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Tm7sf2 (NM_028454) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216738
Tm7sf2 (tGFP-tagged) - Mouse transmembrane 7 superfamily member 2 (Tm7sf2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tm7sf2
Synonyms 3110041O18Rik; ANG1; C14SR; Dhcr14
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216738 representing NM_028454
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTTCTCGTGAGGCCTCCCAGGCCCCACTGGAATTCGGGGGGCCGTTGGGCGTCGCGGCTTTACTGA
TCCTGCTGCCTGCCACCATGTTCCACCTGCTCCTGGCGGCTCGCTCGGGCCCGGCGCGCCTCCTGGCCCT
ACCGGCCTATCTGCCTGGGCTGGAGGAGCTGTGGAGCCCATGGGCTCTGCTGCTATTGTTCATCTGGCTC
GGCCTGCAGGTGGCGCTCTATTTGCTGCCTGCACGCAAGGTGGCCGAAGGCCTGGAACTGAAGGACAAGA
GTCGCCTGCGCTACCCTATTAATGGCTTCCAGGCTCTGGTGCTAACAGCCCTGTTGATGGGGCTGGGGGT
GTCAGTGGGGCTGCCCCTGGGGGCACTCCCTGGAATGCTCCTGCCCTTGGCCTTTGCGACCACTCTCACC
AGCTTTATCTTCAGCCTCCTCCTCTATGCGAAGGCTTTGGTAGCTCCTGCCTCAGCCCTGGCTCCTGGGG
GAAACTCAGGAAATTCCATGTATGACTTCTTTCTTGGACGGGAGCTGAACCCTCGCCTCGGTTCCTTTGA
CTTCAAATATTTCTGTGAGCTGAGACCTGGCCTCATTGGCTGGGTTTTCATTAACCTGGCCCTGCTGATG
CAGGAGGCAGAGCTTCGGGGGAGTCCTTCCCTGGCTATGTGGCTGGTCAATGGCTTCCAGTTGCTGTATG
TGGGTGATGCCCTCTGGTATGAGGAGTCTGTCCTCACCACCATGGACATAATACATGATGGTTTTGGCTT
CATGCTGGTCTTTGGAGATCTAGCTTGGGTACCATTCACCTACAGCCTGCAGGCCCAGTTCCTGTTGTAC
CATCCACAGCCTCTGGGGTTGCCCATGGCCTTGCTCATCTGCCTCCTTAAGGTTATTGGTTACTACATCT
TCCGAGGGGCCAACTCCCAGAAAAACACATTCAGGAAGAATCCTTCTGACCCCAGCGTGGCTGGTCTTGA
GACCATCCCGACTGCCACGGGGAGGCAGCTGCTGGTGTCTGGGTGGTGGGGTATGGTTCGACACCCCAAC
TACCTGGGAGATCTCATCATGGCTCTGGCATGGTCTTTGCCCTGTGGGCTATCCCATCTGCTGCCCTACT
TCTACGTCCTCTACTTCACTGCACTGCTGGTGCACCGCGAGGCCAGAGATGAGCAGCAGTGCCTCCAAAA
GTATGGCCGTGCCTGGCAGGAGTACTGCAAGCGTGTGCCTTACCGAATCATACCCTATGTCTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216738 representing NM_028454
Red=Cloning site Green=Tags(s)

MTSREASQAPLEFGGPLGVAALLILLPATMFHLLLAARSGPARLLALPAYLPGLEELWSPWALLLLFIWL
GLQVALYLLPARKVAEGLELKDKSRLRYPINGFQALVLTALLMGLGVSVGLPLGALPGMLLPLAFATTLT
SFIFSLLLYAKALVAPASALAPGGNSGNSMYDFFLGRELNPRLGSFDFKYFCELRPGLIGWVFINLALLM
QEAELRGSPSLAMWLVNGFQLLYVGDALWYEESVLTTMDIIHDGFGFMLVFGDLAWVPFTYSLQAQFLLY
HPQPLGLPMALLICLLKVIGYYIFRGANSQKNTFRKNPSDPSVAGLETIPTATGRQLLVSGWWGMVRHPN
YLGDLIMALAWSLPCGLSHLLPYFYVLYFTALLVHREARDEQQCLQKYGRAWQEYCKRVPYRIIPYVY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028454
ORF Size 1254 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028454.2, NP_082730.2
RefSeq Size 1496 bp
RefSeq ORF 1257 bp
Locus ID 73166
UniProt ID Q71KT5
Cytogenetics 19 A
Summary Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028454" in other vectors (4)
SKU Description Size Price
MC211388 Tm7sf2 (untagged) - Mouse transmembrane 7 superfamily member 2 (Tm7sf2), (10ug) 10 ug
$503.00
MR216738 Tm7sf2 (Myc-DDK-tagged) - Mouse transmembrane 7 superfamily member 2 (Tm7sf2) 10 ug
$289.00 $457.00
MR216738L3 Lenti ORF clone of Tm7sf2 (Myc-DDK-tagged) - Mouse transmembrane 7 superfamily member 2 (Tm7sf2) 10 ug
$757.00
MR216738L4 Lenti ORF clone of Tm7sf2 (mGFP-tagged) - Mouse transmembrane 7 superfamily member 2 (Tm7sf2) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.