGTGG
TCAGCCGCTCTGTGTGTGATTACTTCTTCGAGGCCCAAGAGAGAGCTACCGAGGCAGAAAATGTGCCTCC
GGTCATCCCCACCCCTCACCACTACCTCCTGAGTGTTTACCGGCACAAGATCTTCTTTGTGGCTGTAATC
CAGACAGAGGTCCCGCCTCTGTTTGTCATTGAATTCCTTCACCGAGTAGTGGATACATTTCAGGACTATT
TTGGGGTCTGTTCCGAGCCAGTGATCAAAGACAATGTGGTGGTGGTGTACGAGGTGTTGGAAGAGATGCT
GGACAACGGGTTCCCCTTGGCTACGGAGTCAAACATCCTCAAAGAACTGATAAAGCCTCCCACTATTCTC
CGGACAGTGGTCAACACCATCACAGGAAGCACCAATGTGGGGGACCAGCTTCCAACTGGGCAGCTGTCGG
TGGTGCCTTGGCGACGGACAGGGGTGAAATATACCAACAACGAGGCCTATTTCGATGTGGTGGAAGAGAT
AGACGCCATCATTGACAAGTCAGGTTCCACAGTCACCGCTGAGATCCAAGGGGTGATCGATGCCTGTGTT
AAGCTGACCGGGATGCCGGATCTCACTCTGTCCTTCATGAACCCCAGGTTGCTGGACGATGTTAGCTTCC
ACCCCTGTGTCCGTTTCAAGCGCTGGGAATCTGAGCGCATCCTCTCCTTCATCCCTCCTGACGGCAACTT
CCGCCTGCTCGCCTACCACGTCAGTGCACAGAACCTCGTTGCCATTCCAGTCTATGTCAAACATAGCATC
AGCTTCCGGGACAGCAGTTCACTTGGACGCTTTGAAATCACGGTGGGACCCAAGCAGACCATGGGGAAGA
CCATAGAGGGTGTGATTGTCACCAGCCAGATGCCCAAAGGAGTCCTGAATATGAGCCTCACTCCATCTCA
GGGGACACACACGTTTGACCCCGTGACAAAGATGCTGTCTTGGGATGTAGGAAAAATAAACCCACAGAAG
CTGCCAAGTTTGAAGGGGACGATGGGTCTCCAGGTCGGAGCTTCTAAACCAGATGAGAACCCTACGATTA
ACCTGCAGTTTAAGATCCAGCAGCTGGCCATTTCTGGACTCAAAGTGAACCGTCTGGATATGTACGGGGA
GAAGTACAAGCCCTTTAAAGGCATCAAATACATGACCAAAGCCGGCAAGTTCCAAGTCCGAACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216775 representing NM_001122820
Red=Cloning site Green=Tags(s)

MIHSLFLINSSGDIFLEKHWKSVVSRSVCDYFFEAQERATEAENVPPVIPTPHHYLLSVYRHKIFFVAVI
QTEVPPLFVIEFLHRVVDTFQDYFGVCSEPVIKDNVVVVYEVLEEMLDNGFPLATESNILKELIKPPTIL
RTVVNTITGSTNVGDQLPTGQLSVVPWRRTGVKYTNNEAYFDVVEEIDAIIDKSGSTVTAEIQGVIDACV
KLTGMPDLTLSFMNPRLLDDVSFHPCVRFKRWESERILSFIPPDGNFRLLAYHVSAQNLVAIPVYVKHSI
SFRDSSSLGRFEITVGPKQTMGKTIEGVIVTSQMPKGVLNMSLTPSQGTHTFDPVTKMLSWDVGKINPQK
LPSLKGTMGLQVGASKPDENPTINLQFKIQQLAISGLKVNRLDMYGEKYKPFKGIKYMTKAGKFQVRT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001122820
ORF Size 1254 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001122820.1, NP_001116292.1
RefSeq Size 3467 bp
RefSeq ORF 1257 bp
Locus ID 64933
UniProt ID Q8R2R9
Cytogenetics 8 A2
Summary Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Ap47 is a subunit of the plasma membrane adaptor (By similarity). In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001122820" in other vectors (3)
SKU Description Size Price
MR216775 Ap3m2 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant 1 10 ug
$289.00 $457.00
MR216775L3 Lenti ORF clone of Ap3m2 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant 1 10 ug
$757.00
MR216775L4 Lenti ORF clone of Ap3m2 (mGFP-tagged) - Mouse adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant 1 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.