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Gpx5 (NM_010343) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216722
Gpx5 (tGFP-tagged) - Mouse glutathione peroxidase 5 (Gpx5), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpx5
Synonyms Arep; AV379049
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216722 representing NM_010343
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTACAGAGTTAAGAGTCTTCTATCTTGTTCCACTTCTTCTAGCCAGCTATGTGCAGACAACCCCCA
GGCCGGAAAAGATGAAGATGGACTGCTACAAAGACGTGAAAGGCACCATCTACGACTATGAGGCTCTGTC
TCTTAATGGGAAGGAACACATTCCATTCAAGCAGTATGCAGGAAAGCACGTCCTCTTTGTCAATGTGGCT
ACCTATTGCGGTCTGACAATCCAGTACCCTGAGCTGAATGCACTCCAGGAGGATCTGAAGCCATTTGGCT
TGGTTATATTGGGCTTTCCCTGCAACCAATTTGGAAAGCAAGAACCAGGAGACAATTTAGAGATTCTTCC
TGGGCTCAAGTATGTTCGTCCAGGAAAAGGGTTTTTACCTAACTTCCAGCTTTTTGCAAAAGGGGATGTA
AATGGTGAAAACGAGCAGAAAATCTTCACCTTCTTGAAGCGTTCTTGTCCTCACCCCTCAGAGACTGTGG
TCATGAGCAAACATACCTTCTGGGAGCCAATAAAAGTCCATGACATCCGCTGGAACTTTGAGAAGTTCCT
GGTGGGACCCGATGGCGTCCCTGTCATGCGCTGGTTCCACCAGGCTCCTGTCAGCACTGTCAAGTCTGAC
ATCATGGCGTACCTGAGCCATTTCAAAACCATA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216722 representing NM_010343
Red=Cloning site Green=Tags(s)

MVTELRVFYLVPLLLASYVQTTPRPEKMKMDCYKDVKGTIYDYEALSLNGKEHIPFKQYAGKHVLFVNVA
TYCGLTIQYPELNALQEDLKPFGLVILGFPCNQFGKQEPGDNLEILPGLKYVRPGKGFLPNFQLFAKGDV
NGENEQKIFTFLKRSCPHPSETVVMSKHTFWEPIKVHDIRWNFEKFLVGPDGVPVMRWFHQAPVSTVKSD
IMAYLSHFKTI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010343
ORF Size 663 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010343.2, NP_034473.2
RefSeq Size 1629 bp
RefSeq ORF 666 bp
Locus ID 14780
UniProt ID P21765
Cytogenetics 13 7.73 cM
Summary Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010343" in other vectors (4)
SKU Description Size Price
MC208596 Gpx5 (untagged) - Mouse glutathione peroxidase 5 (Gpx5), (10ug) 10 ug
$330.00
MR216722 Gpx5 (Myc-DDK-tagged) - Mouse glutathione peroxidase 5 (Gpx5) 10 ug
$289.00 $300.00
MR216722L3 Lenti ORF clone of Gpx5 (Myc-DDK-tagged) - Mouse glutathione peroxidase 5 (Gpx5) 10 ug
$600.00
MR216722L4 Lenti ORF clone of Gpx5 (mGFP-tagged) - Mouse glutathione peroxidase 5 (Gpx5) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.