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Spats1 (NM_027649) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216721
Spats1 (tGFP-tagged) - Mouse spermatogenesis associated serine-rich 1 (Spats1), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Spats1 or NM_027649"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spats1
Synonyms 1700011H05Rik; 4933400B06Rik; Daip2; Ddip; Srsp1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216721 representing NM_027649
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCTTCCAAGGATACCCAGCACGGTGATGCTCTAGAATCTAAAAGTTGCTTGGCTAACAGGACGT
CCTCTCGTCAGAACAAAAGGACCAGCCTCTCGTCTTCTGACGGAACAGGTCCGAGAGTCACTGAGTCCCT
AGGCCTTCCCAGGGTGTTGACTCCCTCTGACACGGCTGCGGAGCTGGGTCAGAAGACCTCCTCCTCTTCC
TCTTCCTCCTCTTCCTCTGCTCAGTCTAATCGTTCCTCAAAAGTTTCGCTGCCTGAAATCCCAAAGGAAA
AATATCCTGAGGAATTCAGCCTGCTCAATTCACAGACAGAAGATGGGCAGCGTCCTGAGTGGACATTTTA
TCCAAGGTTCAGCAGCAACATCCACACCTACCACATTGGAAAGCAGTGCTTCTTCAATGGGGTCTTCCGG
GGCAACAGGAGGTCTGTGGCAGAGAGGACAGTGGACAACAGCCTCGGGAAGAAGAAATACGATATTGATC
CCAGAAATGGAATCCCCAAATTGACACCAGGGGATAATCCGTACATGTTCCCAGAACAGAGTAAAGAGTT
CTTCAAAGCGGGAGCAACTTTGCCTCCGGTGAACTTCTCACTGGGACCTTACGAGAAAAAATTCGACACA
TTTATCCCGCTTGAGCCACTTCCAAAAATTCCCAATCTGCCTTTCTGGGAGAAGGAAAAAGCCAACAATT
TGAAGAACGAGATAAAAGAAGTCGAGGAGCTTGACAACTGGCAGGTGCCGATGCCCTTCCTACATGGTTT
CTTCTCCACTGGTGCTTCCAACTTTTCAAGACAACAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216721 representing NM_027649
Red=Cloning site Green=Tags(s)

MESSKDTQHGDALESKSCLANRTSSRQNKRTSLSSSDGTGPRVTESLGLPRVLTPSDTAAELGQKTSSSS
SSSSSSAQSNRSSKVSLPEIPKEKYPEEFSLLNSQTEDGQRPEWTFYPRFSSNIHTYHIGKQCFFNGVFR
GNRRSVAERTVDNSLGKKKYDIDPRNGIPKLTPGDNPYMFPEQSKEFFKAGATLPPVNFSLGPYEKKFDT
FIPLEPLPKIPNLPFWEKEKANNLKNEIKEVEELDNWQVPMPFLHGFFSTGASNFSRQQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027649
ORF Size 807 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027649.3, NP_081925.2
RefSeq Size 1160 bp
RefSeq ORF 810 bp
Locus ID 71020
UniProt ID A2RRY8
Cytogenetics 17 B3
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Documents
Resources
"NM_027649" in other vectors (4)
SKU Description Size Price
MC211125 Spats1 (untagged) - Mouse spermatogenesis associated, serine-rich 1 (Spats1), (10ug) 10 ug
$330.00
MR216721 Spats1 (Myc-DDK-tagged) - Mouse spermatogenesis associated, serine-rich 1 (Spats1) 10 ug
$289.00 $300.00
MR216721L3 Lenti ORF clone of Spats1 (Myc-DDK-tagged) - Mouse spermatogenesis associated, serine-rich 1 (Spats1) 10 ug
$600.00
MR216721L4 Lenti ORF clone of Spats1 (mGFP-tagged) - Mouse spermatogenesis associated, serine-rich 1 (Spats1) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.