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Akap4 (NM_009651) Mouse Tagged ORF Clone

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SKU
MG216759
Akap4 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 4 (Akap4) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $977.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Akap4 or NM_009651"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Akap4
Synonyms AKAP-4; AKAP82; Fsc1; mAKAP82; p82; PRKA4
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216759 representing NM_009651
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTGCCTACTGTGGTACTACAACGATGTCTGACGATATTGACTGGTTACACAGCCGCAGAGGCGTGT
GCAAGGTAGATCTTTACAGTCCAAAAGGACAACAAGATCAGGACCGAAAAGTGATATGCTTTGTGGATGT
GTCTACCTTGAATGTGGAAGATAAAGATTCCAAGGGTGCTGCTGGTTCCAGGTCAGAAGGCGAGTTAAAT
CTGGAGACTCTGGAAGAAAAAGAGATTATCGTAATCAAGGACACTGAAAAACAAGACCAGTCTAAGACGG
AGGGATCTGTGTGCCTTTTCAAACAAGCTCCCTCTGATCCTATAAGTGTCCTCAACTGGCTCCTCAATGA
TCTCCAGAAGTATGCCTTGGGTTTCCAACATGCATTAAGCCCCTCAGCCTCTAGCTGTAAACATAAAGTG
GGAGACCTAGAGGGTGACTATAGCAAAATACCATCTGAGAACTGCTACAGTGTGTACGCTGATCAAGTAA
ACTTTGATTATTTGAACAAAGGACCTCAAAACCTTCGCCTAGAAATGGCGGCATCCAAAAACACCAACAA
TAACCAAAGTCCCTCAAATCCTGCAACCAAATCTCCTAGCAATCAGAGGTCAGTTGCCACTCCTGAGGGA
GAATGTTCTATGGACGACCTTTCCTTCTATGTCAACCGATTGTCATCTCTGGTAATCCAAATGGCCCGTA
AGGAAATCAAGGACAAATTGGAAGGTGGAAGCAAATGTCTCCATCATTCCATGTATACATCAGGAGATAA
AGGGAAAACCAGCCCCCGGAGTGCTGTCAGCAAAATTGCTTCGGAGATGGCCCATGAAGCTGTTGAACTG
ACCTCATCAGAAATGCGTGGCAATGGAGAGGATTGCAGGGATGGCCGGAAAACCTTTTTGTATAGTGAAA
TGTGTAACAAGAACAAGTGTGGCGAAAAGCAGCAGATGTGCCCAAAAGACAGCAAAGAATTTGCGGATTC
CATCAGCAAGGGGCTTATGGTTTATGCAAATCAAGTAGCATCTGACATGATGGTCTCTGTTATGAAAACT
TTGAAAGTACACAGCTGTGGGAAGCCAATTCCGGCTTGTGTGGTCCTGAAGAGGGTACTATTGAAGCACA
CCAAGGAAATTGTATCTGATCTGATTGATTCATGTATGAAAAACTTGCATAACATAACAGGAGTCCTGAT
GACAGACTCAGACTTCGTTTCTGCTGTCAAGAGGAATCTTTTCAATCATGGTAAACAAAATGCAGCGGAC
ATCATGGAGGCCATGCTAAAGCGTCTGGTCAGTGCTCTTCTTGGTGAGAAGAAGGAGACTAAGTCTCAAA
GTCTGGCCTATGCAACCTTAAAAGCTGGAACTAATGATCCGAAATGCAAGAATCAGAGCCTTGAGTTCTC
AGCTATGAAAGCTGAAATGAAAGGAAAAGATAAATGCAAATCCAAAGCAGATCCATGCTGCAAATCGTTG
ACAAGTGCTGAGAGAGTCAGCGAGCATATCCTCAAAGAGAGCCTTACTATGTGGAACAACCAGAAGCAAG
GAAACCAAGGCAAAGTGACTAACAAAGTTTGCTGCACCAGTAAAGATGAGAAGAGAGAAAAGATCAGTCC
TTCCACAGATTCACTGGCCAAGGATCTAATTGTCTCTGCCCTTATGCTCATTCAATACCATCTGACCCAA
CAAGCCAAGGGCAAAGATCCATGTGAAGAGGAGTGCCCTGGTTCCTCCATGGGGTATATGTCCCAAAGTG
CACAATACGAAAAATGTGGAGGTGGCCAAAGTTCTAAATCACTTTCAATGAAGCATTTTGAAACTCGTGG
AGCTCCTGGACCATCTACATGTATGAAGGAAAATCAACTGGAGTCCCAGAAGATGGATATGTCAAACATG
GTTCTGTCCCTGATTCAGAAACTCCTGAGTGAGAGCCCTTTCAGTTGCGATGAACTAACTGAAAGTGACA
ATAAGCGTTGTTGTGATCCTAGATCAAGCAAAGCAGCTCCCATGGCCAAGAGACCTGAAGAGCAATGCCA
AGACAATGCAGAACTAGACTTCATCAGTGGGATGAAGCAAATGAACCGCCAGTTTATAGATCAGCTGGTA
GAATCCGTGATGAAACTCTGCCTGATCATGGCTAAGTACAGCAACAATGGAGCAGCCCTGGCTGAGCTGG
AAGAACAAGCAGCCCTGGTAGGCAGTGGCTCCAGATGTGGCCGTGATGCTATGATGTCACAAAATTATTC
TGAAACTCCTGGCCCTGAAGTTATTGTCAACAATCAGTGCTCTACAACTAACTTGCAGAAGCAGCTGCAG
GCTGTCCTGCAATGGATTGCAGCCTCTCAATTCAATGTGCCCATGCTCTACTTCATGGGAGACGATGATG
GACAACTAGAGAAGCTCCCTGAAGTTTCAGCTAAGGCTGCAGAGAAGGGGTACAGTGTAGGAGATCTTCT
TCAGGAGGTCATGAAGTTTGCCAAAGAACGACAACTGGATGAAGCCGTGGGCAACATGGCTAGAAAGCAG
CTGCTAGACTGGCTTCTCGCTAACCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216759 representing NM_009651
Red=Cloning site Green=Tags(s)

MIAYCGTTTMSDDIDWLHSRRGVCKVDLYSPKGQQDQDRKVICFVDVSTLNVEDKDSKGAAGSRSEGELN
LETLEEKEIIVIKDTEKQDQSKTEGSVCLFKQAPSDPISVLNWLLNDLQKYALGFQHALSPSASSCKHKV
GDLEGDYSKIPSENCYSVYADQVNFDYLNKGPQNLRLEMAASKNTNNNQSPSNPATKSPSNQRSVATPEG
ECSMDDLSFYVNRLSSLVIQMARKEIKDKLEGGSKCLHHSMYTSGDKGKTSPRSAVSKIASEMAHEAVEL
TSSEMRGNGEDCRDGRKTFLYSEMCNKNKCGEKQQMCPKDSKEFADSISKGLMVYANQVASDMMVSVMKT
LKVHSCGKPIPACVVLKRVLLKHTKEIVSDLIDSCMKNLHNITGVLMTDSDFVSAVKRNLFNHGKQNAAD
IMEAMLKRLVSALLGEKKETKSQSLAYATLKAGTNDPKCKNQSLEFSAMKAEMKGKDKCKSKADPCCKSL
TSAERVSEHILKESLTMWNNQKQGNQGKVTNKVCCTSKDEKREKISPSTDSLAKDLIVSALMLIQYHLTQ
QAKGKDPCEEECPGSSMGYMSQSAQYEKCGGGQSSKSLSMKHFETRGAPGPSTCMKENQLESQKMDMSNM
VLSLIQKLLSESPFSCDELTESDNKRCCDPRSSKAAPMAKRPEEQCQDNAELDFISGMKQMNRQFIDQLV
ESVMKLCLIMAKYSNNGAALAELEEQAALVGSGSRCGRDAMMSQNYSETPGPEVIVNNQCSTTNLQKQLQ
AVLQWIAASQFNVPMLYFMGDDDGQLEKLPEVSAKAAEKGYSVGDLLQEVMKFAKERQLDEAVGNMARKQ
LLDWLLANL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009651
ORF Size 2547 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009651.4, NP_033781.2
RefSeq Size 2851 bp
RefSeq ORF 2550 bp
Locus ID 11643
UniProt ID Q60662
Cytogenetics X 3.17 cM
Summary Major structural component of sperm fibrous sheath. Plays a role in sperm motility (PubMed:12167408).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009651" in other vectors (4)
SKU Description Size Price
MC222038 Akap4 (untagged) - Mouse A kinase (PRKA) anchor protein 4 (Akap4), transcript variant 1, (10ug) 10 ug
$856.00
MR216759 Akap4 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 4 (Akap4), transcript variant 1 10 ug
$589.00 $777.00
MR216759L3 Lenti ORF clone of Akap4 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 4 (Akap4), transcript variant 1 10 ug
$1,077.00
MR216759L4 Lenti ORF clone of Akap4 (mGFP-tagged) - Mouse A kinase (PRKA) anchor protein 4 (Akap4), transcript variant 1 10 ug
$1,077.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.