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Rufy4 (NM_001170641) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216756
Rufy4 (tGFP-tagged) - Mouse RUN and FYVE domain containing 4 (Rufy4) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $725.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rufy4
Synonyms F930048N03Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216756 representing NM_001170641
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAACAACGGGACCATCCTCAAGATCAGCAGGAGCCTGAAAAATGCCGTCTCTGCCATCCTCCAGG
GATATGGGGACGGGCAGGAGCCAGTGACAGACACCAGCGCTGAGCTACACAGACTCTGTGGCTGCCTGGA
GCTGCTACTGCAGTTTGATCAGAAAGAGCAGAGGAGCTTCCTGGGGGCCCGGAAGGATTACTGGGACTTC
CTATTCACTGCCCTTCGACGGCACCGGGGATACACAGAGCAGATGAGCTTCATATGCTCACAGGACAAGC
TGAAGACCTCACTAGGAAAAGGCCGTGCCTTTATCCGACTCTGCCTTGCCCGAGGCCAGCTGGCTGAGTC
GATGCAGCTGTGCCTCCTGAACCCACAACTCACCAGGGAATGGTATGGCCCTCGGAGCCCTCTGTTGTGC
CCTGAGCTCCAGGAAGACATCTTGGACTCTCTCTATGCCCTCAATGGAGTTGCCTTCAACTTGGACCTGC
AGCGGCCAGACCTGGATGAAGCCTGGCCCATGTTCTCAGAGTCCCGCTGTTCCAGTCCCAGCCGGACCGG
GAAAAGAAGACCCGGGAAACCCAAAGGTTTTCCAGAGGAGGTGAGATGCTCCAGGGGAGAGCAGCTGCAG
GAACCAGACACTGGAGGAACCAGCTGTCTGCAGGATGCAACCAGAGAAGACCGCACACCTGACCTCTGTA
AACCCTTGCAGCCAAGCCATCTTCCCACCTTTCTGGAAGAGAAGAGGGAGGATTCCAGAAGCCTCAGTTG
TCCCCAGAGCACTTGGGAAACAGAAAGGGAAGGATTTCAGCTAGACCAGAAGGATGGAGGCCCAAAGCCC
AGGAAATTCCTGGAAAACTCAACAGCCAGCATCCAGCAACAGAGGAGCAGGGCTAAGGATGTTAAAATGC
AGCTGACAGGAAGGAAAGTTGAAGGAAAAGGAAGTCTGTCGGGGACAGAAGATCAGAGAACAACAGAAGG
GATCCAGAAAAGGGCAGCAGATTGGGACCTTGGCCAGGGGCTGATGGCCCCCGGTCTTCAGGGAAGAGAA
GATGCAGAGTTAGGATACAGGTGTGAGTGGAATCAGCCTGATGTTCTCAGGCAATCCTGGGTCCTGGGAA
CAAAGAAAAGTTCTCCCACAGAGAAGCCACAAGAGTGGACAGGAGTGACCAGTGGAACCATGCAGGAGGA
TGGGTCAGAAGTGCCCTTGCAGCAGGAGGTGATCAAGGACCCTGGGTATGGGCTCCAGCTGGCAAAGGAA
CAGGCCCAGTGCCAGGAGCAGCTGCGGGCACAGGAAGCAGAACTTCAGGCGCTCCAGGAGCAGCTCAGCA
GGTGTCAAAAAGAAAGAGCCCTGTTGCAAGTGAAGCTGGAGCAGAAGCAGCAGGAGGCTGAGAGGAGGGA
CGCCATGTATCAGACAGAGCTTGAAGGGCAGAGGGACCTGGTCCAGGCCATGAAAAGGAGGGTGTTGGAA
CTGATCCATGAGAAGGACCTTCAGTGGCAGAGACTCCAGCAGCTGTCTACTGTGGCCCCTGGACACTGCA
TAGGCTGCAACAAGGTCTTCAGGCGTCTGTCTCGGCGGTATCCTTGCAGGCTCTGTGGAGGCCTGGTTTG
CCACGCCTGCTCTGTAGACTACAAGAAGAGAGAGCGTTGCTGCCCAACCTGTGCCCAGCAGGAAGAAATC
CAGGACACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216756 representing NM_001170641
Red=Cloning site Green=Tags(s)

MANNGTILKISRSLKNAVSAILQGYGDGQEPVTDTSAELHRLCGCLELLLQFDQKEQRSFLGARKDYWDF
LFTALRRHRGYTEQMSFICSQDKLKTSLGKGRAFIRLCLARGQLAESMQLCLLNPQLTREWYGPRSPLLC
PELQEDILDSLYALNGVAFNLDLQRPDLDEAWPMFSESRCSSPSRTGKRRPGKPKGFPEEVRCSRGEQLQ
EPDTGGTSCLQDATREDRTPDLCKPLQPSHLPTFLEEKREDSRSLSCPQSTWETEREGFQLDQKDGGPKP
RKFLENSTASIQQQRSRAKDVKMQLTGRKVEGKGSLSGTEDQRTTEGIQKRAADWDLGQGLMAPGLQGRE
DAELGYRCEWNQPDVLRQSWVLGTKKSSPTEKPQEWTGVTSGTMQEDGSEVPLQQEVIKDPGYGLQLAKE
QAQCQEQLRAQEAELQALQEQLSRCQKERALLQVKLEQKQQEAERRDAMYQTELEGQRDLVQAMKRRVLE
LIHEKDLQWQRLQQLSTVAPGHCIGCNKVFRRLSRRYPCRLCGGLVCHACSVDYKKRERCCPTCAQQEEI
QDT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001170641
ORF Size 1689 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001170641.1, NP_001164112.1
RefSeq Size 2336 bp
RefSeq ORF 1692 bp
Locus ID 435626
UniProt ID Q3TYX8
Cytogenetics 1 C3
Summary Positively regulates macroautophagy in primary dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001170641" in other vectors (4)
SKU Description Size Price
MC219141 Rufy4 (untagged) - Mouse RUN and FYVE domain containing 4 (Rufy4), transcript variant 1, (10ug) 10 ug
$577.00
MR216756 Rufy4 (Myc-DDK-tagged) - Mouse RUN and FYVE domain containing 4 (Rufy4), transcript variant 1 10 ug
$289.00 $525.00
MR216756L3 Lenti ORF clone of Rufy4 (Myc-DDK-tagged) - Mouse RUN and FYVE domain containing 4 (Rufy4), transcript variant 1 10 ug
$825.00
MR216756L4 Lenti ORF clone of Rufy4 (mGFP-tagged) - Mouse RUN and FYVE domain containing 4 (Rufy4), transcript variant 1 10 ug
$825.00

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